Metabolism Of RNA | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using TXNL4A antibody (STJ112177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TXNL4A antibody (1-142) (STJ112177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-TXNL4A antibody (STJA0008677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-TYW3 antibody (STJA0009411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-TYW5 antibody (STJA0008954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-U1A antibody [R09-2J8] (STJA0036808)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-U1C antibody [R09-6E9] (STJA0034970)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g U2AF1 antibody (STJ115132). Western blot was performed from the immunoprecipitate using U2AF1 antibody (STJ115132) at a dilution of 1:1000.

Anti-U2AF1 antibody (1-100) (STJ115132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-U2AF1 antibody (60-140 aa) (STJ190205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of SW620 cells using 1 Mu g U2AF2/U2AF65 antibody (STJ26004). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (STJ26004) at a dilution of 1:500.

Anti-U2AF2 antibody (192-471) (STJ26004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g U2AF2/U2AF65 antibody (STJ11102100). Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody (STJ11102100) at a dilution of 1:1000.

Anti-U2AF2 antibody (376-475) [S0MR] (STJ11102100)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-U2AF2 antibody (STJA0007037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-U2AF2 antibody [R09-1V1] (STJA0036879)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-U2SURP antibody (STJA0008620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunocytochemistry analysis of Ubiquitin 60S Ribosomal Protein L40 (green) in Hela using Ubiquitin 60S Ribosomal Protein L40 antibody, and DAPI (blue).

Anti-UBA52 antibody (39-52 aa) [R05-5D6] (STJA0012632)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-UBA52 antibody (71-120 aa) (STJ95481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Ub antibody at 2500 dilution; cells stimulated with MG132; lysate at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-UBA52 antibody (STJ140085)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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Anti-UBA52 antibody [PT0081R] (STJA0039231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-UBA52 antibody [SRM13090] (STJ11107820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Confocal imaging of MCF7 cells using K48-linkage Specific Ubiquitin Rabbit monoclonal antibody (STJ11101909, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-UBB antibody (1-100) [S9MR] (STJ11101909)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (STJ11101766) at1:1000 dilution. HeLa, NIH/3T3 and C6 cell were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-228) [S6MR] (STJ11101766)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Immunofluorescence analysis of HeLa cells using Ubiquitin Rabbit polyclonal antibody (STJ11100145) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBB antibody (1-229) (STJ11100145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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Western blot analysis of lysates from C6 cells using Ubiquitin Rabbit polyclonal antibody (STJ26009) at 1:900 dilution. C6 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-229) (STJ26009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HepG2 cells using UBC Rabbit polyclonal antibody (STJ29897) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBC antibody (1-100) (STJ29897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBC antibody (160-210 aa) (STJ194141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-UBC/polyUb/Polyubiquitin-C antibody [SAA0328] (STJA0019255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/IP/WB

Reactivity :

Human

£209.50 - £1,333.50

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Anti-Ubiquitin antibody (STJA0040904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Ubiquitin antibody [1B8-8E7-1H7] (STJA0032907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

£177.50 - £261.50

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Anti-Ubiquitin antibody [7F2-4B1-10D3] (STJA0032914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

£133.50 - £281.50

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Anti-Ubiquitin antibody [R01-7F1] (STJA0034973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Ubiquitin antibody [R02-4I6] (STJA0034974)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Anti-Ubiquitin antibody [SRM13312] (STJ11108042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Ubiquitin K48 antibody [R06-2H7] (STJA0034497)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Ubiquitin K63 antibody [R06-2A6] (STJA0036235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Ubiquitin K63 antibody [R06-2H7] (STJA0035398)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from HepG2 cells, using UBL5 Rabbit polyclonal antibody (STJ11103895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-UBL5 antibody (1-73) (STJ11103895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g UPF1/RENT1 antibody (STJ11102240). Western blot was performed from the immunoprecipitate using UPF1/RENT1 antibody (STJ11102240) at a dilution of 1:1000.

Anti-UPF1 antibody (1-100) [S0MR] (STJ11102240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded mouse heart using UPF1/RENT1 antibody (STJ115502) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-UPF1 antibody (270-370) (STJ115502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using UPF1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-UPF1 antibody (299-348 aa) (STJ95414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g UPF1/RENT1 antibody (STJ26046). Western blot was performed from the immunoprecipitate using UPF1/RENT1 antibody (STJ26046) at a dilution of 1:1000.

Anti-UPF1 antibody (819-1118) (STJ26046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using UPF2 rabbit polyclonal antibody (STJ115373) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-UPF2 antibody (1153-1272) (STJ115373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using UPF2 rabbit polyclonal antibody (STJ29171) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-UPF2 antibody (1153-1272) (STJ29171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-UPF3A antibody (190-240 aa) (STJ194832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of L929 cells using UPF3A Rabbit polyclonal antibody (STJ118338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UPF3A antibody (327-476) (STJ118338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-UPF3B antibody (STJA0007940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of lysates from rat eye, using URM1 Rabbit polyclonal antibody (STJ115759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-URM1 antibody (1-63) (STJ115759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-URM1 antibody (STJA0007800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded rat testis using USP39 Rabbit monoclonal antibody (STJ11102757) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-USP39 antibody (200-300) [S7MR] (STJ11102757)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using USP39 Rabbit pAb (STJ111753) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-USP39 antibody (336-565) (STJ111753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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