St John's Laboratory Ltd

Human TNF alpha (TNF alpha) Antibody Pair (STJAP00661)

Clonality :

Monoclonal/Monoclonal

Host :

Mouse/Mouse

Applications :

ELISA

Reactivity :

Human

£497.50

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Human TNF alpha (TNF alpha) Antibody Pair (STJAP00696)

Clonality :

Monoclonal/Monoclonal

Host :

Rabbit/Rabbit

Applications :

ELISA

Reactivity :

Human

£551.50

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Immunofluorescence analysis of U2OS cells using PSMA4 Rabbit polyclonal antibody (STJ25173). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PSMA4 antibody (1-261) (STJ25173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of THP-1, using TNF-Alpha rabbit polyclonal antibody (STJ25882) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-TNF antibody (134-233) (STJ25882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U-2 OS cells using PSMB1 antibody (STJ26164) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PSMB1 antibody (29-241) (STJ26164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-SMAD2-Thr220 antibody (186-235 aa) (STJ91345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot detection of Smad2 in Hela, A431, Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-SMAD2 antibody [6H5-E3-C11] (STJ99174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£149.00 - £360.00

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Anti-PSMB7 antibody (191-241) (STJA0006321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-PSMA2 antibody [RM1S87] (STJA0024269)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human SMAD2 protein (Recombinant) (N-His) (STJP005472)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-Phospho-SMAD2-Ser465/467 antibody [PT0662R] (STJA0039766)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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SDS-PAGE gel of recombinant human PSMA2 protein 3μg under reducing conditions stained with Coomassie blue

Human PSMA2 protein (Recombinant) (His-Tag) (STJP017357)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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SDS-PAGE gel of recombinant human PSMA6 protein 3 μg under reducing conditions Coomassie blue stain

Human PSMA6 protein (Recombinant) (His-Tag) (STJP017432)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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TNF alpha antibody western blot recombinant human protein STJA0032876

Anti-TNF alpha antibody [6H2-4C6-5B6] (STJA0032876)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PSMB2 antibody (STJ115589). Western blot was performed from the immunoprecipitate using PSMB2 antibody (STJ115589) at a dilution of 1:1000.

Anti-PSMB2 antibody (1-201) (STJ115589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Phospho-Smad2 (S467) Polyclonal Antibody diluted at 1：500

Anti-Phospho-SMAD2-Ser467 antibody (418-467 aa) (STJ90744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD2 antibody (418-467 aa) (STJ95694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TNF-Alpha antibody [V1q] (STJ16100926)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Mouse

£713.50

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Western blot analysis of various lysates, using TNF-Alpha antibody (STJ11104757) at1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TNF antibody (77-233) [S4757RM] (STJ11104757)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-Smad2-T8 antibody [SAA0289] (STJA0019134)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Recombinant-Phospho-SMAD2-S255 antibody [RM2G66] (STJA0025634)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-SMAD2-Ser255 antibody [RM2G67] (STJA0025635)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-Recombinant-SMAD2 antibody [RM2G69] (STJA0025637)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Rat/Hamster

£300.50 - £457.50

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Anti-Phospho-Smad2-Ser250 antibody [PT0058R] (STJA0039594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various lysates using PSMB3 Rabbit pAb (STJ111988) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PSMB3 antibody (1-205) (STJ111988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Human mammary cancer cells using TNF-Alpha antibody (STJ113176) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TNF antibody (77-233) (STJ113176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Mouse TNF alpha (Tumor Necrosis Factor) Sandwich ELISA Kit (STJE0000017)

Applications :

ELISA

Reactivity :

Mouse

£423.50

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STJ72350 (0. 3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PSMB7 antibody (Internal) (STJ72350)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow/Pig

£312.50

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Human PSMa5 (Proteasome Subunit Alpha Type 5) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018097)

Applications :

ELISA

Reactivity :

Human

£440.50 - £614.50

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Anti-Recombinant-Phospho-SMAD2-S250 antibody [RM2G65] (STJA0025633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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SDS-PAGE gel of recombinant human Smad2 protein 3µg under reducing conditions Coomassie blue stain

Human Smad2 protein (Recombinant) (His-Tag) (STJP016453)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PSMB2 antibody (STJ27436). Western blot was performed from the immunoprecipitate using PSMB2 antibody (STJ27436) at a dilution of 1:1000.

Anti-PSMB2 antibody (1-201) (STJ27436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using Smad2 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SMAD2-Ser250 antibody (216-265 aa) (STJ90521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti TNF Goat Polyclonal antibody western blot STJ140123

Anti-TNF antibody (STJ140123)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£135.50 - £440.50

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD2-Ser255 antibody (STJA0009832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TNF-Alpha antibody {PE-Cy7} (STJ11109125)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£215.50 - £628.50

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TNF alpha antibody western blot Recombinant Human TNF alpha STJA0032958

Anti-TNF alpha antibody [9D9-6A9-8B4] (STJA0032958)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Western blot analysis of Smad2/3 (phospho Thr8) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-Smad2/3-Thr8 antibody (1-50 aa) (STJ90522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD2-Ser465 antibody (STJ91000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, Smad2/3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Smad2/3 antibody (1-50 aa) (STJ95697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Pig

£49.00 - £261.00

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Anti-TNF-Alpha antibody [T1] (STJ16100934)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/WB

Reactivity :

Human

£586.50

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Anti-Recombinant-SMAD2 antibody [RM2G64] (STJA0025632)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Anti-TNF-Alpha antibody {PE} (STJ11109124)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£183.50 - £523.50

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Human PSMA2 protein (Recombinant) (N-His) (STJP015715)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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SDS-PAGE gel of recombinant human PSMB1 protein 3µg reducing conditions Coomassie stain

Human PSMB1 protein (Recombinant) (His-Tag) (STJP016424)

Host :

E.coli

Applications :

SDS-PAGE

£210.50 - £1,418.50

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SDS-PAGE gel showing recombinant human PSMB2 protein 3µg under reducing conditions with Coomassie blue stain

Human PSMB2 protein (Recombinant) (His-Tag) (STJP017445)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of extracts of various cell lines, using PSMA5 antibody (STJ28319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PSMA5 antibody (1-241) (STJ28319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human PSMA5 protein (Recombinant) (N-His) (STJP004464)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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