St John's Laboratory Ltd

Western blot analysis of various lysates, using Phospho-POLR2A-T4 monoclonal antibody antibody (STJ11105859) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-POLR2A-T4 antibody [S5859RM] (STJ11105859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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STJ70680 (0. 05µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APOE antibody (C-Term) (STJ70680)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Human ApoE (Apolipoprotein E) Antibody Pair (STJAP00176)

Clonality :

Monoclonal/Monoclonal

Host :

Mouse/Mouse

Applications :

ELISA

Reactivity :

Human

£484.50

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Anti-APOE antibody [SAA0799] (STJA0019266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FuncS/IHC/IP

Reactivity :

Human

£198.50 - £1,170.50

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Anti-APOE antibody [1D7] (STJA0021669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using POLR2A antibody (STJ113726) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-POLR2A antibody (1-260) (STJ113726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-POLR2A antibody (N-Term) [SRM14470] (STJ11109200)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ChIP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-Rpb1 CTD-S2/S5-S2/S5 antibody [SRM15121] (STJ11109851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ChIP/CUT&Tag/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (STJ11103724) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. MCF7 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S7 antibody (STJ11103724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HeLa, using Phospho-POLR2A CTD-S2/S5 antibody (STJ11105825) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-Phospho-POLR2A-S2/S5 antibody (STJ11105825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of 293T cells using ApoE Polyclonal Antibody diluted at 1：500

Anti-APOE antibody (37-86 aa) (STJ91638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ApoE antibody [PT0948R] (STJA0039789)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Chromatin immunoprecipitation analysis of extracts of 293T; cells, using Phospho-POLR2A CTD-T4 antibody (STJ11104227) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Phospho-POLR2A-T4 antibody (STJ11104227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-HIPK2 antibody (STJ191972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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SDS-PAGE gel of recombinant human RACK1 protein 3 µg under reducing conditions with Coomassie stain

Human RACK1 protein (Recombinant) (His-Tag) (STJP017498)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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ApoE antibody western blot human serum STJA0035968

Anti-ApoE antibody [R03-3H7] (STJA0035968)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (STJ116378) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour or treated by Doxorubicin (0. 5uM) for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S2 antibody (STJ116378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug POLR2A Rabbit polyclonal antibody (STJ25045 1:100). Western blot was performed from the immunoprecipitate using POLR2A Rabbit polyclonal antibody (STJ25045) at a dilition of 1:1000.

Anti-POLR2A antibody (1-1970) (STJ25045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using POLR2A Rabbit monoclonal antibody (STJ11103279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-POLR2A antibody (1135-1311) [S9MR] (STJ11103279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RACK1 antibody (STJ192227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-RACK1 antibody (Internal Region) (STJ13101131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Human RACK1 protein (Recombinant) (N-His-MBP) (STJP010071)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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GIT1 antibody western blot 293T cells STJA0036802

Anti-GIT1 antibody [R09-6D6] (STJA0036802)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using RACK1 Rabbit pAb (STJ23821) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-RACK1 antibody (30-290) (STJ23821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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RACK1 antibody western blot C6 3T3 HeLa STJA0034787

Anti-RACK1 antibody [R02-1K6] (STJA0034787)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using RACK1 Rabbit monoclonal antibody (STJ11101953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RACK1 antibody (220-310) [S3MR] (STJ11101953)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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STJ72485 (0. 1µg/ml) staining of Human Lymph Node (A) , Mouse Spleen (B) and Rat Spleen (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-GNB2L1 antibody (Internal) (STJ72485)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-HIPK2 antibody (1150-1198) (STJ501343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-HIPK2 antibody (1150-1198) {Biotin} (STJ501344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of various lysates using HIPK2 Rabbit mAb (STJ11102490) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HIPK2 antibody (100-200) [S0MR] (STJ11102490)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] GIT1 Rabbit polyclonal antibody (STJ117632) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GIT1 antibody (460-640) (STJ117632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Apoe Rabbit polyclonal antibody (STJ118772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Apoe antibody (211-311) (STJ118772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-Recombinant-RACK1 antibody [RM2A70] (STJA0025044)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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RACK1 antibody western blot HeLa lysates STJA0036987

Anti-RACK1 antibody [R03-4K4] (STJA0036987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of HepG2 cells using APOE Rabbit polyclonal antibody (STJ29789) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ApoE antibody (41-307) (STJ29789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Recombinant-RACK1 antibody [RM2A71] (STJA0025045)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-APOE antibody [SRM14963] (STJ11109693)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Apolipoprotein E antibody (STJA0040717)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA

Reactivity :

Human

£528.50

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Immunohistochemistry analysis of HMGB1 in paraffin-embedded mouse heart using HMGB1 Rabbit polyclonal antibody (STJ24039) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HMGB1 antibody (69-215) (STJ24039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HMGB1 antibody (150-200) {FITC} (STJ501360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Human HMGB1 protein (Active Recombinant) (C-His) (STJP000784)

Host :

HEK293 cells

£104.50 - £347.50

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Anti-High-mobility group protein 1 antibody (Full-Length) [1F3] (STJA0040783)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ICC/WB

Reactivity :

Bovine/Human/Mouse/Other Mammals/Pig/Rat

£528.50

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HIPK2 antibody western blot HT-1080 STJA0036915

Anti-HIPK2 antibody [R06-8V2] (STJA0036915)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-HIPK2 antibody (1150-1198) {FITC} (STJ501345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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HIPK2 antibody western blot A549 HL-60 U2OS STJA0035221

Anti-HIPK2 antibody [R06-5G2] (STJA0035221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit polyclonal antibody (STJ11102770) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-HMGB1-K29 antibody (STJ11102770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Human HMGB1-EGFP protein (Recombinant) (C-6His) (STJP001916)

Host :

HEK293 cells

£104.50 - £347.50

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