• Western blot analysis of Rat lung, using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of MG-63 cells using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-BGLAP antibody (1-100) (STJ116843)

SKU:
STJ116843

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BGLAP (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BGLAP
Gene ID: 632
Uniprot ID: OSTCN_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Osteocalcin (BGLAP) (NP_954642.1).
Immunogen Sequence: MRALTLLALLALAALCIAGQ AGAKPSGAESSKGAAFVSKQ EGSEVVKRPRRYLYQWLGAP VPYPDPLEPRREVCELNPDC DELADHIGFQEAYRRFYGPV
Post Translational Modifications Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation by GGCX. These residues are essential for the binding of calcium . Decarboxylation promotes the hormone activity.
Function Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation. Functions to limit bone formation without impairing bone resorption or mineralization. It binds strongly to apatite and calcium. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.
Protein Name Osteocalcin
Bone Gla Protein
Bgp
Gamma-Carboxyglutamic Acid-Containing Protein
Database Links Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Reactome: R-HSA-8940973
Cellular Localisation Secreted
Alternative Antibody Names Anti-Osteocalcin antibody
Anti-Bone Gla Protein antibody
Anti-Bgp antibody
Anti-Gamma-Carboxyglutamic Acid-Containing Protein antibody
Anti-BGLAP antibody

Information sourced from Uniprot.org

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