Positive Controls

Peptides

Positive Control Peptides

Confirm your assay is working before you trust a negative result

Positive control peptides carry the same sequence as the immunogen used to raise a specific antibody. A successful signal confirms the antibody can recognise its intended epitope, and that your ELISA, Western blot, or IHC assay is functioning correctly.

How it works

Immunogen-sequence validation of antibody binding

A positive control peptide contains the same sequence as the immunogen originally used to raise its antibody. In a direct ELISA format, the peptide is coated onto the plate. A successful binding signal confirms the antibody can recognise and bind that specific epitope, independent of whatever experimental sample is being tested alongside it.

Category benefits

Why use positive control peptides?

✓ Direct confirmation of antibody binding
Confirm your antibody binds its intended target before you run it against experimental samples.
✓ Reliable data interpretation
A successful signal from a positive control peptide gives definitive evidence that your assay can detect the antigen of interest.
✓ Trustworthy negative results
A working positive control confirms the whole procedure is functioning, so a negative result elsewhere in the experiment is genuine rather than a sign of assay failure.
✓ Support for assay optimisation
Use as a known benchmark when developing or troubleshooting a new antibody or assay protocol.
Where they're used

Typical applications

✓ Direct ELISA
Coat the plate with the positive control peptide to confirm antibody binding and epitope recognition directly.
✓ Antibody development
Benchmark new antibody batches or lots against a known-good positive control before releasing them for use.
✓ Assay optimisation
Use as a working benchmark when troubleshooting or optimising a new ELISA, Western blot, or IHC protocol.
✓ Western blot and IHC validation
Run alongside experimental samples to confirm the assay procedure and detection system are working before drawing conclusions from the results.

Choosing the right positive control

Positive controls need to match the immunogen used to raise your specific antibody. Contact our team for help finding the correct match from our catalogue.

Looking for more validation tools?

Browse our full range of positive control peptides for antibody and assay validation.

 View positive control insights

For Research Use Only. Not for use in diagnostic procedures.

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