• Western blot analysis of extracts of various cell lines, using Alpha-tubulin (ubiquitous) chain antibody (STJ11103689) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (STJ11103689) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (STJ11103689) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain antibody (STJ11103689) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain antibody (STJ11103689) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Alpha-tubulin (ubiquitous) chain antibody (STJ11103689) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Alpha-tubulin antibody (1-80) (STJ11103689)

SKU:
STJ11103689

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alpha-tubulin (1-80) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBA1B
Gene ID: 10376
Uniprot ID: TBA1B_HUMAN
Immunogen Region: 1-80
Immunogen: Recombinant fusion protein containing a sequence within amino acids 1-80 of human Alpha-tubulin (ubiquitous) chain (NP_006073.2).
Immunogen Sequence: MRECISIHVGQAGVQIGNAC WELYCLEHGIQPDGQMPSDK TIGGGDDSFNTFFSETGAGK HVPRAVFVDLEPTVIDEVRT
Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution. Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (KIAA0895L/MATCAP, VASH1 or VASH2) and tubulin tyrosine ligase (TTL), respectively. Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1. Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules. In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport. Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator. Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction.
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name Tubulin Alpha-1b Chain
Alpha-Tubulin Ubiquitous
Tubulin K-Alpha-1
Tubulin Alpha-Ubiquitous Chain Cleaved Into - Detyrosinated Tubulin Alpha-1b Chain
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-190840
Reactome: R-HSA-190861
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-3371497
Reactome: R-HSA-380320
Reactome: R-HSA-389960
Reactome: R-HSA-389977
Reactome: R-HSA-437239
Reactome: R-HSA-5610787
Reactome: R-HSA-5617833
Reactome: R-HSA-5620924
Reactome: R-HSA-5626467
Reactome: R-HSA-5663220
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811436
Reactome: R-HSA-68877
Reactome: R-HSA-8852276
Reactome: R-HSA-8955332
Reactome: R-HSA-9013407
Reactome: R-HSA-9609690
Reactome: R-HSA-9609736
Reactome: R-HSA-9619483
Reactome: R-HSA-9646399
Reactome: R-HSA-9648025
Reactome: R-HSA-9668328
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tubulin Alpha-1b Chain antibody
Anti-Alpha-Tubulin Ubiquitous antibody
Anti-Tubulin K-Alpha-1 antibody
Anti-Tubulin Alpha-Ubiquitous Chain Cleaved Into - Detyrosinated Tubulin Alpha-1b Chain antibody
Anti-TUBA1B antibody

Information sourced from Uniprot.org

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