| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UPF1 (819-1118) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UPF1 |
| Gene ID: |
5976 |
| Uniprot ID: |
RENT1_HUMAN |
| Immunogen Region: |
819-1118 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 819-1118 of human UPF1/RENT1 (NP_002902.2). |
| Immunogen Sequence: |
QEVEIASVDAFQGREKDFII LSCVRANEHQGIGFLNDPRR LNVALTRARYGVIIVGNPKA LSKQPLWNHLLNYYKEQKVL VEGPLNNLRESLMQFSKPRK LVNTINPGARFMTTAMYDAR EAIIPGSVYDRSSQGRPSSM YFQTHDQIGMISAGPSHVAA MNIPIPFNLVMPPMPPPGYF GQANGPAAGRGTPKGKTGRG GRQKNRFGLPGPSQTNLPNS QASQDVASQPFSQGALTQG |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated by SMG1.required for formation of mRNA surveillance complexes. |
| Function | RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation.its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S.the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA harboring long 3'UTR by inducing the NMD machinery. Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA. |
| Protein Name | Regulator Of Nonsense Transcripts 1Atp-Dependent Helicase Rent1Nonsense Mrna Reducing Factor 1Norf1Up-Frameshift Suppressor 1 HomologHupf1 |
| Database Links | Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmP-BodyNucleusPerinuclear RegionHyperphosphorylated Form Is Targeted To The P-BodyWhile Unphosphorylated Protein Is Distributed Throughout The CytoplasmLocalized In The Chromatoid Bodies Of Round Spermatids |
| Alternative Antibody Names | Anti-Regulator Of Nonsense Transcripts 1 antibodyAnti-Atp-Dependent Helicase Rent1 antibodyAnti-Nonsense Mrna Reducing Factor 1 antibodyAnti-Norf1 antibodyAnti-Up-Frameshift Suppressor 1 Homolog antibodyAnti-Hupf1 antibodyAnti-UPF1 antibodyAnti-KIAA0221 antibodyAnti-RENT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
