Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various lysates using CAMKK2 Rabbit pAb (STJ111941) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-CAMKK2 antibody (1-100) (STJ111941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of HuvEc cells using CaMKII Beta Polyclonal Antibody

Anti-CAMKK2 antibody (381-430 aa) (STJ91989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using CAPZB Rabbit pAb (STJ110405) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CAPZB antibody (1-272) (STJ110405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CAPZB antibody (STJA0007368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CARM1 antibody (486-603) (STJ22890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-CARM1 antibody (STJA0010244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot detection of PRMT4/CARM1 in Hela, A431 and K562 cell lysates using PRMT4/CARM1 mouse mAb (1:200-1:500 diluted).Predicted band size:63KDa.Observed band size:63KDa.

Anti-CARM1 antibody [2B9-H11-H10] (STJ99037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis using PRMT4 monoclonal antibody against MCF-7 (1) , HeLa (2) , NIH/3T3 (3) , HL-60 (4) , LNcap (5) , Jurkat (6) , PC-3 (7) , Cos7 (8) , and PC-12 (9) cell lysate.

Anti-CARM1 antibody [3H2] (STJ98337)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Monkey

£144.00 - £348.00

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Immunohistochemistry analysis of CASC5 in paraffin-embedded human colon using CASC5 Rabbit polyclonal antibody (STJ115074) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CASC5 antibody (1-300) (STJ115074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Casein Kinase 2 alpha (CSNK2A1) Rabbit polyclonal antibody (STJ11102907) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Casein Kinase 2 alpha antibody (1-391) (STJ11102907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Casein Kinase 2 beta (CSNK2B) in paraffin-embedded mouse testis using Casein Kinase 2 beta (Casein Kinase 2 beta (CSNK2B) ) Rabbit polyclonal antibody (STJ11102908) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Casein Kinase 2 beta antibody (1-215) (STJ11102908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Jurkat, using Caspase-10 Rabbit polyclonal antibody (STJ22895) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CASP10 antibody (220-521) (STJ22895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using Caspase-10 Rabbit pAb (STJ22894) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CASP10 antibody (230-505) (STJ22894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from Jurkat, K562 cells, using Caspase-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CASP10 antibody (Internal) (STJ96459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Caspase-2 Polyclonal Antibody

Anti-CASP2 antibody (123-172 aa) (STJ92020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CASP2 Polyclonal Antibody (STJ28291) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CASP2 antibody (153-452) (STJ28291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Caspase-2 polyclonal antibody (STJ11102181) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP2 antibody (334-452) [S1MR] (STJ11102181)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of Jurkat cells, using Active Caspase-3 Rabbit monoclonal antibody (STJ112894) at 1:500 dilution. Jurkat cells were treated by Staurosporine (1 Mu M) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP3 antibody (1-100) [S4MR] (STJ112894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using pro Caspase 3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CASP3 antibody (1-20 aa) [R06-1B2] (STJA0010811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£246.50

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Western blot analysis of various cells using Caspase-3 Polyclonal Antibody diluted at 1：1000

Anti-CASP3 antibody (116-165 aa) (STJ92021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Fish

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using Caspase-3 p12 antibody (STJ11101753). HeLa cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (176-277) [S3MR] (STJ11101753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-CASP3 antibody (20-100) [5H10] (STJ97730)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IF-P

Reactivity :

Human/Rat/Mouse

£0.00

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Western blot analysis of lysates from wild type (WT) and active + pro Caspase-3 knockout (KO) 293T cells, using [KO Validated] active + pro Caspase-3 Rabbit monoclonal antibody (STJ11101749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP3 antibody (29-175) [S9MR] (STJ11101749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of various lysates using Caspase-3 Mouse monoclonal antibody (STJ119900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP3 antibody (55-160) [S0MM] (STJ119900)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of U2OS cells using [KO Validated] Caspase-3 Rabbit polyclonal antibody (STJ113869) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (55-277) (STJ113869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of PC-12 cells using Caspase 3 antibody (STJ22899) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-CASP3 antibody (55-277) (STJ22899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] Caspase-3 rabbit polyclonal antibody (STJ22900) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CASP3 antibody (55-277) (STJ22900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-CASP3 antibody (6-105) (STJ113073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of Caspase 3 (green) in 293 using Caspase 3 antibody, and DAPI (blue).

Anti-CASP3 antibody (75-94 aa) [R02-7C2] (STJA0010817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£264.50

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Anti-CASP3 antibody (N-Term) [M377] (STJA0005295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£319.50

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Anti-CASP3 antibody [31A1067] (STJA0005296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£319.50

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Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP3 antibody [5E1] (STJ97448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IP/IHC

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Anti-CASP3 nanobody [VHH1] (STJN000623)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Anti-CASP3 nanobody [VHH2] (STJN000624)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Anti-CASP3/Caspase-3 nanobody [SAA1223] (STJN000220)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF

Reactivity :

Human

£378.50 - £1,632.50

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Anti-CASP8 antibody (200-300) [S2MR] (STJ11101692)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ113797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP8 antibody (300-400) (STJ113797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (STJ29787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Casp8 antibody (376-479) (STJ29787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CASP8 antibody (C-Term) [M382] (STJA0005298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£319.50

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57 Yang B, Wang L, Luo X, et al. SPAG6 silencing inhibits the growth of the malignant myeloid cell lines SKM-1 and K562 via activating p53 and caspase activation-dependent apoptosis[J]. International journal of oncology, 2015, 46 (2) : 649-656.

Anti-CASP8 antibody (STJ193171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat/Pig/Chicken

£48.00 - £252.00

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Western blot analysis using Caspase-8 monoclonal antibody against HeLa (1) , Jurkat (2) , THP-1 (3) , NIH/3T3 (4) , Cos7 (5) and PC-12 (6) cell lysate.

Anti-CASP8 antibody [1H11] (STJ97895)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP8 antibody [2G12] (STJ97394)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ110935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CASP9 antibody (139-416) (STJ110935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and Caspase-9 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] Caspase-9 Rabbit polyclonal antibody (STJ11104477) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CASP9 antibody (139-416) (STJ11104477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Caspase-9 Rabbit pAb (STJ22908) at 1:300 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CASP9 antibody (139-416) (STJ22908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ113147) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP9 antibody (250-350) (STJ113147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Caspase-9 Polyclonal Antibody diluted at 1：500

Anti-CASP9 antibody (91-140 aa) (STJ92027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Caspase-9 in A549, MCF7, Jurkat, K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.

Anti-CASP9 antibody [1D1-F2-E9] (STJ99219)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Transfected

£144.00 - £348.00

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