Reproduction | Research Area | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and ACD knockdown (KD) 293T (KD) cells, using ACD Rabbit polyclonal antibody (STJ114070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ACD antibody (1-220) (STJ114070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-ACD antibody (STJA0008363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-ATM-Lys316 antibody (STJA0005921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Acetyl-H2AX-Lys5 antibody (STJ197313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H2BC1-Lys109 antibody (STJA0005940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H2BC1-Lys117 antibody (STJA0005941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H2BC1-Lys121 antibody (STJA0005942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of A549 cells using Acetyl-Histone H2B (K126) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-H2BC1-Lys126 antibody (78-127 aa) (STJ90142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H2BC1-Lys17 antibody (STJA0005943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Acetyl-H2BC12L-K12 antibody (10-59 aa) (STJ90119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€60.00 - €315.00

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Western blot analysis of 3T3 cells using Acetyl-Histone H2B (K15) Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-H2BC12L-Lys15 antibody (1-50 aa) (STJ90120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-H2BC12L-Lys5 antibody (1-50 aa) (STJ90118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-Acetyl-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12-Lys116 antibody (STJA0005948)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12-Lys37 antibody (STJA0005945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12-Lys5 antibody (STJA0005944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12-Lys57 antibody (STJA0005946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12-Lys80 antibody (STJA0005947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of Acetyl-Histone H4-K5/K8/K12/K16 in paraffin-embedded rat testis using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit polyclonal antibody (STJ11104077) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Acetyl-H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4C16-K5/K8/K12/K16 antibody (STJ11104077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Shen, Tong, et al. "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy. " Journal of hematology & oncology 11. 1 (2018) : 95.

Anti-Acetyl-H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16-Lys16 antibody (STJA0006006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16-Lys21 antibody (STJA0005949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Acetyl-H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16-Lys92 antibody (STJA0005950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl Histone H3 K9 Mouse mAb diluted at 1:200.

Anti-Acetyl-Histone H3.1-K9 antibody [2E7] (STJ97749)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 Rabbit polyclonal antibody (STJ118031) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K12 antibody (STJ118031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K16 Rabbit polyclonal antibody (STJ11103217) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Acetyl-Histone H4-K16 antibody (STJ11103217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 (STJ116277) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. U2OS cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K16 antibody (STJ116277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ117427) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Acetyl-Histone H4-K5 antibody (1-13) (STJ117427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody (STJ11103295) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Acetyl-Histone H4-K5 antibody (STJ11103295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ11101677) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Acetyl-Histone H4-K5 antibody [S7MR] (STJ11101677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€183.13 - €803.13

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Anti-Acetyl-Histone H4-K5/K8/K12/K16 antibody [SRM] (STJ11107155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Chromatin immunoprecipitation was performed with 5 Mu g of cross-linked chromatin from HeLa, using 5 Mu g of Acetyl-Histone H4-K8 Rabbit polyclonal antibody (STJ29400) and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Anti-Acetyl-Histone H4-K8 antibody (STJ29400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 Rabbit polyclonal antibody (STJ11100937) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H4-K91 antibody (STJ11100937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

€161.88 - €803.13

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys12) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys12 antibody (10-59 aa) (STJ90128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys16 antibody (1-50 aa) (STJ90129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys5) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys5 antibody (1-50 aa) (STJ90126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys8) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys8 antibody (1-50 aa) (STJ90127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Anti-Acetyl-Histone-H3-K27 antibody (STJ11106370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Acetyl-Histone-H3-K9 antibody (STJ11106371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various cells using Acrosin Polyclonal Antibody

Anti-ACR antibody (40-120 aa) (STJ90000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-ACR antibody (50-150 aa) (STJ11106527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ADAM2 antibody (109-159 aa) (STJ194448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using ADAM2 antibody (STJ112226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ADAM2 antibody (476-686) (STJ112226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-ADAM20 antibody (STJA0004326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

€175.63 - €519.38

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Anti-ADAM30 antibody (400-480 aa) (STJ190513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of H460 cells, using ATM Antibody (STJ27926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATM antibody (1900-2000) (STJ27926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ATM antibody (1920-2000 aa) (STJ91763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis ofmouse brainK562 cells using Atm Polyclonal Antibody diluted at 1：500

Anti-ATM antibody (1950-1999 aa) (STJ91762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunocytochemistry analysis of ATM (green) in HT-1080 using ATM antibody, and DAPI (blue).

Anti-ATM antibody (1950-2050 aa) [R08-8H3] (STJA0010772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

€330.63

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Western blot analysis of lysates from wild type (WT) and ATM knockout (KO) 293T cells, using [KO Validated] ATM Rabbit polyclonal antibody (STJ11100214) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ATM antibody (2320-2566) (STJ11100214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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