Immunofluorescence Antibodies | St John's Laboratory

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros antibody (STJ24155) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IKZF1 antibody (1-270) (STJ24155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Ikaros rabbit monoclonal antibody (STJ11101900) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Ikaros in representative gene loci (MICA) , as shown in figure.

Anti-IKZF1 antibody (250-350) [S0MR] (STJ11101900)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse

£146.50 - £642.50

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Immunofluorescence analysis of MCF-7 cells using IKZF3 antibody (STJ29426). Blue: DAPI for nuclear staining.

Anti-IKZF3 antibody (280-450) (STJ29426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from Raji cells, using IKZF3 Rabbit mAb (STJ11103626) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-IKZF3 antibody (400-509) [S6MR] (STJ11103626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ72126 (1ug/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IKZF4 antibody (Internal) (STJ72126)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Dog/Cow

£312.50

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Anti-IL-1 beta antibody [SM0422] (STJA0010422)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunohistochemistry analysis of paraffin-embedded rat lung using IL-10 at dilution of 1:2000.

Anti-IL-10 antibody [SM4919] (STJA0004919)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

£170.50 - £233.50

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Anti-IL-17A antibody [HAA10E10] (STJ16102485)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/IF

Reactivity :

Human

£890.50

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Anti-IL-17A antibody [HAA10E10] {Alexa-fluor-488} (STJ16102482)

Clonality :

Monoclonal

Host :

Human

Applications :

IF

Reactivity :

Human

£1,095.50

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Confocal imaging of 293F cells transfected with IL-2RB using IL-2RB Rabbit mAb (STJ11107355, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-IL-2RB antibody (27-235 aa) [SRM] (STJ11107355)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Anti-IL-33R antibody (27-332 aa) [SRM] (STJ11107190)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Confocal imaging of HeLa cells using IL10 Rabbit monoclonal antibody (STJ11102745, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-IL10 antibody (1-100) [S5MR] (STJ11102745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of THP-1, Romas, HepG2, SW480, SHSY5Y, Mouse liver cells using IL-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL10 antibody (71-120 aa) (STJ96503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using IL10RA Rabbit polyclonal antibody (STJ24158) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL10RA antibody (265-578) (STJ24158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using IL-10R Alpha Polyclonal Antibody

Anti-IL10RA antibody (462-511 aa) (STJ93673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using IL10RB Rabbit polyclonal antibody (STJ118126) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL10RB antibody (20-220) (STJ118126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using IL11 antibody (STJ29870) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL11 antibody (22-199) (STJ29870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of IL-11 antibody in paraffin-embedded human pancreas tissue.

Anti-IL11 antibody (27-76 aa) (STJ93674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa cells using IL-12A Polyclonal Antibody

Anti-IL12A antibody (16-65 aa) (STJ93676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-IL12A antibody (23-219 aa) (STJ11107225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of MCF7 cells using IL-12A p35 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL12A antibody (31-80 aa) (STJ96807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of mouse-kidney lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-IL12B antibody (241-290 aa) (STJ98797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using IL-13R Alpha 1 Polyclonal Antibody diluted at 1：1000

Anti-IL13RA1 antibody (371-420 aa) (STJ93678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HepG2 (UV), L929 cells using IL-13R Alpha 2 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-IL13RA2 antibody (241-290 aa) (STJ97311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using IL13RA2 Rabbit polyclonal antibody (STJ11103023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL13RA2 antibody (50-150) (STJ11103023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer, antibody was diluted at 1:100

Anti-IL15 antibody (111-160 aa) (STJ99006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using CD215/IL-15R alpha Rabbit polyclonal antibody (STJ11103511) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL15RA antibody (167-267) (STJ11103511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293, HepG2, BXPC-3, Raji, mouse lung, mouse brain, mouse spleen cells using IL-16 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL16 antibody (1281-1330 aa) (STJ96540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using IL17A antibody (STJ114328) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IL17A antibody (1-100) (STJ114328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200

Anti-IL17B antibody (1-50 aa) (STJ98796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of A549, using IL17C rabbit polyclonal antibody (STJ112601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL17C antibody (19-197) (STJ112601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Anti-IL17C antibody (81-130 aa) (STJ98799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-IL17D antibody (141-190 aa) (STJ99011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer, antibody was diluted at 1:100

Anti-IL17F antibody (81-130 aa) (STJ99010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot with Anti-IL17RA Antibody (STJ501422) with peptide at 1:750 dilution in buffer. 12% gel.

Anti-IL17RA antibody (110-150) (STJ501422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-IL17RA antibody (110-150) {Biotin} (STJ501423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-IL17RA antibody (110-150) {FITC} (STJ501424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-IL18 antibody (1-193 aa) [SRM] (STJ11106987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ71925 (0. 1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IL18 antibody (Internal) (STJ71925)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IL18 antibody (STJ192030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using IL18BP antibody (STJ28528). Blue: DAPI for nuclear staining.

Anti-IL18BP antibody (31-194) (STJ28528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of NCI-H460 cells, using IL18R1/IL-18 R alpha antibody (STJ24172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL18R1 antibody (19-320) (STJ24172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Anti-IL18RAP antibody (291-340 aa) (STJ98755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293T Hela VEC KB mouse-kidney lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-IL19 antibody (51-100 aa) (STJ98773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-IL1A antibody (8-57 aa) (STJ93686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of THP-1 cells using IL1 Beta Rabbit polyclonal antibody (STJ113778) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL1B antibody (1-269) (STJ113778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ24174) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-IL1B antibody (1-269) (STJ24174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of THP-1 cells, using IL1 Beta antibody (STJ11101739) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IL1B antibody (117-269) [S9MR] (STJ11101739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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