Secondary Antibodies | St John's Laboratory

Immunofluorescence analysis of PC-12 cells using Vimentin Rabbit mAb (STJ1161657) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (STJS001188) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Donkey Anti-Rabbit IgG H+L antibody {FITC} (STJS001188)

Clonality :

Polyclonal

Host :

Donkey

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£67.50 - £112.50

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Western blot analysis of lysates from HeLa cells, using Beta-actin antibody as the primary antibody. <br/>Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (STJS001185) at 1:2000-1:30000 dilution<br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Donkey Anti-Rabbit IgG H+L antibody {HRP} (STJS001185)

Clonality :

Polyclonal

Host :

Donkey

Applications :

WB/IHC-P

Reactivity :

Rabbit

£51.50 - £79.50

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Immunofluorescence analysis of HeLa cells, using PARP1 antibody (STJ29827) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. <br/>Secondary antibody: ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (STJS001183) at 1:100 dilution. <br/>Blue: DAPI for nuclear staining.

Donkey Anti-Rabbit IgG H+L antibody {ABflo® 488} (STJS001183)

Clonality :

Polyclonal

Host :

Donkey

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£88.50 - £152.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 647} (STJS000846)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG (H&L) antibody {Alexa Fluor 633} (STJS000836)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 594} (STJS000827)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 568} (STJS000818)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 488} (STJS000809)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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Donkey Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 555} (STJS000800)

Clonality :

Polyclonal

Host :

Donkey

Reactivity :

Rabbit

£208.50

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