Disease | Research Area | St John's Laboratory

Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr493) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZAP70-Tyr493 antibody (460-509 aa) (STJ90437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g Phospho-ZAP70-Y319 pAb (STJ22448). Western blot was performed from the immunoprecipitate using Phospho-ZAP70-Y319 pAb (STJ22448) at a dilution of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight.

Anti-Phospho-ZAP70-Y319 antibody (STJ22448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-ZAP70-Y493 Rabbit pAb (STJ22449) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight or treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ZAP70-Y493 antibody (STJ22449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit monoclonal antibody (STJ11105888) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-ZAP70-Y493 antibody [S5888RM] (STJ11105888)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using NIPA (Phospho-Ser354) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZC3HC1-Ser354 antibody (320-369 aa) (STJ90545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat spleen using PI3 Kinase p110 delta Rabbit monoclonal antibody (STJ11103107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PI3 Kinase p110 delta antibody (200-300) [S7MR] (STJ11103107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Mouse mAb (STJ11106530) at 1:1000 dilution. <br/> Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 30s.

Anti-PI3 Kinase p85 alpha antibody [SMM] (STJ11106530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of NIH/3T3 cells using PIK3C3/VPS34 Rabbit polyclonal antibody (STJ114357) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PIK3C3 antibody (130-412) (STJ114357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Rat brain cells using 3 Mu g PIK3C3/VPS34 antibody (STJ11101401). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (STJ11101401) at a dilution of 1:500.

Anti-PIK3C3 antibody (788-887) [S1MR] (STJ11101401)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of 293T 3T3 cells using PI 3 Kinase Class 3 Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000

Anti-PIK3C3 antibody (STJ98590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PIK3CA H1047R (ABT453) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 124kDaObserved band size: 124kDa

Anti-PIK3CA antibody (1000-1068 aa) [ABT453] (STJ197258)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded rat liver using PIK3CA Rabbit polyclonal antibody (STJ114358) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PIK3CA antibody (300-600) (STJ114358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using PIK3CA Rabbit polyclonal antibody (STJ24991) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PIK3CA antibody (401-1068) (STJ24991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of 293T cells, using PIK3CA antibody (STJ11102810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3CA antibody (401-600) (STJ11102810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of rat-lung tissue. 1, PI 3-kinase p110 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PIK3CA antibody (470-519 aa) (STJ95074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PIK3CA antibody (STJA0009923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using PI3 Kinase p110 beta Rabbit monoclonal antibody (STJ11103834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3CB antibody (1-100) [S3834RM] (STJ11103834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates, using PI3 Kinase p110 beta rabbit polyclonal antibody (STJ24994) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PIK3CB antibody (410-600) (STJ24994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PIK3CB antibody (410-600) [S4849RM] (STJ11104849)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PIK3CB antibody (STJ191945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of mouse thymus, using PI3 Kinase p110 delta antibody (STJ118711) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PIK3CD antibody (170-300) (STJ118711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HepG2 cells using PI 3-Kinase p110 Delta Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PIK3CD antibody (41-90 aa) (STJ96902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using PI3 Kinase p85 alpha rabbit monoclonal antibody (STJ11102215) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R1 antibody (1-100) [S5MR] (STJ11102215)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of U-87MG cells, using PI3 Kinase p85 alpha antibody (STJ113826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R1 antibody (240-454) (STJ113826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (STJ113725) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PIK3R1 antibody (240-724) (STJ113725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-PIK3R1 antibody (550-630 aa) (STJ99654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Western blot analysis of various cells using PI 3-kinase p85 Alpha Polyclonal Antibody diluted at 1：1000

Anti-PIK3R1 antibody (573-622 aa) (STJ95077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody, and DAPI (blue)

Anti-PIK3R1 antibody (601-619 aa) [R03-3C7] (STJA0013015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£264.50

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Anti-PIK3R1 antibody (C-Term) [M253] (STJA0005530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£394.50

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Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using PI3 Kinase P85 Rabbit pAb diluted at 1:500.

Anti-PIK3R1 antibody (STJ197340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 1) 3T3, 2) Rat LiverTissue with PI3 Kinase P85 Alpha Mouse mAb diluted at 1:2, 000.

Anti-PIK3R1 antibody [3B7] (STJ97521)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-testis, antibody was diluted at 1:100

Anti-PIK3R2 antibody (1-60 aa) (STJ98987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 using PI3 Kinase p85 beta Rabbit polyclonal antibody (STJ119550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PIK3R2 antibody (1-85) (STJ119550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 beta antibody (STJ11102171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R2 antibody (100-200) [S1MR] (STJ11102171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of PI3 Kinase p85 beta in paraffin-embedded mouse spleen using PI3 Kinase p85 beta Rabbit polyclonal antibody (STJ119610) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PIK3R2 antibody (108-298) (STJ119610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PI 3 Kinase p85 beta antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PIK3R2 antibody (140-164 aa) [R06-5H3] (STJA0014501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Rat

£264.50

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Western blot analysis of various cells using PI 3-kinase p85 Beta Polyclonal Antibody diluted at 1：500

Anti-PIK3R2 antibody (409-458 aa) (STJ95080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of PI3 Kinase p85 beta in C6, K562, MCF7, U87MG, HCT116, 3T3 and Hela cell lysates using PI3 Kinase p85 beta mouse mAb (1:1000 diluted).Predicted band size:85KDa.Observed band size:85KDa.

Anti-PIK3R2 antibody [8D9-D5-F8] (STJ99192)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of various lysates using PIK3R3/p55PIK Rabbit pAb (STJ110251) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PIK3R3 antibody (1-230) (STJ110251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using PIK3R3/p55PIK Rabbit polyclonal antibody (STJ11101208) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PIK3R3 antibody (300-388) (STJ11101208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PIK3R3 antibody (300-388) (STJ119452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PIK3R3/p55PIK Rabbit monoclonal antibody (STJ11101979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIK3R3 antibody (362-461) [S9MR] (STJ11101979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PIK3R3 antibody (STJ191947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using PIK3R4/VPS15 Rabbit polyclonal antibody (STJ118273) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PIK3R4 antibody (1-330) (STJ118273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PIK3R4 antibody (170-220) (STJA0006495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of NIH/3T3 cells using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PIK3R4/VPS15 antibody (1259-1358) [S9MR] (STJ11103589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PIK3R6 antibody (280-360 aa) (STJ191158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of 22Rv1 cells, using PIM1 antibody (STJ11101771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIM1 antibody (1-100) [S1MR] (STJ11101771)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options