St John's Laboratory Ltd

Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITCH antibody (1-155) (STJ118061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using TIS11B Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ZFP36L1 antibody (58-107 aa) (STJ96033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ71108 (0. 1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ITCH/AIF4 antibody (Internal) (STJ71108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-ZFP36L1 antibody (200-250) {FITC} (STJ503575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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ITCH/AIP4 Blocking Peptide peptide (STJ504270)

Applications :

Immunodepletion/Immunocompetition

£260.50

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ZFP36L1 Blocking Peptide for STJ503573 peptide (STJ505474)

Applications :

Immunodepletion/Immunocompetition

£260.50

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SGK1 antibody western blot A431 cells STJA0035995

Anti-SGK1 antibody [R07-4G3] (STJA0035995)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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ITCH antibody western blot K562 cells STJA0037252

Anti-ITCH antibody [R05-6P2] (STJA0037252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using SGK1 Rabbit monoclonal antibody (STJ11101969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SGK1 antibody (1-100) [S9MR] (STJ11101969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using ITCH (Phospho-Tyr420) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ITCH-Tyr420 antibody (386-435 aa) (STJ91232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-ITCH antibody (250-300) {FITC} (STJ501508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Immunohistochemistry of rat tissue with Anti-SGK1 primary antibody (STJ502978) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 10X.

Anti-SGK1 antibody (40-90) (STJ502978)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£400.50

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Western blot analysis of lysates from HepG2 cells, using TISB (Phospho-Ser92) Antibody. The lane on the right was STJ90549

Anti-Phospho-ZFP36L1-Ser92 antibody (58-107 aa) (STJ90549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot with Anti-ITCH Antibody (STJ501506) of THP cell at 1:500 dilution in buffer.

Anti-ITCH antibody (250-300) (STJ501506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-SGK1 antibody (40-90) {FITC} (STJ502980)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Immunohistochemistry of baboon kidney with Anti-ZFP36L1 primary antibody (STJ503573) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ZFP36L1 antibody (200-250) (STJ503573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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ZFP36L1 Positive Control for STJ503573 peptide (STJ505475)

Applications :

WB

£308.50

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Human Atrophin-1 interacting protein 4/ITCH protein (Recombinant) (NO-Tag) (STJP020387)

Host :

E.coli

£427.50

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SGK1 antibody immunohistochemistry human brain tissue STJA0032892

Anti-SGK1 antibody [6E4-8G5-6D10] (STJA0032892)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Western blot analysis of various lysates using ITCH Rabbit mAb (STJ11102343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ITCH antibody (1-100) [S3MR] (STJ11102343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of L929 cells using SGK1 Rabbit polyclonal antibody (STJ25513) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGK1 antibody (172-431) (STJ25513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-SGK1 antibody (40-90) {Biotin} (STJ502979)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Anti-ZFP36L1 antibody (200-250) {Biotin} (STJ503574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Immunohistochemistry analysis of ITCH in paraffin-embedded mouse brain using ITCH Rabbit polyclonal antibody (STJ11103875) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITCH antibody (1-155) (STJ11103875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Recombinant-ITCH antibody [RM2M15] (STJA0026078)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-ITCH antibody [RM2M17] (STJA0026080)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£300.50 - £457.50

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ITCH/AIP4 antibody western blot A549 HL-60 STJA0035242

Anti-ITCH antibody [R03-4I6] (STJA0035242)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from mouse brain, using ITCH Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ITCH antibody (386-435 aa) (STJ91517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-ITCH antibody (250-300) {Biotin} (STJ501507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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ITCH/AIP4 Positive Control peptide (STJ504326)

Applications :

WB

£308.50

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SGK1 Blocking Peptide for STJ502980 peptide (STJ504916)

Applications :

Immunodepletion/Immunocompetition

£260.50

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SGK1 Positive Control for STJ502979 peptide (STJ505140)

Applications :

WB

£308.50

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Anti-Recombinant-ITCH antibody [RM2M16] (STJA0026079)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human ZFP36L1 protein (Recombinant) (N-His-SUMO) (STJP008023)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human ARRB1 protein (Recombinant) (N-His) (STJP004813)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ARRB1-Ser412 antibody (369-418 aa) (STJ90802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Arrestin-Beta-1 Polyclonal Antibody diluted at 1：500

Anti-ARRB1 antibody (369-418 aa) (STJ91708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot detection of beta Arrestin 1 in PC12, Raw264.7, MCF7 and mouse brain cell lysates using beta Arrestin 1 mouse mAb (1:500 diluted).Predicted band size:50KDa.Observed band size:50KDa.

Anti-ARRB1 antibody [1H4-F5-C11] (STJ99128)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Rat/Human

£149.00 - £360.00

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Human ARRB1 protein (Recombinant) (C-His) (STJP014515)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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Anti-Recombinant-Phospho-ARRB1-Ser412 antibody [RM1X37] (STJA0024714)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Western blot analysis of lysates from Jurkat and HUVEC cells, using ARRB1 Antibody. The lane on the right was blocked STJ91709

Anti-ARRB1 antibody (369-418 aa) (STJ91709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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betaArrestin 1 antibody western blot K562 HeLa STJA0032399

Anti-beta Arrestin 1 antibody [6A1-D7-C9] (STJA0032399)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using Beta-arrestin1 Rabbit monoclonal antibody (STJ112640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARRB1 antibody (1-100) [S0MR] (STJ112640)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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ARRB1 antibody western blot 293T cells STJA0035876

Anti-ARRB1 antibody [R08-5I9] (STJA0035876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 200 Mu g extracts of SH-SY5Y cells using 3 Mu g Beta-arrestin1 antibody (STJ22692). Western blot was performed from the immunoprecipitate using Beta-arrestin1 (STJ22692) at a dilution of 1:1000.

Anti-ARRB1 antibody (169-418) (STJ22692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Phospho-beta Arrestin 1 Ser412 antibody western blot A549 MCF-7 U251 HL-60 STJA0035053

Anti-Phospho-beta Arrestin 1-Ser412 antibody [R05-6F7] (STJA0035053)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human colon using SIRT1 Rabbit polyclonal antibody (STJ113751) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SIRT1 antibody (448-747) (STJ113751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SIRT1 antibody (448-747) (STJ119437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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