Anti-UPF2 antibody (1153-1272) (STJ115373)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UPF2 (1153-1272) antibody for use in WB, IHC-P, IP and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UPF2 |
| Gene ID | 26019 |
| Uniprot ID | RENT2_HUMAN |
| Immunogen Region | 1153-1272 |
| Immunogen Sequence | GPPLGGGEGEAESADTMPFV MLTRKGNKQQFKILNVPMSS QLAANHWNQQQAEQEERMRM KKLTLDINERQEQEDYQEML QSLAQRPAPANTNRERRPRY QHPKGAPNADLIFKTGGRRR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA. |
| Protein Name | Regulator Of Nonsense Transcripts 2Up-Frameshift Suppressor 2 HomologHupf2 |
| Database Links | Reactome: R-HSA-9010553Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-Regulator Of Nonsense Transcripts 2 antibodyAnti-Up-Frameshift Suppressor 2 Homolog antibodyAnti-Hupf2 antibodyAnti-UPF2 antibodyAnti-KIAA1408 antibodyAnti-RENT2 antibody |
Information sourced from Uniprot.org