Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (STJ11103707) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-His-Tag antibody (STJ11103707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP

Reactivity :

Species independent

$97.88 - $405.68

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Western blot analysis of lysates from His Protein, using Rabbit anti His-tag mAb (STJ11103712) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-His-Tag antibody [S2MR] (STJ11103712)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

$280.13 - $529.88

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Western blot analysis of various lysates, using His-Tag mAb (STJ11103695) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-His-Tag antibody [S5MM] (STJ11103695)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

$80.33 - $336.83

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Anti-His-Tag antibody [SMM] (STJ11107566)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

$182.93 - $336.83

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Anti-His-tag antibody [SRM] (STJ11107582)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

$182.93 - $336.83

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Western blot analysis of various lysates using HIST1H2BG Rabbit pAb (STJ11107549) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HIST1H2BG antibody (1-126 aa) (STJ11107549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using Histone H1t antibody (STJ11100542) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Histone H1 antibody (110-209) (STJ11100542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Confocal imaging of U-2 OS cells using Histone H1. 2 Rabbit monoclonal antibody (STJ11102702, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-Histone H1.2 antibody (1-100) [S2MR] (STJ11102702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $775.58

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Immunocytochemistry analysis of Histone H1. 3 (green) in Hela using Histone H1. 3 antibody, and DAPI (blue)

Anti-Histone H1.3 antibody (5-17 aa) [R09-8H7] (STJA0011419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$332.78

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Anti-Histone H3 antibody (36-136) (STJ23980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using Histone H4 Rabbit polyclonal antibody (STJ119273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Histone H4 antibody (1-100) (STJ119273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$151.88 - $708.08

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Immunocytochemistry analysis of Histone H4 (green) in Hela using Histone H4 antibody, and DAPI (blue).

Anti-Histone H4 antibody (51-70 aa) [R05-8H9] (STJA0011428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/ChIP

Reactivity :

Human

$332.78

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Anti-HIV p24 antibody [ND1] (STJ16100518)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/ELISA

Reactivity :

HIV

$655.43

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Anti-HIV-1 Nef antibody (163-173 aa) [3E6] (STJA0002834)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/RIP

Reactivity :

HIV-1

$591.98 - $3,378.38

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Anti-HIV-1 Nef antibody (35-50 aa) [3D12] (STJA0002833)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

HIV-1

$591.98 - $3,378.38

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g HK1 antibody (STJ24019). Western blot was performed from the immunoprecipitate using HK1 antibody (STJ24019) at a dilution of 1:500.

Anti-HK1 antibody (21-219) (STJ24019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.

Anti-HK1 antibody (50-150) [S9MR] (STJ11101239)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of U2OS cells using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HK2 antibody (1-120) (STJ24020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using HK3 Rabbit pAb (STJ110726) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HK3 antibody (1-270) (STJ110726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-HLA-A, B, C antibody [W6/32] (STJA0027020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/ICC/IHC-F/IP/WB

Reactivity :

Human

$343.58 - $510.98

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Western blot analysis of various lysates using MHC class I (HLA-A/B) Rabbit mAb (STJ11102369) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-HLA-A/HLA-B antibody (200-300) [S9MR] (STJ11102369)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$197.78 - $867.38

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Immunohistochemistry analysis of HLA-B in paraffin-embedded mouse spleen using HLA-B Rabbit polyclonal antibody (STJ24022) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HLA-B antibody (1-100) (STJ24022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using HLA-C antibody (STJ111053) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HLA-C antibody (25-308) (STJ111053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-HLA-Class I antibody [W6/32] (STJ16100127)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-F/ICC/ELISA

Reactivity :

Cow/Non-Human Primates/Human/Cat

$690.53

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Anti-HLA-Class I antibody [W6/32] {Biotin} (STJ16100128)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-F/ICC/ELISA

Reactivity :

Cow/Non-Human Primates/Human/Cat

$1,013.18

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Confocal imaging of paraffin-embedded Mouse spleen tissue using HLA-DPB1 Rabbit mAb (STJ11107363, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-HLA-DPB1 antibody (1-100 aa) [SRM] (STJ11107363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of Raji cells using HLA-DPB1 Rabbit polyclonal antibody (STJ115276) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HLA-DPB1 antibody (40-100) (STJ115276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 Rabbit polyclonal antibody (STJ119591) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HLA-DPB1 antibody (40-100) (STJ119591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-HLA-DR antibody (100-200 aa) [SRM] (STJ11107240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

$197.78 - $867.38

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Anti-HLA-G antibody [MEM-G/9] (STJ16100196)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/IHC-F/ICC/ELISA

Reactivity :

Human

$776.93

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Anti-HLA-G antibody [MEM-G/9] {Biotin} (STJ16100197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/IHC-F/ICC/ELISA

Reactivity :

Human

$1,146.83

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Immunofluorescence analysis of A-549 cells using HMG20A antibody (STJ29425).

Anti-HMG20A antibody (1-347) (STJ29425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using HMGA1 antibody (STJ11102046) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-HMGA1 antibody (1-107) [S6MR] (STJ11102046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1 Rabbit polyclonal antibody (STJ11105655) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGB1 antibody (1-215) (STJ11105655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (STJ11101678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HMGB1 antibody (100-200) [S8MR] (STJ11101678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g HMGB2 Rabbit mAb (STJ11102444) , along with a Goat Anti-Rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1) , as shown in figure.

Anti-HMGB2 antibody (1-100) [S4MR] (STJ11102444)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various lysates using HMGB2 Rabbit polyclonal antibody (STJ119486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMGB2 antibody (100-200) (STJ119486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded Mouse liver tissue using HMGCR Rabbit polyclonal antibody (STJ11105656) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HMGCR antibody (200-300) (STJ11105656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using HMGCR Rabbit polyclonal antibody (STJ119212) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGCR antibody (351-440) (STJ119212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded Human liver tissue using HMGCR Rabbit polyclonal antibody (STJ115278) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HMGCR antibody (629-888) (STJ115278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using (STJ119211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HMGCR antibody (783-791) (STJ119211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of Rat brain, using HMGCR rabbit polyclonal antibody (STJ24046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HMGCR antibody (783-791) (STJ24046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using HMGCS1 Rabbit polyclonal antibody (STJ24047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGCS1 antibody (241-520) (STJ24047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using HMGCS2 rabbit polyclonal antibody (STJ116457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMGCS2 antibody (269-508) (STJ116457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-HMGN1 antibody (1-100 aa) (STJ11106363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using HMGN1 antibody (STJ27908) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HMGN1 antibody (1-100) (STJ27908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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