Metabolism Of Proteins | Research Area | St John's Laboratory

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PSA (ABT114) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LnCapPredicted band size: 28kDaObserved band size: 35kDa

Anti-KLK3 antibody (100-200 aa) [ABT114] (STJ197185)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Western blot analysis using PSA monoclonal antibody against truncated PSA recombinant protein.

Anti-KLK3 antibody (26-251 aa) [5A11E9/5A11E2] (STJ98341)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Immunoprecipitation analysis of 300 Mu g extracts of LNCaP cells using 3 Mu g KLK3 antibody (STJ24333). Western blot was performed from the immunoprecipitate using KLK3 antibody (STJ24333) at a dilution of 1:2000.

Anti-KLK3 antibody (30-261) (STJ24333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using Prostate-specific Antigen Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-KLK3 antibody (81-130 aa) (STJ95241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-KLK3 antibody [8301] (STJ400166)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£446.50 - £574.50

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Anti-KLK3 antibody [8311] (STJ400167)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£446.50 - £574.50

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Anti-KLK3 antibody [8312] (STJ400168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£446.50 - £574.50

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Anti-KLK3 antibody [8313] (STJ400169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£446.50 - £574.50

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Western blot analysis of extracts of 293T cells, using Kininogen 1 (Kininogen 1 (KNG1) ) Rabbit polyclonal antibody (STJ115855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-KNG1 antibody (148-427) (STJ115855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HepG2 cells using Kininogen 1 (KNG1) Rabbit polyclonal antibody (STJ24339) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-KNG1 antibody (148-427) (STJ24339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using Kininogen 1 (KNG1) (KNG1) Rabbit monoclonal antibody (STJ11101374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-KNG1 antibody (545-644) [S4MR] (STJ11101374)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-KNG1 antibody (STJ197362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using KTN1 antibody (STJ114330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KTN1 antibody (1-100) (STJ114330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human prostate using KTN1 antibody (STJ28152) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-KTN1 antibody (1-100) (STJ28152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-KTN1 antibody (485-535) (STJA0006489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using L3MBTL2 antibody (STJ112369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-L3MBTL2 antibody (486-705) (STJ112369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-L3MBTL2 IP antibody [SAA0208] (STJA0018723)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Lactic acid-Histone H4-K5-Lactic acid K5 antibody [SRM13380] (STJ11108110)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Anti-Lactoferrin antibody (STJ16101307)

Clonality :

Polyclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human

£724.50

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Anti-Lactoferrin antibody [265-1K1] (STJ16100779)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/WB

Reactivity :

Human

£662.50

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Western blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit polyclonal antibody (STJ11100846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LAMB1 antibody (1467-1786) (STJ11100846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Confocal imaging of C6 cells using Laminin beta 1 Rabbit monoclonal antibody (STJ11102059, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-LAMB1 antibody (1687-1786) [S9MR] (STJ11102059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using Laminin Beta-1 monoclonal antibody against truncated Laminin Beta-1-His recombinant protein (1).

Anti-LAMB1 antibody (31-270 aa) [2D9G5] (STJ98214)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-LAMB1 antibody [PT0479R] (STJA0039503)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-LAMB2 antibody (61-110 aa) (STJ93894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using LAMC1 Rabbit mAb (STJ11107365) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-LAMC1 antibody (1030-1275 aa) [SRM5451] (STJ11107365)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Immunohistochemistry analysis of LAMC1 in paraffin-embedded human uterine cancer using LAMC1 Rabbit polyclonal antibody (STJ118460) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-LAMC1 antibody (1030-1608) (STJ118460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of customer's (cat sample) using Laminin Gamma-1 Polyclonal Antibody diluted at 1：1000

Anti-LAMC1 antibody (1451-1500 aa) (STJ93896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Cat

£49.00 - £261.00

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Anti-LAMC1 antibody [M046] (STJA0005458)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB-Nat/ICC

Reactivity :

Human

£365.50

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Anti-LAMC1 antibody [SRM13467] (STJ11108197)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-LARGE1 antibody (STJA0009340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using LARS Rabbit polyclonal antibody (STJ117090) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LARS antibody (1-270) (STJ117090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-LARS1 antibody (STJA0007719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-LARS2 antibody (832-882 aa) (STJ198025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) Mouse Skeletal Muscle, 3) Mouse Liver, 4) Rat Kidney, diluted at 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-LDHD antibody (STJ197354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LEO1 antibody (159-209 aa) (STJ196067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using LEO1 Rabbit pAb (STJ11103634) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-LEO1 antibody (340-570) (STJ11103634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of NIH/3T3 cells using Leptin Rabbit polyclonal antibody (STJ111070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-LEP antibody (1-100) (STJ111070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human-placenta, antibody was diluted at 1:100

Anti-LEP antibody (10-50 aa) (STJ98979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human prostate using Leptin antibody (STJ27640) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-LEP antibody (22-167) (STJ27640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£110.50 - £505.50

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Indirect ELISA assay for anti-Leptin mouse mAb.Antigen coating concentration: 4ug/ml.

Anti-LEP antibody [5C11-G6-B12] (STJ99268)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-LEP/Leptin antibody [SAA1775] (STJA0020203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Anti-Lewis b antibody [2-25LE] (STJ16100268)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB/IF/IHC

Reactivity :

Human/Guinea Pig

£287.50 - £1,324.50

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Immunofluorescence analysis of NIH-3T3 cells using Galectin 1/LGALS1 Rabbit monoclonal antibody (STJ11102141) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-LGALS1 antibody (1-100) [S1MR] (STJ11102141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from wild type (WT) and Galectin 1/Galectin 1/LGALS1 knockout (KO) 293T cells, using [KO Validated] Galectin 1/LGALS1 Rabbit polyclonal antibody (STJ11100013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-LGALS1 antibody (1-135) (STJ11100013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U2OS cells using Galectin 1/LGALS1 Rabbit polyclonal antibody (STJ111097) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-LGALS1 antibody (1-135) (STJ111097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 cells using Galectin-1 Polyclonal Antibody diluted at 1：500

Anti-LGALS1 antibody (10-90 aa) (STJ93201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Deer

£49.00 - £261.00

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Western blot analysis of Galectin 1 in 3T3 lysates using Galectin 1 antibody.

Anti-LGALS1 antibody (97-110 aa) [R04-0C8] (STJA0012383)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Mouse/Rat/Hamster

£264.50

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