• Confocal imaging of U-2 OS cells using ALOX5 Rabbit monoclonal antibody (STJ11101834, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of ALOX5 in paraffin-embedded mouse spleen using ALOX5 Rabbit monoclonal antibody (STJ11101834) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ALOX5 in paraffin-embedded human appendix using ALOX5 Rabbit monoclonal antibody (STJ11101834) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat lung, using ALOX5 Rabbit monoclonal antibody (STJ11101834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of lysates from Raji cells, using ALOX5 Rabbit monoclonal antibody (STJ11101834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALOX5 antibody (100-200) [S4MR] (STJ11101834)

SKU:
STJ11101834

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ALOX5 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALOX5
Gene ID: 240
Uniprot ID: LOX5_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ALOX5 (P09917).
Immunogen Sequence: CYRWITGDVEVVLRDGRAKL ARDDQIHILKQHRRKELETR QKQYRWMEWNPGFPLSIDAK CHKDLPRDIQFDSEKGVDFV LNYSKAMENLFINRFMHMFQ S
Post Translational Modifications Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-524 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. Phosphorylation at Ser-524 is stimulated by 8-bromo-3',5'-cyclic AMP or prostaglandin E2.
Function Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells.
Protein Name Polyunsaturated Fatty Acid 5-Lipoxygenase
Arachidonate 5-Lipoxygenase
5-Lo
5-Lipoxygenase
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142691
Reactome: R-HSA-2142700
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Reactome: R-HSA-9012546
Reactome: R-HSA-9018676
Reactome: R-HSA-9018682
Reactome: R-HSA-9018896
Reactome: R-HSA-9020265
Reactome: R-HSA-9023661
Reactome: R-HSA-9026286
Reactome: R-HSA-9026290
Reactome: R-HSA-9026403
Reactome: R-HSA-9027604
Cellular Localisation Cytoplasm
Nucleus Matrix
Nucleus Membrane
Peripheral Membrane Protein
Perinuclear Region
Cytosol
Nucleus Envelope
Nucleus Intermembrane Space
Shuttles Between Cytoplasm And Nucleus
Found Exclusively In The Nucleus
When Phosphorylated On Ser-272
Calcium Binding Promotes Translocation From The Cytosol And The Nuclear Matrix To The Nuclear Envelope And Membrane Association
Alternative Antibody Names Anti-Polyunsaturated Fatty Acid 5-Lipoxygenase antibody
Anti-Arachidonate 5-Lipoxygenase antibody
Anti-5-Lo antibody
Anti-5-Lipoxygenase antibody
Anti-ALOX5 antibody
Anti-LOG5 antibody

Information sourced from Uniprot.org

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