Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using GRK2 (Phospho-Ser29) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-GRK2-Ser29 antibody (14-63 aa) (STJ90813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-GRK2-Tyr86 antibody (STJ193211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-GSK3 alpha and beta-Tyr216/Tyr279 antibody [R47-6P-9] (STJA0035670)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-GSK3 Alpha/Beta-Tyr216/279 antibody [PT0733R] (STJA0039234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of Hela cell. 1, GSK3 Alpha/Beta (phospho Tyr279/216) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-GSK3 Alpha/Beta-Tyr279/216 antibody (246-295 aa) (STJ90283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-GSK3 Beta-Ser9 antibody [PT0078R] (STJA0039695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Anti-Phospho-GSK3 beta-Ser9 antibody [R06-6H5] (STJA0033924)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various cells using Phospho-GSK3 Alpha (S21) Polyclonal Antibody

Anti-Phospho-GSK3A-Ser21 antibody (10-59 aa) (STJ90282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells using Phospho-GSK3 Beta-S9 Rabbit pAb (STJ22160) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-GSK3B-S9 antibody (STJ22160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of rat-spleen tissue. 1, GSK3 Beta (phospho Ser9) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-GSK3B-Ser9 antibody (1-50 aa) (STJ90284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-GSK3 Beta (S9) Polyclonal Antibody diluted at 1：500

Anti-Phospho-GSK3B-Ser9 antibody (STJ90445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Glycogen Synthase 1 (S645) Polyclonal Antibody

Anti-Phospho-GYS1-Ser645 antibody (611-660 aa) (STJ90793)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-H2AX-Ser139 antibody (94-143 aa) (STJ90288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Hamster

£49.00 - £261.00

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

Anti-Phospho-H2AX-Ser139 antibody [2A9-B5] (STJ99251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£149.00 - £360.00

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Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-H3-3A/H3-3B-Ser31 antibody (16-65 aa) (STJ91330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-HAND1-Ser98 antibody (71-120 aa) (STJ91158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of A549 cells using Phospho-HBP1 (S402) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-HBP1-Ser402 antibody (371-420 aa) (STJ90526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HCK-Tyr410 antibody (381-430 aa) (STJ91341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using HDAC1 (Phospho-Ser421) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC1-Ser421 antibody (387-436 aa) (STJ90497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HDAC3-Ser424 antibody (379-428 aa) (STJ91081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HDAC5 (S498) Polyclonal Antibody diluted at 1：500

Anti-Phospho-HDAC5-Ser498 antibody (464-513 aa) (STJ90286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-HDAC5-Ser498 antibody [PT1439R] (STJA0040328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of HepG2 cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-HDAC5/9-Ser259/220 antibody (225-274 aa) (STJ90459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, HDAC6 (phospho Ser22) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-HDAC6-Ser22 antibody (7-56 aa) (STJ91082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry of paraffin-embedded human lung carcinoma using Phospho-HDAC8-S39 antibody (STJ22168).

Anti-Phospho-HDAC8-S39 antibody (STJ22168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-HDAC8 (S39) Polyclonal Antibody diluted at 1：500

Anti-Phospho-HDAC8-Ser39 antibody (5-54 aa) (STJ90287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-HER2-Tyr1221/1222 antibody [PT0740R] (STJA0039238)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA/CHIP/Cut&Tag

Reactivity :

Human

£150.00 - £540.00

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Anti-Phospho-HER2/HER4-Tyr1284 antibody [PT1236R] (STJA0040156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HGS-Tyr334 antibody (301-350 aa) (STJ91095)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-HIRA-Thr555 antibody (521-570 aa) (STJ91346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Histone H1 (phospho Thr17) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-Histone H1-Thr17 antibody (1-50 aa) (STJ91085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Anti-Phospho-Histone H1.4-Thr17 antibody [R07-3C1] (STJA0035939)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (STJ27513) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-Histone H2A-S139 antibody (STJ27513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of Hela+UV 5' cells using Phospho-Histone H2A (T121) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Histone H2A-Thr121 antibody (81-130 aa) (STJ91231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Histone H2A.X-Ser139 antibody [2A9-B5] (STJA0033230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-Histone H2A.X-Ser139 antibody [7G9-H4] (STJA0033231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-Histone H2A.X-Ser139 antibody [PT0330R] (STJA0039611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-Histone H2A.X-Ser139 antibody [R03-2Z1] (STJA0036230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Histone H2AX-Ser139 antibody [C6] (STJA0019078)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF

£195.50 - £1,319.50

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Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit pAb (STJ11100981) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10 antibody (STJ11100981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Phospho-Histone H3-S10 antibody [SRM13680] (STJ11108410)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

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Western blot analysis of various lysates using Phospho-Histone H3-S10/T11 Rabbit pAb (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10/T11 antibody (STJ11101033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (STJ11100980) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S28 antibody (STJ11100980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Histone H3 (phospho Ser10) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-Histone H3-Ser10 antibody (1-50 aa) (STJ90289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IHC-F/FC/ICC/IF/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Anti-Phospho-Histone H3-Ser10 antibody [R06-6O9] (STJA0035817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Histone H3-Ser10/Thr11 antibody [PT0977R] (STJA0039792)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-Histone H3-Ser10/Thr11 antibody [R08-6C4] (STJA0035070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options