Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts of HepG2 cells, using Complement Complement C3 Rabbit monoclonal antibody (STJ11101289) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-C3 antibody (1200-1300) [S9MR] (STJ11101289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Immunofluorescence analysis of paraffin-embedded Human liver using C3c Rabbit polyclonal antibody (STJ11105260) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-C3 antibody (1364-1663 aa) (STJ11105260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of various lysates using Complement C3 Rabbit pAb (STJ11102332) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-C3 antibody (1364-1663) (STJ11102332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-C3 antibody (1501-1505 aa) (STJ192235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-C3 antibody [PT1038R] (STJA0040072)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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C3 antibody western blot human plasma STJA0036726

Anti-C3 antibody [R01-6Z5] (STJA0036726)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$184.23 - $388.47

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C3 antibody western blot K562 HeLa STJA0034096

Anti-C3 antibody [R04-2D8] (STJA0034096)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$184.23 - $388.47

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C3 antibody western blot human serum lysates STJA0034095

Anti-C3 antibody [R06-2C2] (STJA0034095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

$184.23 - $388.47

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C3 antibody western blot HepG2 lysates STJA0036300

Anti-C3 antibody [R06-2D7] (STJA0036300)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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Anti-C3 antibody [SRM14895] (STJ11109625)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-C3 nanobody [SAA0973] (STJN000071)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$269.79 - $1,820.91

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Anti-C3 nanobody [SAA0981] (STJN000072)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$269.79 - $1,820.91

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Anti-C3 nanobody [SAA1166] (STJN000070)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/SPR

Reactivity :

Human

$269.79 - $1,820.91

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Immunofluorescence analysis of paraffin-embedded mouse liver using Complement C3 Rabbit polyclonal antibody (STJ115248) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-C3a antibody (1364-1663) (STJ115248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-C3aR antibody [HC3aRZ8] (STJ16103157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F

Reactivity :

Human

$617.55

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Confocal imaging of paraffin-embedded Mouse heart tissue using C3AR1 Rabbit mAb (STJ11107367, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-C3AR1 antibody (1-100 aa) [SRM5453] (STJ11107367)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

$174.57 - $765.21

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Western blot analysis of lysates from Mouse heart, using C3AR1 Rabbit polyclonal antibody (STJ11105428) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-C3AR1 antibody (1-100) (STJ11105428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using C3AR1 antibody (STJ28444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-C3AR1 antibody (161-337) (STJ28444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of COS7 cells, using C3AR1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-C3AR1 antibody (175-224 aa) (STJ91933)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-C3AR1 antibody [SAA0776] (STJA0027686)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$435.39

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Anti-C3AR1 antibody [SAA0776] {APC} (STJA0029584)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$540.27

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Anti-C3AR1 antibody [SAA0776] {FITC} (STJA0028618)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$435.39

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Anti-C3AR1 antibody [SAA0776] {PerCP} (STJA0030550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$504.39

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Anti-C3AR1 antibody [SAA0776] {PE} (STJA0031516)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$540.27

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Anti-C3d antibody IHC Human transplanted kidney STJ180489

Anti-C3d antibody (Full length) [ZM369] (STJ180489)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$202.17 - $703.11

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Anti-C3d antibody [SAA0493] (STJA0019336)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

Human

$269.79 - $1,820.91

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Western blot analysis of lysates from Mouse lung, using C5/C5a Rabbit pAb (STJ110403) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-C5 antibody (1567-1676) (STJ110403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-C5 antibody (690-770 aa) (STJ192331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-C5 nanobody [SAA1365] (STJN000073)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

BIF/ELISA

Reactivity :

Human

$269.79 - $1,820.91

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Anti-C5/Complement C5 antibody [SAA0831] (STJA0019635)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human

$269.79 - $1,820.91

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Anti-C5AR1 antibody (1-100 aa) [SRM5313] (STJ11107216)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis ofmouse brainrat spleen lysis using C5AR1 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-C5AR1 antibody (1-50 aa) (STJ97663)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using CD88 Polyclonal Antibody

Anti-C5AR1 antibody (301-350 aa) (STJ92146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-C5AR1 antibody (STJ11107780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using C5AR2 Rabbit polyclonal antibody (STJ112602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-C5AR2 antibody (258-337) (STJ112602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-C5AR2 antibody (270-350 aa) (STJ192637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-C5aR2 antibody [1D9-M12] (STJ16103158)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC

Reactivity :

Human/Non-Human Primates

$617.55

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Western blot analysis of various lysates, using CAB39 antibody (STJ118565) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CAB39 antibody (1-341) (STJ118565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CAB39 antibody (STJ190629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-CAB39 antibody (STJ500336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Mouse

$552.69

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CAB39 antibody western blot mouse kidney rat lung STJA0035136

Anti-CAB39 antibody [R03-4G7] (STJA0035136)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-CAB39 antibody {Biotin} (STJ500337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Mouse

$686.55

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Anti-CAB39 antibody {FITC} (STJ500338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Mouse

$686.55

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Western blot analysis of various lysates using CAB39L Rabbit polyclonal antibody (STJ116273) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAB39L antibody (1-337) (STJ116273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-CAB39L antibody (STJ190630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of CALCA in paraffin-embedded mouse spinal cord using CALCA Rabbit polyclonal antibody (STJ11102780) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CALCA antibody (26-128) (STJ11102780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Human thyroid tissue was stained with anti-Calcitonin (ABT124) antibody.

Anti-CALCA antibody (26-141 aa) [ABT124] (STJ197104)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Human thyroid atypical adenoma tissue was stained with anti-Calcitonin (ABT285) antibody.

Anti-CALCA antibody (26-141 aa) [ABT285] (STJ197105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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