• Western blot analysis of lysates from wild type (WT) and Bid Rabbit monoclonal antibody knockdown (KD) 293T (KD) cells, using Bid Rabbit monoclonal antibody (STJ11105057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from THP-1 cells, using Bid Rabbit monoclonal antibody (STJ11105057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BID antibody (1-195) [S5057RM] (STJ11105057)

SKU:
STJ11105057

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Bh3-Interacting Domain Death Agonist (1-195) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5057RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BID
Gene ID: 637
Uniprot ID: BID_HUMAN
Immunogen Region: 1-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human Bid (NP_001187.1).
Immunogen Sequence: MDCEVNNGSSLRDECITNLL VFGFLQSCSDNSFRRELDAL GHELPVLAPQWEGYDELQTD GNRSSHSRLGRIEADSESQE DIIRNIARHLAQVGDSMDRS IPPGLVNGLALQLRNTSRSE EDRNRDLATALEQLLQAYPR DMEKEKTMLVLALLLAKKVA SHTPSLLRDVFHTTVNFINQ NLRTYVRSLARNGMD
Tissue Specificity Isoform 2: Expressed in spleen, pancreas and placenta (at protein level). Isoform 3: Expressed in lung, pancreas and spleen (at protein level). Isoform 4: Expressed in lung and pancreas (at protein level).
Post Translational Modifications BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products. Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis. BH3-interacting domain death agonist p15: Ubiquitinated by ITCH.ubiquitination results in proteasome-dependent degradation.
Function Induces caspases and apoptosis. Counters the protective effect of BCL2. BH3-interacting domain death agonist p15: Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1: Induces ICE-like proteases and apoptosis. Isoform 2: Induces ICE-like proteases and apoptosis. Isoform 3: Does not induce apoptosis. Isoform 4: Induces ICE-like proteases and apoptosis.
Protein Name Bh3-Interacting Domain Death Agonist
P22 Bid
Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15
P15 Bid - Bh3-Interacting Domain Death Agonist P13
P13 Bid - Bh3-Interacting Domain Death Agonist P11
P11 Bid
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-111452
Reactome: R-HSA-111453
Reactome: R-HSA-114294
Reactome: R-HSA-6803204
Reactome: R-HSA-75108
Cellular Localisation Cytoplasm
Mitochondrion Membrane
Mitochondrion Outer Membrane
When Uncleaved
It Is Predominantly Cytoplasmic
Bh3-Interacting Domain Death Agonist P15: Mitochondrion Membrane
Translocates To Mitochondria As An Integral Membrane Protein
Bh3-Interacting Domain Death Agonist P13: Mitochondrion Membrane
Associated With The Mitochondrial Membrane
Isoform 1: Cytoplasm
Isoform 3: Cytoplasm
Isoform 2: Mitochondrion Membrane
A Significant Proportion Of Isoform 2 Localizes To Mitochondria
It May Be Cleaved Constitutively
Alternative Antibody Names Anti-Bh3-Interacting Domain Death Agonist antibody
Anti-P22 Bid antibody
Anti-Bid Cleaved Into - Bh3-Interacting Domain Death Agonist P15 antibody
Anti-P15 Bid - Bh3-Interacting Domain Death Agonist P13 antibody
Anti-P13 Bid - Bh3-Interacting Domain Death Agonist P11 antibody
Anti-P11 Bid antibody
Anti-BID antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance