| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GIT1 (460-640) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GIT1 |
| Gene ID: |
28964 |
| Uniprot ID: |
GIT1_HUMAN |
| Immunogen Region: |
460-640 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2). |
| Immunogen Sequence: |
EIHKLQAENLQLRQPPGPVP TPPLPSERAEHTPMAPGGST HRRDRQAFSMYEPGSALKPF GGPPGDELTTRLQPFHSTEL EDDAIYSVHVPAGLYRIRKG VSASAVPFTPSSPLLSCSQE GSRHTSKLSRHGSGADSDYE NTQSGDPLLGLEGKRFLELG KEEDFHPELESLDGDLDPGL P |
| Post Translational Modifications | Phosphorylated by PAK1. Phosphorylation on tyrosine residues may be catalyzed by PTK2/FAK1 and SRC in growing fibroblasts. Phosphorylation at Tyr-383 is induced by activation of Ephrin-B1/EFNB1 and catalyzed by SRC family kinases. It is required for the interaction with NCK2 and for GIT1 recruitment to synapses in hippocampal neurons. |
| Function | GTPase-activating protein for ADP ribosylation factor family members, including ARF1. Multidomain scaffold protein that interacts with numerous proteins and therefore participates in many cellular functions, including receptor internalization, focal adhesion remodeling, and signaling by both G protein-coupled receptors and tyrosine kinase receptors. Through PAK1 activation, positively regulates microtubule nucleation during interphase. Plays a role in the regulation of cytokinesis.for this function, may act in a pathway also involving ENTR1 and PTPN13. May promote cell motility both by regulating focal complex dynamics and by local activation of RAC1. May act as scaffold for MAPK1/3 signal transduction in focal adhesions. Recruits MAPK1/3/ERK1/2 to focal adhesions after EGF stimulation via a Src-dependent pathway, hence stimulating cell migration. Plays a role in brain development and function. Involved in the regulation of spine density and synaptic plasticity that is required for processes involved in learning. Plays an important role in dendritic spine morphogenesis and synapse formation. In hippocampal neurons, recruits guanine nucleotide exchange factors (GEFs), such as ARHGEF7/beta-PIX, to the synaptic membrane. These in turn locally activate RAC1, which is an essential step for spine morphogenesis and synapse formation. May contribute to the organization of presynaptic active zones through oligomerization and formation of a Piccolo/PCLO-based protein network, which includes ARHGEF7/beta-PIX and FAK1. In neurons, through its interaction with liprin-alpha family members, may be required for AMPA receptor (GRIA2/3) proper targeting to the cell membrane. In complex with GABA(A) receptors and ARHGEF7, plays a crucial role in regulating GABA(A) receptor synaptic stability, maintaining GPHN/gephyrin scaffolds and hence GABAergic inhibitory synaptic transmission, by locally coordinating RAC1 and PAK1 downstream effector activity, leading to F-actin stabilization. May also be important for RAC1 downstream signaling pathway through PAK3 and regulation of neuronal inhibitory transmission at presynaptic input. Required for successful bone regeneration during fracture healing. The function in intramembranous ossification may, at least partly, exerted by macrophages in which GIT1 is a key negative regulator of redox homeostasis, IL1B production, and glycolysis, acting through the ERK1/2/NRF2/NFE2L2 axis. May play a role in angiogenesis during fracture healing. In this process, may regulate activation of the canonical NF-kappa-B signal in bone mesenchymal stem cells by enhancing the interaction between NEMO and 'Lys-63'-ubiquitinated RIPK1/RIP1, eventually leading to enhanced production of VEGFA and others angiogenic factors. Essential for VEGF signaling through the activation of phospholipase C-gamma and ERK1/2, hence may control endothelial cell proliferation and angiogenesis. |
| Protein Name | Arf Gtpase-Activating Protein Git1Arf Gap Git1Cool-Associated And Tyrosine-Phosphorylated Protein 1Cat-1Cat1G Protein-Coupled Receptor Kinase-Interactor 1Grk-Interacting Protein 1P95-App1 |
| Database Links | Reactome: R-HSA-3928664Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9013420Reactome: R-HSA-9013423Reactome: R-HSA-9013424Reactome: R-HSA-9619229 |
| Cellular Localisation | CytoplasmSynapsePresynapsePostsynapsePostsynaptic DensityCell JunctionFocal AdhesionCell ProjectionLamellipodiumCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleCycles Between At Least 3 Distinct Intracellular CompartmentsIncluding Focal AdhesionsCytosolic ComplexesContaining At Least Pxn/PaxillinArhgef7 And Pak1And Membrane ProtrusionsDuring Cell MigrationMoves From The Disassembling Adhesions Into The Cytosol And Towards The Leading EdgeIn Adherent CellsLocalizes To AdhesionsRecruitment To Adhesions May Be Mediated By Rac And Active Tyrosine-Phosphorylated PxnMay Be Present In Both Excitatory And Inhibitory SynapsesIn Hippocampal NeuronsRecruitment Of Git1 To Synapses Is Regulated By Ephrinb Activation And Ephrinb Downstream Effector Grb4/Nck2Partially Colocalizes With PcloInteraction With Grin3a Limits Git1 Synaptic LocalizationLocalization To The Centrosome Does Not Depend Upon The Presence Of Gamma-Tubulin |
| Alternative Antibody Names | Anti-Arf Gtpase-Activating Protein Git1 antibodyAnti-Arf Gap Git1 antibodyAnti-Cool-Associated And Tyrosine-Phosphorylated Protein 1 antibodyAnti-Cat-1 antibodyAnti-Cat1 antibodyAnti-G Protein-Coupled Receptor Kinase-Interactor 1 antibodyAnti-Grk-Interacting Protein 1 antibodyAnti-P95-App1 antibodyAnti-GIT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
