Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (STJ11101574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CCNA2 antibody (11-210) [S4MR] (STJ11101574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunofluorescence analysis of U2OS cells using Cyclin A2 antibody (STJ29947) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CCNA2 antibody (350-432) (STJ29947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CCNA2 antibody (STJA0006733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat cells, treated with UV 15', using Cyclin B1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCNB1 antibody (121-170 aa) (STJ92535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ11101575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CCNB1 antibody (3-177) [S5MR] (STJ11101575)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ118478) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCNB1 antibody (334-433) (STJ118478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ22943) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCNB1 antibody (334-433) (STJ22943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HepG2 cells, using Cyclin B1 Rabbit polyclonal antibody (STJ11103802). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CCNB1 antibody (350-433) (STJ11103802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunofluorescence analysis of PC-12 cells using [KO Validated] Cyclin B1 Rabbit polyclonal antibody (STJ119184) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CCNB1 antibody (350-433) (STJ119184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemical analysis of paraffin-embedded Human lung Tissue using Cyclin B1 Mouse mAb diluted at 1:200.

Anti-CCNB1 antibody (60-140 aa) [1A5] (STJ97756)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Cyclin B1 Mouse mAb diluted at 1:200.

Anti-CCNB1 antibody (60-140 aa) [5C1] (STJ97767)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Cyclin B1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CCNB1 antibody (91-140 aa) (STJ92534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis using Cyclin B1 monoclonal antibody against HeLa (1) , Jurkat (2) , K562 (3) and PC-12 (4) cell lysate.

Anti-CCNB1 antibody [1B10] (STJ97976)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis using Cyclin B1 monoclonal antibody against HeLa (1) and PC-12 (2) cell lysate.

Anti-CCNB1 antibody [5G6] (STJ97977)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF/FC/ELISA

Reactivity :

Human/Rat

€180.00 - €435.00

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Western blot analysis of extracts of various cell lines, using Cyclin C antibody (STJ115570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CCNC antibody (1-283) (STJ115570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Cyclin C antibody (STJ28628) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCNC antibody (1-283) (STJ28628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from LOVO cells, using Cyclin C Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCNC antibody (234-283 aa) (STJ92536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-CCNC antibody (STJ99629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry of rat kidney with Anti-CCND1 primary antibody (STJ500672) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CCND1 antibody (1-50) (STJ500672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-CCND1 antibody (1-50) {Biotin} (STJ500678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CCND1 antibody (1-50) {FITC} (STJ500679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CCND1 antibody (10-60) (STJ500673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-CCND1 antibody (10-60) {Biotin} (STJ500680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CCND1 antibody (10-60) {FITC} (STJ500681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Western blot analysis of various lysates, using Cyclin D1 Rabbit monoclonal antibody (STJ11104709) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCND1 antibody (150-250) [S4709RM] (STJ11104709)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Anti-CCND1 Antibody (STJ500674) on rat oesophagus at 1:500 dilution in buffer.

Anti-CCND1 antibody (170-220) (STJ500674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-CCND1 antibody (170-220) {Biotin} (STJ500682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CCND1 antibody (170-220) {FITC} (STJ500683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit monoclonal antibody (STJ11101576, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-CCND1 antibody (196-295) [S6MR] (STJ11101576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g Cyclin D1 antibody (STJ11101272). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (STJ11101272) at a dilution of 1:1000.

Anti-CCND1 antibody (200-295) (STJ11101272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using Cyclin D1 Rabbit polyclonal antibody (STJ29894) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CCND1 antibody (200-295) (STJ29894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry of rat tissue with Anti-CCND1 primary antibody (STJ500675) in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 10X.

Anti-CCND1 antibody (213-232) (STJ500675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human

€534.38

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Anti-CCND1 antibody (213-232) {Biotin} (STJ500684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Rat

€660.63

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Anti-CCND1 antibody (213-232) {FITC} (STJ500685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Rat

€660.63

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Immunofluorescence analysis of Hela cell. 1, Cyclin D1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CCND1 antibody (246-295 aa) (STJ92538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig

€60.00 - €315.00

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Anti-CCND1 antibody (268-295) (STJ500676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€534.38

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Anti-CCND1 antibody (268-295) {Biotin} (STJ500686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€660.63

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Anti-CCND1 antibody (268-295) {FITC} (STJ500687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€660.63

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Anti-CCND1 antibody (280-295) (STJ500677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€534.38

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Anti-CCND1 antibody (280-295) {Biotin} (STJ500688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€660.63

Choose Options

Anti-CCND1 antibody (280-295) {FITC} (STJ500689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€660.63

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Western blot analysis using Cyclin D1 monoclonal antibody against CCND1-hIgGFc transfected HEK293 cell lysate.

Anti-CCND1 antibody [3D8] (STJ97978)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunofluorescence analysis of U2OS cells using Cyclin D2 antibody (STJ115249). Blue: DAPI for nuclear staining.

Anti-CCND2 antibody (1-289) (STJ115249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using Cyclin D2 antibody (STJ26178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CCND2 antibody (1-289) (STJ26178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from 293 cells, using Cyclin D2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CCND2 antibody (240-289 aa) (STJ92539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot detection of Cyclin D2 in CHO-K1 cell lysate (A) and CHO-K1 transfected by Cyclin D2-fragment EGFP fusion protein (B) cell lysate using Cyclin D2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-CCND2 antibody [6E11-G6-F5] (STJ99098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis of extracts of rat liver, using Cyclin D3 antibody (STJ22951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CCND3 antibody (1-292) (STJ22951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€140.63 - €655.63

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Western blot analysis of lysates from wild type (WT) and Cyclin D3 knockout (KO) HeLa cells, using [KO Validated] Cyclin D3 Rabbit polyclonal antibody (STJ22949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CCND3 antibody (201-292) (STJ22949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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