ELISA Antibodies | St John's Laboratory

Immunofluorescence analysis of U2OS cells using PKR/PKR/EIF2AK2 antibody (STJ11105689) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EIF2AK2 antibody (1-270) (STJ11105689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates, using PKR/EIF2AK2 Rabbit polyclonal antibody (STJ27547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.

Anti-EIF2AK2 antibody (1-270) (STJ27547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded Mouse kidney tissue using PKR Rabbit polyclonal antibody (STJ11105620) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-EIF2AK2 antibody (350-551) (STJ11105620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PKR Polyclonal Antibody diluted at 1：2000

Anti-EIF2AK2 antibody (413-462 aa) (STJ95144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PKR/EIF2AK2 antibody (STJ11101690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK2 antibody (450-551) [S0MR] (STJ11101690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-Eif2ak2 antibody (STJ115544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry of rat tissue with Anti-EIF2AK3 primary antibody (STJ502417) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-EIF2AK3 antibody (1000-1050) (STJ502417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Western blot with Anti-EIF2AK3 Antibody (STJ502418) on rat adrenal cells. Left) Chicken serum antibodies, Right) Total PERK IgY.

Anti-EIF2AK3 antibody (1000-1050) (STJ502418)

Clonality :

Polyclonal

Host :

Chicken

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-EIF2AK3 antibody (1000-1050) {Biotin} (STJ502419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EIF2AK3 antibody (1000-1050) {Biotin} (STJ502421)

Clonality :

Polyclonal

Host :

Chicken

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EIF2AK3 antibody (1000-1050) {FITC} (STJ502420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EIF2AK3 antibody (1000-1050) {FITC} (STJ502422)

Clonality :

Polyclonal

Host :

Chicken

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of MCF7 cells using PERK Rabbit polyclonal antibody (STJ11105581) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (50-600) (STJ11105581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PERK Rabbit polyclonal antibody (STJ11100155) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (867-1116) (STJ11100155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, PERK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-EIF2AK3 antibody (947-996 aa) (STJ95031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-EIF2AK3 antibody (STJ99624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using PERK monoclonal antibody against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.

Anti-EIF2AK3 antibody [5G5] (STJ98319)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts of HepG2 cells, using GCN2 Antibody (STJ114492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK4 antibody (1-300) (STJ114492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using GCN2 Rabbit polyclonal antibody (STJ29235) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF2AK4 antibody (1-300) (STJ29235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using GCN2 rabbit monoclonal antibody (STJ11101809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK4 antibody (1-300) [S9MR] (STJ11101809)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry of rat tissue with Anti-EIF2AK4 primary antibody (STJ501136) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-EIF2AK4 antibody (1600-1700) (STJ501136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Western blot with Anti-EIF2AK4 Antibody (STJ501137) of kidney at 1:500 dilution in buffer.

Anti-EIF2AK4 antibody (1600-1700) (STJ501137)

Clonality :

Polyclonal

Host :

Chicken

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-EIF2AK4 antibody (1600-1700) {Biotin} (STJ501138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EIF2AK4 antibody (1600-1700) {Biotin} (STJ501140)

Clonality :

Polyclonal

Host :

Chicken

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EIF2AK4 antibody (1600-1700) {FITC} (STJ501139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-EIF2AK4 antibody (1600-1700) {FITC} (STJ501141)

Clonality :

Polyclonal

Host :

Chicken

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-EIF2AK4 antibody (865-914 aa) (STJ93239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using GCN2 Rabbit monoclonal antibody (STJ11105120) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-EIF2AK4 antibody [S5120RM] (STJ11105120)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates using EIF2B1 Rabbit pAb (STJ110201) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-EIF2B1 antibody (1-305) (STJ110201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2B2 antibody (1-120) (STJ29107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF2B2 antibody (50-130 aa) (STJ191550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using EIF2B3 antibody (STJ112300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B3 antibody (1-270) (STJ112300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using eIF2B Gamma Polyclonal Antibody diluted at 1：1000

Anti-EIF2B3 antibody (240-320 aa) (STJ92870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of EIF2B4 Antibody. The lane on the right is blocked with the EIF2B4 peptide.

Anti-EIF2B4 antibody (226-275 aa) (STJ92871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using EIF2B4 Rabbit polyclonal antibody (STJ11100161) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2B4 antibody (30-100) (STJ11100161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) (EIF2B5) Rabbit mAb (STJ11102350) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-EIF2B5 antibody (1-100) [S0MR] (STJ11102350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301). Western blot was performed from the immunoprecipitate using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301) at a dilution of 1:1000.

Anti-EIF2B5 antibody (442-721) (STJ112301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-EIF2B5 antibody (480-560 aa) (STJ190118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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HEK293 overexpressing EIF2C1 and probed with STJ70872 (non-transfected HEK293 in lane B).

Anti-EIF2C1/AGO1 antibody (Internal) (STJ70872)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using eIF2C3 Rabbit monoclonal antibody (STJ11103159) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-eIF2C3 antibody (721-860) [S9MR] (STJ11103159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using eIF2 Alpha antibody (STJ23509) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EIF2S1 antibody (1-260) (STJ23509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-EIF2S1 antibody (21-70 aa) (STJ92872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Fish

£48.00 - £252.00

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Western blot analysis of various lysates using EIF2S2 Rabbit polyclonal antibody (STJ11102835) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EIF2S2 antibody (1-175) (STJ11102835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF2S2 antibody (10-90 aa) (STJ92873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using EIF2S2 Rabbit pAb (STJ28167) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-EIF2S2 antibody (134-333) (STJ28167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of the lysates from HT-29 cells using EIF2S2 antibody.

Anti-EIF2S2 antibody (61-110 aa) (STJ92874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S3 antibody (STJ28664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EIF2S3 antibody (1-250) (STJ28664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of rat brain, using EIF2S3 antibody (STJ23510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2S3 antibody (173-472) (STJ23510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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