St John's Laboratory Ltd

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cyclin D1-T286 pAb (STJ11101099) at 1:1000 dilution or Cyclin D1 antibody (STJ293820). NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CCND1-T286 antibody (STJ11101099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Cyclin D1 Blocking Peptide peptide (STJ503889)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Cyclin D1 Blocking Peptide peptide (STJ503890)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Smad4 Blocking Peptide peptide (STJ504943)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Smad4 Blocking Peptide peptide (STJ504945)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit monoclonal antibody (STJ11101576, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-CCND1 antibody (196-295) [S6MR] (STJ11101576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Cyclin D1 Blocking Peptide peptide (STJ503888)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Anti-Cyclin D1 antibody (STJA0037764)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

€293.40 - €402.60

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Smad4 Blocking Peptide peptide (STJ504942)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Smad4 Antibody Positve Control Positive Control peptide (STJ505161)

Applications :

WB

€370.20

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Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (STJ11102750) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Cyclin D1 antibody (200-295) (STJ11102750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CCND1-Ser90 antibody (STJA0009828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Human PDPK1 (Phosphoinositide Dependent Protein Kinase 1) Sandwich ELISA Kit (STJE0011386)

Applications :

ELISA

Reactivity :

Human

€505.80 - €703.80

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Western blot A431 cells anti-PDK1 antibody reactivity with pervanadate treatment STJA0041332

Anti-PDK1 antibody (N-Term) (STJA0041332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€569.40

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Western blot A431 cells probed with anti-PDK1 Tyr-9 rabbit polyclonal antibody STJA0041333

Anti-Phospho-PDK1-Tyr-9 antibody (STJA0041333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€651.00

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PDPK1 antibody western blot HEK293 lysates STJA0036128

Anti-PDPK1 antibody [R05-9F7] (STJA0036128)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (STJ29323) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PDPK1-S241 antibody (STJ29323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Human PDPK1 (Phosphoinositide Dependent Protein Kinase 1) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017772)

Applications :

ELISA

Reactivity :

Human

€528.60 - €737.40

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Anti-PDK1 antibody (C-Term) [M146] (STJA0005520)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€383.40

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Western blot analysis of extracts of various cell lines, using PDK1/PDPK1 antibody (STJ11104559) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PDPK1 antibody (409-556) (STJ11104559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Anti-Recombinant-PDK1/PDPK1 antibody [RM1D89] (STJA0022984)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€360.60 - €549.00

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0041348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Cyclin D1 antibody IHC Human mantle cell lymphoma STJ180503

Anti-Recombinant-Cyclin D1 antibody (200-295aa) [ZM178] (STJ180503)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

€175.80 - €669.00

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Cyclin D1 Blocking Peptide peptide (STJ503892)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Cyclin D1 Blocking Peptide peptide (STJ503893)

Applications :

Immunodepletion/Immunocompetition

€312.60

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Olig2 Positive Control peptide (STJ504497)

Applications :

WB

€370.20

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Cyclin D1 antibody western blot NIH 3T3 C6 lysates STJA0035915

Anti-Cyclin D1 antibody [R09-7J5] (STJA0035915)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Immunohistochemistry analysis of paraffin-embedded rat brain using PDPK1 antibody (STJ24950) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDPK1 antibody (409-556) (STJ24950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF, using PDK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PDPK1 antibody (210-259 aa) (STJ95010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Mouse PDPK1 (Phosphoinositide Dependent Protein Kinase 1) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017773)

Applications :

ELISA

Reactivity :

Mouse

€541.80 - €755.40

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Anti-Phospho-PDK1-Tyr-9 antibody (STJA0005521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€441.00

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Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (STJ11105841) at1:2000 dilution. PC-3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-Phospho-PDPK1-S241 antibody [S5841RM] (STJ11105841)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Human PDK1/PDPK1 protein (Recombinant) (N-His) (STJP003253)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

€250.20 - €1,396.20

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Western blot mouse brain and human A431 cells probed with anti-PDK1 antibody STJA0041312

Anti-PDK1 antibody (C-Term) [M146] (STJA0041312)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

€569.40

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g Cyclin D1 antibody (STJ11101272). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (STJ11101272) at a dilution of 1:1000.

Anti-CCND1 antibody (200-295) (STJ11101272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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SDS-PAGE gel of recombinant human Smad4 protein under reducing conditions stained with Coomassie blue

Human Smad4 protein (Recombinant) (His-Tag) (STJP019762)

Host :

E.coli

Applications :

SDS-PAGE

€132.60 - €642.60

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Mouse PDPK1 (Phosphoinositide Dependent Protein Kinase 1) Sandwich ELISA Kit (STJE0011387)

Applications :

ELISA

Reactivity :

Mouse

€519.00 - €721.80

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Anti-Phospho-PDPK1-Ser241 antibody [PT0869R] (STJA0039151)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Anti-PDPK1 antibody [R03-7H6] (STJA0034703)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€168.60 - €370.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (STJ22329) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-PDPK1-S241 antibody (STJ22329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Ser241 antibody (210-259 aa) (STJ90382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using PDK1 (Phospho-Tyr9) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Tyr9 antibody (1-50 aa) (STJ91192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-PDK1 antibody (N-Term) (STJA0005519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€383.40

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0005522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€441.00

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Anti-PDPK1 antibody [PT0647R] (STJA0039247)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-CD105/Eng antibody [MJ7/18] {PE} (STJA0002240)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC

Reactivity :

Mouse

€165.00 - €274.20

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Anti-CD105/Eng antibody [MJ7/18] {FITC} (STJA0002565)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC

Reactivity :

Mouse

€165.00 - €274.20

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Recombinant Mouse ENG Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-75 kDa.

Mouse Endoglin/ENG/CD105 protein (Recombinant) (No-Tag) (STJP002987)

Host :

E.coli

€125.40 - €270.60

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