Disease | Research Area | St John's Laboratory

Western blot analysis of Hela cells using GRB2 Polyclonal Antibody diluted at 1：2000

Anti-GRB2 antibody (141-190 aa) (STJ93421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot detection of GRB2 in K562, Raji, Jurkat, Ramos, C6, 3T3 and Hela cell lysates using GRB2 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.

Anti-GRB2 antibody [5G3-E7-F11] (STJ99118)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$194.40 - $469.80

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Anti-GRB2 antibody [PT1228R] (STJA0040137)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of mouse liver, using GRPEL1 rabbit polyclonal antibody (STJ27014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GRPEL1 antibody (1-217) (STJ27014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GRPEL1 antibody (159-209 aa) (STJ194944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of L929 cells using GRSF1 Rabbit polyclonal antibody (STJ11100184) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GRSF1 antibody (100-200) (STJ11100184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GRSF1 antibody (302-352 aa) (STJ195011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunocytochemistry analysis of GRSF1 (green) in CEM using GRSF1 antibody, and DAPI (blue).

Anti-GRSF1 antibody (376-389 aa) [R06-2B5] (STJA0012397)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$357.08

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Anti-GRSF1 antibody [SRM13093] (STJ11107823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-GSDMD antibody (1-100) (STJA0010253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GSDMD antibody (STJ11100237). Western blot was performed from the immunoprecipitate using GSDMD antibody (STJ11100237) at a dilution of 1:500.

Anti-GSDMD antibody (346-484) (STJ11100237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using GSDMD Rabbit polyclonal antibody (STJ119438) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSDMD antibody (346-484) (STJ119438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Anti-GSDMD antibody (AA range: 1-100) [PTR1398] (STJA0038893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11106595) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11106595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of GSDMD in Jurkat lysates using GSDMD antibody.

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$357.08

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Anti-GSDMD antibody [SAA1652] (STJA0020080)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$461.03 - $1,954.13

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Anti-GSDME antibody (1-100) (STJA0010252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of DFNA5/GSDME in U251MG, U2OS, HepG2, Hela lysates using GSDME antibody.

Anti-GSDME antibody (1-270 aa) [R07-5I1] (STJA0011231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$332.78

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Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GSDME antibody (200-280 aa) (STJ96411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using GSDME Rabbit pAb (STJ29568) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSDME antibody (271-496) (STJ29568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (400-496) (STJA0010251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using [KO Validated] GSK3 Alpha Rabbit polyclonal antibody (STJ11102944) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSK3 Alpha antibody (400-483) (STJ11102944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-GSK3 Beta antibody [SRM13034] (STJ11107764)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-GSK3 Beta antibody {ABflo® 647} (STJ11109020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g GSK3 Alpha antibody (STJ11101598). Western blot was performed from the immunoprecipitate using GSK3 Alpha antibody (STJ11101598) at a dilution of 1:1000.

Anti-GSK3A antibody (1-100) [S8MR] (STJ11101598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various cells using GSK3 Alpha Polyclonal Antibody

Anti-GSK3A antibody (10-59 aa) (STJ93445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GSK3α/β (PTR2350) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 52kDaObserved band size: 42kDa

Anti-GSK3A antibody (200-300) [PTR2350] (STJA0006275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of NIH/3T3 cells using GSK3 Alpha antibody (STJ23877) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GSK3A antibody (409-483) (STJ23877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis using GSK3 Alpha monoclonal antibody against truncated GSK3 alpha recombinant protein (1) and A549 cell lysate (2).

Anti-GSK3A antibody [6G2F1/6G2F2] (STJ98117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

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Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using GSK3 Beta Mouse mAb diluted at 1:200.

Anti-GSK3B antibody (1-100 aa) [1A6] (STJ97739)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of rat-lung tissue. 1, GSK3 Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-GSK3B antibody (1-50 aa) (STJ93447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit

$64.80 - $340.20

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Western blot with Anti-GSK3B Antibody (STJ501295) of rat liver at dilution in buffer. Total IgY, B. Affinity purified GSK3b antibody

Anti-GSK3B antibody (1-50) (STJ501295)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-GSK3B antibody (1-50) {Biotin} (STJ501296)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-GSK3B antibody (1-50) {FITC} (STJ501297)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunocytochemistry analysis of GSK3 beta (2E6) in HeLa using GSK3 beta antibody.

Anti-GSK3B antibody (313-422 aa) [2E6-D6-C12] (STJA0012948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$357.08

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Immunoprecipitation analysis of 200 Mu g extracts of 22Rv1 cells, using 3 Mu g GSK3 Beta antibody (STJ27912). Western blot was performed from the immunoprecipitate using GSK3 Beta antibody (STJ27912) at a dilution of 1:1000.

Anti-GSK3B antibody (320-420) (STJ27912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of mouse brain, using GSK3 Beta antibody (STJ23879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GSK3B antibody (350-420) (STJ23879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-GSK3B antibody (N-Term) [M131] (STJA0005433)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

$431.33

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Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3 Beta mouse mAb (1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.

Anti-GSK3B antibody [2E6-D6-C12] (STJ99095)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse

$194.40 - $469.80

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Western blot analysis using GSK3 Beta monoclonal antibody against A549 (1) , K562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.

Anti-GSK3B antibody [3D10] (STJ98120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$194.40 - $469.80

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Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) (GSS) Rabbit monoclonal antibody (STJ11101358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSS antibody (350-450) [S8MR] (STJ11101358)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS) ) knockout (KO) 293T cells, using Glutathione Synthetase (Glutathione Synthetase (GSS) ) antibody (STJ116746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSS antibody (373-474) (STJ116746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-GSS antibody (STJ193061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using GTF2A1 antibody (STJ27298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-GTF2A1 antibody (1-270) (STJ27298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-GTF2A1 antibody (281-330 aa) (STJ95979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of NCI-H460 cells, using GTF2A2 antibody (STJ29993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GTF2A2 antibody (1-109) (STJ29993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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