Anti-Cytokeratin 18 antibody (79-430aa) [AT2G3] (STJA0041597)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2bk
ImmunogenRecombinant human KRT18 (79-430aa) purified from E. coli
General Information
| Short Description | Mouse monoclonal anti-Cytokeratin 18 (79-430aa) for use in ELISA, WB, ICC, IF and IHC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IF/IHC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | AT2G3 |
| Isotype | IgG2bk |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | By protein-G affinity chromatography |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KRT18 |
| Gene ID | 3875 |
| Uniprot ID | K1C18_HUMAN |
| Accession Number | NP_000215 |
| Immunogen | Recombinant human KRT18 (79-430aa) purified from E. coli |
| Immunogen Region | 79-430aa |
Additional Info
| Tissue Specificity | Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. |
| Post Translational Modifications | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
| Function | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Protein Name | Keratin - Type I Cytoskeletal 18Cell Proliferation-Inducing Gene 46 ProteinCytokeratin-18Ck-18Keratin-18K18 |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | Nucleus MatrixCytoplasmPerinuclear RegionNucleusNucleolus |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 18 antibodyAnti-Cell Proliferation-Inducing Gene 46 Protein antibodyAnti-Cytokeratin-18 antibodyAnti-Ck-18 antibodyAnti-Keratin-18 antibodyAnti-K18 antibodyAnti-KRT18 antibodyAnti-CYK18 antibodyAnti-PIG46 antibody |
Information sourced from Uniprot.org