• Anti-NCL antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-NCL antibody (500aa C-Term) (STJ140144)


Current Stock:
Host: Goat
Applications: WB
Reactivity: Human, Rat, Mouse, Monkey, Canine
Short Description: Goat polyclonal antibody anti-Nucleolin (500aa C-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 1 mg/ml
Dilution Range: WB 1:1000-1:5000
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: NCL
Gene ID: 4691
Immunogen Region: 500aa C-Term
Accession Number: ENSG00000115053
Specificity: Detects endogenous levels of NCL by Western blot.
Immunogen: Purified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli.
Post Translational Modifications Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. Symmetrically methylated by PRMT5.
Function Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Protein Name Nucleolin
Protein C23
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Nucleolin antibody
Anti-Protein C23 antibody
Anti-NCL antibody

Information sourced from Uniprot.org

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