Anti-Cytokeratin 20 antibody [R02-9F4] (STJA0035887)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Cytokeratin 20
General Information
| Short Description | Rabbit monoclonal anti-Cytokeratin 20 for use in WB, IHC-P, ICC, IF, IP and FC in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-9F4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:30FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | KRT20 |
| Gene ID | 54474 |
| Uniprot ID | K1C20_HUMAN |
| Immunogen | A synthesized peptide derived from human Cytokeratin 20 |
Additional Info
| Tissue Specificity | Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa.specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. |
| Post Translational Modifications | Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. |
| Function | Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. |
| Protein Name | Keratin - Type I Cytoskeletal 20Cytokeratin-20Ck-20Keratin-20K20Protein It |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 20 antibodyAnti-Cytokeratin-20 antibodyAnti-Ck-20 antibodyAnti-Keratin-20 antibodyAnti-K20 antibodyAnti-Protein It antibodyAnti-KRT20 antibody |
Information sourced from Uniprot.org