Immunohistochemistry Antibodies | St John's Laboratory

Formalin-fixed, paraffin-embedded human liposarcoma stained with anti-CDK4 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear/cytoplasmic staining of tumor cells.

Anti-CDK4 antibody [ZR394] (STJ180606)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £566.50

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Western blot analysis of HepG2 cells using Cdk5 Polyclonal Antibody diluted at 1：500

Anti-CDK5 antibody (1-50 aa) (STJ92200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot of rat striatal lysate showing specific immunolabeling of the ~28 kDa Cdk5 protein.

Anti-CDK5 antibody [1H3] (STJA0003592)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£454.50

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Western blot analysis of1) Hela Cell, 2) Jurkat Cell, 3) Mouse Brain Tissue, 4) Rat Brain Tissue lysate using mouse monoclonal antibody diluted at 1:2,000.

Anti-CDK5 antibody [3D1] (STJ196638)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-CDK5 antibody [PT1229R] (STJA0040176)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK5 antibody [SRM14897] (STJ11109627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of Hela cell. 1, p35 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK5R1 antibody (11-60 aa) (STJ94875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using p39 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDK5R2 antibody (81-130 aa) (STJ94880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded mouse kidney using CDK5RAP1 Rabbit polyclonal antibody (STJ118289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK5RAP1 antibody (1-350) (STJ118289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CDK5RAP1 antibody (251-300 aa) (STJ92201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using CDK5RAP2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CDK5RAP2 antibody (251-300 aa) (STJ92202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using HSF-27 Polyclonal Antibody diluted at 1：2000

Anti-CDK5RAP3 antibody (341-390 aa) (STJ93603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g CDK6 antibody (STJ11103846). Western blot was performed from the immunoprecipitate using CDK6 antibody (STJ11103846) at a dilution of 1:1000.

Anti-CDK6 antibody (226-326) (STJ11103846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-CDK6 antibody (280-325 aa) (STJ98721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-CDK6 antibody [PT0069R] (STJA0039694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Western blot analysis of various cells using Cdk7 Polyclonal Antibody

Anti-CDK7 antibody (291-340 aa) (STJ92203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Cdk8 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDK8 antibody (1-50 aa) (STJ92205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded rat liver using CDK9 Rabbit polyclonal antibody (STJ114907) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK9 antibody (153-372) (STJ114907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g CDK9 rabbit monoclonal antibody (STJ11101280) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of CDK9 in representative gene loci (RAC1) , as shown in figure.

Anti-CDK9 antibody (273-372) [S0MR] (STJ11101280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using Cdk9 monoclonal antibody against HEK293 (1) and CDK9-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CDK9 antibody [1B5A7] (STJ97949)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-Cdk9 antibody [PT1054R] (STJA0039967)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using KKIALRE Polyclonal Antibody diluted at 1：500

Anti-CDKL1 antibody (281-330 aa) (STJ93846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat and 293 cells, using CDKL2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CDKL2 antibody (211-260 aa) (STJ93847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of CDKL3 in paraffin-embedded Human colon carcinoma using CDKL3 Rabbit polyclonal antibody (STJ119103) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDKL3 antibody (1-120) (STJ119103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using CDKL3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CDKL3 antibody (291-340) (STJ94499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using CdkL4 Polyclonal Antibody diluted at 1：500

Anti-CDKL4 antibody (266-315 aa) (STJ92207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Whole cell lysates of A549 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21 (PTR2559) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 21kDaObserved band size: 21kDa

Anti-CDKN1A antibody (1-100 aa) (STJ196154)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of CDKN1A/p21CIP1 in paraffin-embedded human breast cancer using CDKN1A/p21CIP1 Rabbit polyclonal antibody (STJ111165) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDKN1A antibody (1-164) (STJ111165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of MCF7, using CDKN1A/p21CIP1 antibody (STJ23069) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDKN1A antibody (1-164) (STJ23069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CDKN1A antibody (1-164) [S1MR] (STJ11101631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, p21 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDKN1A antibody (111-160 aa) (STJ94862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human

£319.50

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Anti-CDKN1A/p21CIP1 antibody [SRM14465] (STJ11109195)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (STJ119057) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-CDKN1B antibody (1-198) (STJ119057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 antibody (STJ23072) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDKN1B antibody (1-198) (STJ23072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p27Kip1 (ABT-p27) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 22kDaObserved band size: 24kDa

Anti-CDKN1B antibody (100-198 aa) [ABT-p27] (STJ196998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human

£0.00

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, p27 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CDKN1B antibody (149-198 aa) (STJ94866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Cow

£49.00 - £261.00

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Human B-cell lymphoma stained with anti-p27 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Anti-CDKN1B antibody [SX53G8] (STJ180205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £492.50

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Anti-CDKN1B/p27KIP1 antibody [SRM13057] (STJ11107787)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Human placenta tissue was stained with Anti-p57kip2 (ABT214) Antibody

Anti-CDKN1C antibody (200-316 aa) [ABT214] (STJ197275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Human partial mole stained with anti-p57 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of cytotrophoblasts in partial mole.

Anti-CDKN1C antibody [KP10] (STJ180208)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £528.50

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Whole cell lysates of Hela were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p16 (PTR2301) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 16kDaObserved band size: 16kDa

Anti-CDKN2A antibody (100-156 aa) [PTR2301] (STJ196735)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-CDKN2A antibody (57-156) [S3MR] (STJ113253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various cells using p14 Polyclonal Antibody diluted at 1：500

Anti-CDKN2A antibody (71-120 aa) (STJ94855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry of baboon lung with Anti-CDKN2A primary antibody (STJ502020) at 1:100 dilution in buffer. Section was treated with DAB and Haematoxylin stain.

Anti-CDKN2A antibody (N-Term) (STJ502020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CDKN2A antibody (N-Term) {Biotin} (STJ502023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CDKN2A antibody (N-Term) {FITC} (STJ502024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Western blot analysis using p16 monoclonal antibody against truncated p16 recombinant protein.

Anti-CDKN2A antibody [1E12E10] (STJ98296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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