Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various lysates using [KO Validated] Caspase-3 rabbit polyclonal antibody (STJ22900) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CASP3 antibody (55-277) (STJ22900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-CASP3 antibody (6-105) (STJ113073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunocytochemistry analysis of Caspase 3 (green) in 293 using Caspase 3 antibody, and DAPI (blue).

Anti-CASP3 antibody (75-94 aa) [R02-7C2] (STJA0010817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

€330.63

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Anti-CASP3 antibody (N-Term) [M377] (STJA0005295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

€399.38

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Anti-CASP3 antibody [31A1067] (STJA0005296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€399.38

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Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP3 antibody [5E1] (STJ97448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IP/IHC

Reactivity :

Human/Mouse/Rat/Chicken

€60.00 - €315.00

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Anti-CASP3 nanobody [VHH1] (STJN000623)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-CASP3 nanobody [VHH2] (STJN000624)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-CASP3/Caspase-3 nanobody [SAA1223] (STJN000220)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF

Reactivity :

Human

€473.13 - €2,040.63

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Anti-CASP8 antibody (200-300) [S2MR] (STJ11101692)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ113797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP8 antibody (300-400) (STJ113797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ29787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Casp8 antibody (376-479) (STJ29787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CASP8 antibody (C-Term) [M382] (STJA0005298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

€399.38

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57 Yang B, Wang L, Luo X, et al. SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis[J]. International journal of oncology, 2015, 46 (2) : 649-656.

Anti-CASP8 antibody (STJ193171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat/Pig/Chicken

€60.00 - €315.00

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Western blot analysis using Caspase-8 monoclonal antibody against HeLa (1) , Jurkat (2) , THP-1 (3) , NIH/3T3 (4) , Cos7 (5) and PC-12 (6) cell lysate.

Anti-CASP8 antibody [1H11] (STJ97895)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€180.00 - €435.00

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP8 antibody [2G12] (STJ97394)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ110935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP9 antibody (139-416) (STJ110935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from wild type (WT) and Caspase-9 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] Caspase-9 Rabbit polyclonal antibody (STJ11104477) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CASP9 antibody (139-416) (STJ11104477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of various lysates using Caspase-9 Rabbit pAb (STJ22908) at 1:300 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CASP9 antibody (139-416) (STJ22908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ113147) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP9 antibody (250-350) (STJ113147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using Caspase-9 Polyclonal Antibody diluted at 1：500

Anti-CASP9 antibody (91-140 aa) (STJ92027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot detection of Caspase-9 in A549, MCF7, Jurkat, K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.

Anti-CASP9 antibody [1D1-F2-E9] (STJ99219)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Transfected

€180.00 - €435.00

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP9 antibody [3-20] (STJ96979)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/Chicken

€60.00 - €315.00

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Anti-CASP9/Caspase 9 antibody (Lys100-Thr270) [1A127] (STJA0021960)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

€286.88 - €410.63

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Western blot analysis of lysates from Mouse kidney, using Cleaved-Caspase 3 p17 Rabbit pAb (STJ11106903) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Caspase-3 antibody (119-218 aa) (STJ11106903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of lysates from Jurkat cells, using Cleaved Caspase-9 P37 Rabbit pAb (STJ11106883) at 1:900 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Caspase-9 antibody (250-350 aa) (STJ11106883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded rat kidney using CASR antibody (STJ22909) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASR antibody (180-536) (STJ22909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CASR antibody (20-300 aa) (STJ11106237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunohistochemistry with Anti-CASR Antibody (STJ500366) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-CASR antibody (47-69) (STJ500366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-CASR antibody (47-69) {Biotin} (STJ500367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CASR antibody (47-69) {FITC} (STJ500368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Calcium Sensing Receptor Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CASR antibody (854-903 aa) (STJ92031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from rat brain, using CASR Rabbit pAb (STJ114969) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CASR antibody (874-1088) (STJ114969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€140.63 - €655.63

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Anti-CaSR antibody (N-Term) (STJ13100918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€414.38

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Anti-CaSR antibody (STJ13100917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

€414.38

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Anti-CASR nanobody [SAA1046] (STJN000216)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Immunofluorescence analysis of HeLa cells using Caveolin-1 Rabbit polyclonal antibody (STJ22912) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CAV1 antibody (1-100) (STJ22912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various lysates, using Caveolin-1 antibody (STJ28041) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CAV1 antibody (1-178) (STJ28041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded Human lung using Caveolin-1 Rabbit monoclonal antibody (STJ11101544) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CAV1 antibody (1-80) [S4MR] (STJ11101544)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Anti-CAV1 antibody at 1:1000 dilution; 100 µg lysate per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CAV1 antibody (100aa N-Term) (STJ140019)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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Immunofluorescence analysis of Hela cell. 1, Caveolin-1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CAV1 antibody (129-178 aa) (STJ92051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-CAV1 antibody (30-80 aa) (STJ13100076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

€414.38

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Anti-CAV1 antibody (30-80 aa) (STJ13100077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

€414.38

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Anti-CAV1 antibody (Full Length) [M281] (STJA0005312)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€488.13

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Anti-CAV1 antibody (N-Term) (STJA0005313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€399.38

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Anti-CAV1/Caveolin-1 antibody [SAA1788] (STJA0020216)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€426.88 - €1,809.38

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Caveolin-2 antibody (STJ11102182). Western blot was performed from the immunoprecipitate using Caveolin-2 antibody (STJ11102182) at a dilution of 1:1000.

Anti-CAV2 antibody (1-100) [S2MR] (STJ11102182)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Anti-CAV2 at 1:1000 dilution; 50 µg lysate per lane of transfected HEK293 and 100 µg of HaCat cell line; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CAV2 antibody (50aa N-Term) (STJ140020)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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