Immune System | Research Area | St John's Laboratory

Western blot analysis of lysates from rat lung, using CXCL2 Rabbit polyclonal antibody (STJ114513) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CXCL2 antibody (1-107) (STJ114513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Anti-CXCL2 antibody (58-107) (STJA0010146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CXCL2/GRO-beta/MIP2-alpha antibody [SAA1544] (STJA0019972)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/Neut

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of Recombinant Human IL-8 Protein using IL8 Rabbit polyclonal antibody (STJ114326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng/1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CXCL8 antibody (1-99) (STJ114326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using IL-8 Polyclonal Antibody

Anti-CXCL8 antibody (19-68 aa) (STJ93708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from THP-1 cells using IL8 Rabbit polyclonal antibody (STJ24194) at 1:500 dilution. THP-1 cells were treated by PMA (80 nM) at 37 °C for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CXCL8 antibody (28-99) (STJ24194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, IL-8 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CXCL8 antibody (50-99 aa) (STJ96518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carrcinoma Tissue using IL-8 Mouse mAb diluted at 1:200.

Anti-CXCL8 antibody (C-Term) [13F8] (STJ97717)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Anti-CXCL8 antibody [1501] (STJ400116)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£446.50 - £702.50

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Western blot detection of IL8 in CHO-K1 transfected by IL8-PDGFR fusion protein cell lysate using IL8 mouse mAb (1:500 (A) -1:1000 (B) diluted).

Anti-CXCL8 antibody [6E7-C11-C9] (STJ99047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer, antibody was diluted at 1:100

Anti-CXCR1 antibody (1-50 aa) (STJ99002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Anti-CXCR1 antibody (272-350) (STJ500640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC-P/IP/WB

Reactivity :

Human

£427.50

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Anti-CXCR1 antibody (272-350) {Biotin} (STJ500644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC-P/IP/WB

Reactivity :

Human

£528.50

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Anti-CXCR1 antibody (272-350) {FITC} (STJ500645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC-P/IP/WB

Reactivity :

Human

£528.50

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CXCR1 antibody (STJ118814) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CXCR1 antibody (325-339) (STJ118814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-CXCR1 Antibody (STJ500639) with peptide on at 1:500 dilution in buffer.

Anti-CXCR1 antibody (N-Term) (STJ500639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-CXCR1 antibody (N-Term) {Biotin} (STJ500642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-CXCR1 antibody (N-Term) {FITC} (STJ500643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Western blot analysis of HuvEc cells using IL-8R Beta Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CXCR2 antibody (1-50 aa) (STJ96762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot with Anti-CXCR2 Antibody (STJ500648) with peptide on at 1:500 dilution in buffer.

Anti-CXCR2 antibody (18-39) (STJ500648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/FC/WB

Reactivity :

Human/Mouse/Rat

£466.50

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Anti-CXCR2 antibody (18-39) {Biotin} (STJ500650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/FC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CXCR2 antibody (18-39) {FITC} (STJ500651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/FC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of Jurkat cells using CXCR2 Rabbit polyclonal antibody (STJ11100902) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CXCR2 antibody (260-359) (STJ11100902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CXCR2 antibody (N-Term) (STJ13100946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various lysates, using CYB5R3 antibody (STJ11104098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CYB5R3 antibody (1-100) [S4098RM] (STJ11104098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CYB5R3 antibody (STJ29673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CYB5R3 antibody (125-334) (STJ29673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using CYB5R3 Polyclonal Antibody

Anti-CYB5R3 antibody (137-186 aa) (STJ92531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CYB5R3 antibody (27-301) (STJ11105448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CYB5R3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CYB5R3 antibody (51-64 aa) [R01-9F3] (STJA0011188)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

£264.50

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Western blot analysis of RAW264. 7, using CYBA antibody (STJ116414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CYBA antibody (126-195) (STJ116414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CYBA antibody (80-160 aa) (STJ191837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (STJ11101774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CYBB antibody (100-200) [S4MR] (STJ11101774)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of RAW 264. 7, using NOX2/gp91phox Rabbit polyclonal antibody (STJ114304) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CYBB antibody (101-200) (STJ114304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of K562 cells using gp91-phox Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-CYBB antibody (111-160 aa) (STJ96748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using NOX2/gp91phox Rabbit pAb (STJ23306) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CYBB antibody (385-570) (STJ23306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CYBB/NOX2 antibody [SAA0847] (STJA0027712)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-CYBB/NOX2 antibody [SAA0847] {APC} (STJA0029000)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£310.50 - £472.50

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Anti-CYBB/NOX2 antibody [SAA0847] {FITC} (STJA0028034)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-CYBB/NOX2 antibody [SAA0847] {PerCP} (STJA0029966)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£292.50 - £440.50

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Anti-CYBB/NOX2 antibody [SAA0847] {PE} (STJA0030932)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£310.50 - £472.50

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Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (STJ11102750) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Cyclin D1 antibody (200-295) (STJ11102750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Cyclophilin A antibody (55-154 aa) [SRM] (STJ11107170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CYFIP1 antibody (STJ112329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CYFIP1 antibody (1-270) (STJ112329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CYFIP1 antibody (980-1060 aa) (STJ191750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HEPG2 Hela cells using CYFIP2 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-CYFIP2 antibody (1171-1220 aa) (STJ98613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Mouse brain tissue using CYLD Rabbit polyclonal antibody (STJ11105647) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CYLD antibody (1-127) (STJ11105647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of CYLD in paraffin-embedded mouse spleen using CYLD Rabbit polyclonal antibody (STJ23310) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CYLD antibody (654-953) (STJ23310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CYLD antibody (STJ193051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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