Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of HDAC3 in (1) HeLa lysates; (2) NIH/3T3 lysates using HDAC3 antibody.

Anti-HDAC3 antibody (400-428 aa) [R07-8E9] (STJA0014653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis using HDAC3 monoclonal antibody against THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3, HeLa cell lysate.

Anti-HDAC3 antibody (STJ98496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Rabbit

£144.00 - £348.00

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Western blot detection of HDAC3 in THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3 and Hela cell lysates using HDAC3 mouse mAb (1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa.

Anti-HDAC3 antibody [3G3-H6-H10] (STJ99157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of various lysates, using HDAC4 antibody (STJ11101423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HDAC4 antibody (1-100) [S3MR] (STJ11101423)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of HDAC4 in paraffin-embedded mouse brain using HDAC4 Rabbit polyclonal antibody (STJ113153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC4 antibody (451-650) (STJ113153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using HDAC4 Rabbit polyclonal antibody (STJ29788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC4 antibody (530-630) (STJ29788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using HDAC4 Polyclonal Antibody diluted at 1：2000

Anti-HDAC4 antibody (598-647 aa) (STJ93480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using HDAC4 monoclonal antibody against Jurkat, K562 cell lysate.

Anti-HDAC4 antibody (N-Term) (STJ98497)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot detection of HDAC4 in Jurkat, Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa.

Anti-HDAC4 antibody [4A3-H7-C6] (STJ99035)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using NY-CO-9 Polyclonal Antibody

Anti-HDAC5 antibody (1073-1122) (STJ94582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using HDAC5 antibody (STJ29269). Blue: DAPI for nuclear staining.

Anti-HDAC5 antibody (350-450) (STJ29269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using HDAC5 Polyclonal Antibody

Anti-HDAC5 antibody (464-513 aa) (STJ93481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A-549 cells using HDAC5 Rabbit polyclonal antibody (STJ113548).

Anti-HDAC5 antibody (500-600) (STJ113548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC6 antibody (STJ11103524). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ11103524) at a dilution of 1:1000.

Anti-HDAC6 antibody (1-100) [S4MR] (STJ11103524)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using HDAC6 antibody (STJ23932) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC6 antibody (1-200) (STJ23932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HDAC6 (ABT-HDAC6) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 131kDaObserved band size: 130, 180kDa

Anti-HDAC6 antibody (1100-1215 aa) [ABT-HDAC6] (STJ196790)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse

£174.00 - £252.00

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Western blot analysis of various cells using Histone deacetylase 6 Polyclonal Antibody diluted at 1：2000

Anti-HDAC6 antibody (1166-1215 aa) (STJ93512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using HDAC6 antibody (STJ113792).

Anti-HDAC6 antibody (160-470) (STJ113792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts from K-562 cells, using HDAC6 Rabbit monoclonal antibody (STJ11104740) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-HDAC6 antibody (600-700) [S4740RM] (STJ11104740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of Hela cell. 1, HDAC6 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HDAC6 antibody (7-56 aa) (STJ93483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g HDAC6 antibody (STJ113047). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ113047) at a dilution of 1:1000.

Anti-HDAC6 antibody (836-1104) (STJ113047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HDAC6 Rabbit polyclonal antibody (STJ113748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HDAC6 antibody (836-1104) (STJ113748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HDAC6 antibody [SM4991] (STJA0004991)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunohistochemistry analysis of HDAC7 in paraffin-embedded mouse spinal cord using HDAC7 Rabbit polyclonal antibody (STJ11100893) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC7 antibody (1-100) (STJ11100893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using HDAC7 Polyclonal Antibody diluted at 1：1000

Anti-HDAC7 antibody (121-170 aa) (STJ93484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HDAC7 Rabbit monoclonal antibody (STJ11101421) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HDAC7 antibody (24-198) [S1MR] (STJ11101421)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using HDAC7 antibody (STJ29424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HDAC7 antibody (400-500) (STJ29424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Histone deacetylase 7a Polyclonal Antibody

Anti-HDAC7 antibody (901-950 aa) (STJ93513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HDAC8 rabbit monoclonal antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HDAC8 antibody (1-100) [S7MR] (STJ11102387)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using HDAC8 antibody (STJ28392) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC8 antibody (1-377) (STJ28392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using HDAC8 Polyclonal Antibody diluted at 1：1000

Anti-HDAC8 antibody (5-54 aa) (STJ93485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemistry analysis of HDAC9 in paraffin-embedded rat lung using HDAC9 Rabbit polyclonal antibody (STJ29858) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HDAC9 antibody (1-100) (STJ29858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of K-562 cells using HDAC9 Rabbit monoclonal antibody (STJ11101796, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HDAC9 antibody (1-100) [S6MR] (STJ11101796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Anti-HENMT1 antibody (STJA0004480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Anti-HENMT1 antibody (STJA0004481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Human breast infiltrating ductal carcinoma stained with anti-Her-2/neu antibody using peroxidase-conjugate and DAB chromogen. Note the strong membrane staining (3+) of carcinoma cells.

Anti-Her-2/neu antibody [ZR218] (STJ180517)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£200.50 - £842.50

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Western blot analysis of extracts of rat testis, using HES1 antibody (STJ113304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HES1 antibody (151-280) (STJ113304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat/Mouse

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Rat testis cells using 3 Mu g HES1 antibody (STJ11101262). Western blot was performed from the immunoprecipitate using HES1 antibody (STJ11101262) at a dilution of 1:500.

Anti-HES1 antibody (181-280) [S2MR] (STJ11101262)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates, using HES1 antibody (STJ113317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HES1 antibody (90-165) (STJ113317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HES1 antibody (STJ192322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A431 cells using HEY1 antibody (STJ118550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HEY1 antibody (114-129) (STJ118550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HEY1 antibody (STJ193066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using HEY2 antibody (STJ117337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HEY2 antibody (17-32) (STJ117337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, using HEY2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-HEY2 antibody (21-70 aa) (STJ93595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-HIPK1 antibody (290-370 aa) (STJ190111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HIPK1 antibody (700-750) (STJ501340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-HIPK1 antibody (700-750) {Biotin} (STJ501341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIPK1 antibody (700-750) {FITC} (STJ501342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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