Anti-MUC4 antibody (2000-2100 aa) [ABT199] (STJ197170)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2ak
ImmunogenSynthesized peptide derived from the human MUC4 at the amino acid range 2000-2100
General Information
| Short Description | Mouse monoclonal anti-Mucin-4 (2000-2100 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT199 |
| Isotype | IgG2ak |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:100-500WB 1:200-1000IF 1:100-500 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MUC4 |
| Gene ID | 4585 |
| Uniprot ID | MUC4_HUMAN |
| Immunogen | Synthesized peptide derived from the human MUC4 at the amino acid range 2000-2100 |
| Immunogen Region | 2000-2100 aa |
| Specificity | The antibody can specifically recognize human MUC4 protein, and has no cross reaction wit MUC2, MUC5AC, MUC6 proteins. |
Additional Info
| Post Translational Modifications | Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain. Mucin-4 alpha chain: Highly O-glycosylated. Mucin-4 beta chain: Is predominantly N-glycosylated. |
| Function | Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces. These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation. Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. |
| Protein Name | Mucin-4Muc-4Ascites SialoglycoproteinAsgpPancreatic Adenocarcinoma MucinTestis MucinTracheobronchial Mucin Cleaved Into - Mucin-4 Alpha ChainAscites Sialoglycoprotein 1Asgp-1 - Mucin-4 Beta ChainAscites Sialoglycoprotein 2Asgp-2 |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-977068 |
| Cellular Localisation | Mucin-4 Beta Chain: Cell MembraneSingle-Pass Membrane ProteinIsoforms Lacking The Cys-Rich RegionEgf-Like Domains And Transmembrane Region Are SecretedSecretion Occurs By Splicing Or Proteolytic ProcessingMucin-4 Alpha Chain: Cell MembraneSecretedForms A Complex With Mucin-4 Beta Chain At The Cell MembraneIsoform 3: Cell MembraneIsoform 11: SecretedIsoform 15: SecretedIsoform 17: Cell Membrane |
| Alternative Antibody Names | Anti-Mucin-4 antibodyAnti-Muc-4 antibodyAnti-Ascites Sialoglycoprotein antibodyAnti-Asgp antibodyAnti-Pancreatic Adenocarcinoma Mucin antibodyAnti-Testis Mucin antibodyAnti-Tracheobronchial Mucin Cleaved Into - Mucin-4 Alpha Chain antibodyAnti-Ascites Sialoglycoprotein 1 antibodyAnti-Asgp-1 - Mucin-4 Beta Chain antibodyAnti-Ascites Sialoglycoprotein 2 antibodyAnti-Asgp-2 antibodyAnti-MUC4 antibody |
Information sourced from Uniprot.org