Metabolism Of RNA | Research Area | St John's Laboratory

Anti-PABPC1 antibody (212-262 aa) (STJ192910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using polyclonal antibodyPC1 antibody (STJ117072) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PABPC1 antibody (350-450) (STJ117072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PABPN1 antibody (STJ27837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PABPN1 antibody (1-100) (STJ27837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PABPN1 rabbit monoclonal antibody (STJ11101447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PABPN1 antibody (1-100) [S7MR] (STJ11101447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PABPN1 antibody (16-28 aa) [R02-8B1] (STJA0011740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£246.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PABPN1 antibody (170-250 aa) (STJ191174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PAIP1 antibody (STJ27838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PAIP1 antibody (1-260) (STJ27838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PAIP1 antibody (100-180 aa) (STJ191176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Pan-PKC antibody [SM5008] (STJA0005008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-PAN2 antibody (STJA0007019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PAPOLG antibody (STJA0009607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of MCF7 cells using PARN antibody (STJ29021). Blue: DAPI for nuclear staining.

Anti-PARN antibody (1-280) (STJ29021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-PARN antibody (STJA0007491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PATL1 antibody (263-313 aa) (STJ193795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat brain using hnRNP E1/PCBP1 Rabbit polyclonal antibody (STJ11104507) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCBP1 antibody (100-200) (STJ11104507)

Clonality :

Polyclonal

Host :

rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using hnRNP E1/hnRNP E1/PCBP1 Rabbit polyclonal antibody (STJ111059) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PCBP1 antibody (100-200) (STJ111059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PCBP1 antibody (30-110 aa) (STJ190222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PCBP1 antibody [SAA1597] (STJA0020025)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of extracts of various cell lines, using hnRNP E2/PCBP2 rabbit polyclonal antibody antibody (STJ24914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PCBP2 antibody (199-215) (STJ24914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug hnRNP E2/PCBP2 antibody (STJ11104187). Western blot was performed from the immunoprecipitate using hnRNP E2/PCBP2 antibody (STJ11104187) at a dilution of 1:1000.

Anti-PCBP2 antibody (220-350) [S4187RM] (STJ11104187)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of PCBP2/hnRNP E2 in C6, 3T3 lysates using PCBP2 antibody.

Anti-PCBP2 antibody (275-288 aa) [R01-2F5] (STJA0011753)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Mouse/Rat

£246.50

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Anti-PCBP2 antibody (30-110 aa) (STJ190223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using PCF11 Rabbit polyclonal antibody (STJ118960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PCF11 antibody (1256-1555) (STJ118960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-PCF11 antibody (490-570 aa) (STJ191199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of PDCD7 in paraffin-embedded rat liver tissue using PDCD7 Rabbit monoclonal antibody (STJ11102768) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-PDCD7 antibody (1-100) [S8MR] (STJ11102768)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PDCD7 antibody (300-380 aa) (STJ190378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using PELP1 Rabbit mAb (STJ11102411) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-PELP1 antibody (1000-1100) [S1MR] (STJ11102411)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using PELP1 antibody (STJ115375) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PELP1 antibody (884-1130 aa) (STJ115375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PELP1 antibody (STJA0008426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PES1 antibody (STJA0007400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HT-29 cells, using RNUXA Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PHAX antibody (141-190 aa) (STJ95061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PHF5A antibody (33-83 aa) (STJ195409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot with Anti-Phospho-AKT1 Antibody (STJ502089) with PAKT peptide.1:250 dilution in buffer. From 10% SDS gel.

Anti-Phospho-AKT1 antibody (430-480) (STJ502089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£528.50

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Anti-Phospho-AKT1 antibody (430-480) {Biotin} (STJ502090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£584.50

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Anti-Phospho-AKT1 antibody (430-480) {FITC} (STJ502091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£584.50

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Anti-Phospho-AKT1 antibody [M114] (STJA0005252)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-AKT1-S129 Rabbit pAb (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-Phospho-AKT1-S212 antibody [SAA0279] (STJA0019075)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£378.50 - £1,632.50

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [6F8] (STJ97726)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from C6 cells using Phospho-AKT1-S473 Rabbit pAb (STJ116369) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-AKT1-S473 antibody (STJ116369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-AKT1-S473 antibody [P8H9] (STJA0019074)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/AKT8 murine leukemia virus

£378.50 - £1,632.50

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Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-AKT1-S473 antibody [SAA0300] (STJA0019165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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