Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using ApoB Rabbit monoclonal antibody (STJ11102012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-APOB antibody (50-150) [S2MR] (STJ11102012)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Anti-APOB antibody (STJA0006288)

Clonality :

Monoclonal

Host :

Camel

Applications :

FC/ELISA

Reactivity :

Human/Mouse

£141.00 - £144.00

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Anti-APOB antibody (STJA0010126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-APOB antibody [2101] (STJ400014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£455.50 - £870.50

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Western blot analysis using ApoB monoclonal antibody against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 515.6 kDa)

Anti-APOB antibody [6G6] (STJ97838)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of HeLa cells using APP antibody (STJ112892) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APP antibody (1-100) (STJ112892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of U-87MG cells, using APP antibody (STJ118700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-APP antibody (1-100) (STJ118700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from SKOV3 cells, using APP Rabbit polyclonal antibody (STJ22657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APP antibody (1-100) (STJ22657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human esophagus using APP antibody (STJ22656) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-APP antibody (200-300) (STJ22656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-amyloid-Alpha-beta (PTR2007) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SH-SY5YPredicted band size: 86kDaObserved band size: 95kDa

Anti-APP antibody (200-300) [PTR2007] (STJA0006225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-APP antibody (221-270 aa) (STJ98585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-APP antibody (350-450 aa) (STJ13100611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-APP antibody (400-450 aa) (STJ13100557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-APP antibody (400-450 aa) (STJ13100558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of 3T3 Hela cells using Amyloid-Beta Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-APP antibody (631-680 aa) (STJ98604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunocytochemistry analysis of Amyloid Precursor Protein (green) in Hela using Amyloid Precursor Protein antibody, and DAPI (blue).

Anti-APP antibody (66-82 aa) [R09-3F7] (STJA0010881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Western blot analysis of Amyloid Precursor Protein in Hela lysates using Amyloid Precursor Protein antibody.

Anti-APP antibody (671-687 aa) [R01-9E9] (STJA0010885)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£246.50

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Immunofluorescence analysis of SH-SY5Y cells using APP Rabbit polyclonal antibody (STJ11103984) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APP antibody (671-770) (STJ11103984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g [KO Validated] APP Rabbit monoclonal antibody (STJ11101455). Western blot was performed from the immunoprecipitate using [KO Validated] APP Rabbit monoclonal antibody (STJ11101455) at a dilution of 1:1000.

Anti-APP antibody (671-770) [S5MR] (STJ11101455)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-APP antibody (711-760 aa) (STJ91591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-APP antibody (731-737 aa) [R05-3E3] (STJA0010775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Immunohistochemical analysis of paraffin-embedded human brain tissue. 1, Polyclonal Antibody was diluted at 1:200 (4°C overnight). 2, EDTA pH 9.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min) Negtive control was used by secondary antibody only.)

Anti-APP antibody (STJ193176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Hamster

£48.00 - £252.00

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Western blot analysis using APP monoclonal antibody against truncated APP recombinant protein.

Anti-APP antibody [1B11F3] (STJ97844)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of NIH/3T3 cells using APRT Rabbit polyclonal antibody (STJ115881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APRT antibody (1-180) (STJ115881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293T, using CALCA Rabbit polyclonal antibody (STJ27409) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-APRT antibody (1-180) (STJ27409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-APRT antibody (STJA0008078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-AREL1 antibody (354-404 aa) (STJ198007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and ARF1 knockout (KO) HeLa cells, using [KO Validated] ARF1 Rabbit polyclonal antibody (STJ11100853) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARF1 antibody (1-181) (STJ11100853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using ARF1 antibody (STJ11104363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ARF1 antibody (1-181) (STJ11104363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ARF1 antibody (STJ22666) at 1:500 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-ARF1 antibody (100-181) (STJ22666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ARF1 rabbit monoclonal antibody (STJ11102451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ARF1 antibody (70-150) [S1MR] (STJ11102451)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-ARF1 antibody (STJA0006869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Arginase 1 (ARG1) Rabbit polyclonal antibody (STJ26182) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARG1 antibody (1-322) (STJ26182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates of HepG2 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Arginase-1 antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 35 kDa

Anti-ARG1 antibody (200-322 aa) [ABT-Arg1] (STJ197017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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HepG2 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Arginase-1 (ABT262) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 35kDaObserved band size: 37kDa

Anti-ARG1 antibody (200-322 aa) [ABT262] (STJ197099)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of 293 cells using Arginase I Polyclonal Antibody

Anti-ARG1 antibody (61-110 aa) (STJ91676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human hepatic adenoma stained with anti-arginase-1 polyclonal antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and nuclear staining of hepatocytes.

Anti-Arginase-1 antibody [ZR368] (STJ180486)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£194.50 - £768.50

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Western blot analysis of extracts of various cell lines, using ARHGAP45 antibody (STJ112483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ARHGAP45 antibody (1-160) (STJ112483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Anti-ARHGAP45 antibody (STJA0009676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from LOVO cells, using RHG9 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARHGAP9 antibody (220-269 aa) (STJ91685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ARIH1 Rabbit polyclonal antibody (STJ11102851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARIH1 antibody (400 aa) (STJ11102851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ARIH2 antibody (STJA0004349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-ARIH2 antibody (STJA0004350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-ARIH2 antibody (STJA0004351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-ARIH2 antibody (STJA0007577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ARL8A antibody (STJA0007296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ARMC8 antibody (STJA0004566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-ARMC8 antibody (STJA0004567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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