Nanobodies

Single-domain access to hard-to-reach epitopes.

Nanobodies are monoclonal, alpaca-derived single-domain antibodies supplied with defined clone IDs, targeting proteins across human, mouse, rat, and other species.

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How it works

A single variable domain, not a full IgG

Nanobodies (VHH antibodies) are the variable domains of the heavy-chain-only antibodies naturally found in camelids such as alpaca. Their compact single-domain structure, approximately 15 kDa in molecular weight, provides high thermal stability and solubility. This small size allows nanobodies to access epitopes within clefts, active sites, and conformationally restricted regions on target proteins that are inaccessible to conventional IgG antibodies.

Category benefits

Why use nanobodies?

✓	Access to hidden epitopes The compact single-domain structure allows nanobodies to reach epitopes within clefts, active sites, and conformationally restricted regions that conventional IgG antibodies cannot access.
✓	High thermal stability and solubility At approximately 15 kDa, the single-domain structure gives nanobodies stability and solubility characteristics distinct from full-length antibodies.
✓	Defined, monoclonal identity All nanobodies in the range are monoclonal and supplied with defined clone IDs, giving a consistent, traceable reagent for repeat experiments.
✓	Multi-species reactivity The range targets proteins across human, mouse, rat, and other species, supporting cross-species study designs from a single clone family.

Where they're used

Typical applications

✓	Detection assays (ELISA, Western Blot) Used to detect and quantify target proteins in solution-based and blot-based formats.
✓	Cellular analysis (Flow Cytometry, Immunofluorescence) Used to localise or quantify target proteins on and within cells.
✓	Immunoprecipitation Used to pull down a target protein and any bound complexes from a sample.
✓	Functional Blocking and Neutralisation Used where access to a target's active site or a conformationally restricted region is required to block or neutralise its function.

Choosing a nanobody for a specific target

The technical support team is available to help match a nanobody to a given target. Antibodies are dispatched on overnight priority shipping with gel ice packs.

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