• STJ73648 (0. 1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73648 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73648 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73648 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ73648 (3. 75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-EHD1 antibody (N-Term) (STJ73648)

SKU:
STJ73648-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC/FC
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EHD1 (N-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EHD1
Gene ID: 10938
Uniprot ID: EHD1_HUMAN
Immunogen Region: N-Term
Accession Number: NP_001269373.1; NP_001269374.1
Immunogen Sequence: FSWVSKDARRKKEPC
Function ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis.
Protein Name Eh Domain-Containing Protein 1
Past Homolog 1
Hpast1
Testilin
Database Links Reactome: R-HSA-983231
Cellular Localisation Recycling Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Early Endosome Membrane
Cell Membrane
Cell Projection
Cilium Membrane
Preferentially Associates With Tubular Recycling Endosomes
Colocalizes With Fer1l5 At Plasma Membrane In Myoblasts And Myotubes
Localizes To The Ciliary Pocket From Where The Cilium Protrudes
Colocalizes With Bace1 In Tubulovesicular Cytoplasmic Membranes
Colocalizes With Bace1 And App Amyloid Beta Proteins In Hippocampal Mossy Fiber Terminals
Alternative Antibody Names Anti-Eh Domain-Containing Protein 1 antibody
Anti-Past Homolog 1 antibody
Anti-Hpast1 antibody
Anti-Testilin antibody
Anti-EHD1 antibody
Anti-PAST antibody
Anti-PAST1 antibody
Anti-CDABP0131 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance