Disease | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1) ) antibody (STJ118150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PLCG1 antibody (1202-1291) (STJ118150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1) ) antibody (STJ110022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PLCG1 antibody (700-800) (STJ110022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PLC gamma 1 (PLC gamma 1 (PLCG1) ) Rabbit mAb (STJ11102392) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PLCG1 antibody (710-832) [S2MR] (STJ11102392)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PLC Gamma 1 Polyclonal Antibody diluted at 1：2000

Anti-PLCG1 antibody (736-785 aa) (STJ95157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-PLCG1 antibody (N-Term) [M156] (STJA0005536)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat

£394.50

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Immunofluorescence analysis of Raw264. 7 cells using PLC gamma 2 (PLC gamma 2 (PLCG2) ) antibody (STJ25024) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PLCG2 antibody (1-300) (STJ25024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PLCG2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLCG2 antibody (1186-1235 aa) (STJ95159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded rat spleen using PLC gamma 2 (PLC gamma 2 (PLCG2) ) Rabbit monoclonal antibody (STJ11102286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PLCG2 antibody (700-800) [S6MR] (STJ11102286)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from RAW 264. 7 cells using PLC gamma 2 (PLCG2) Rabbit monoclonal antibody (STJ11105529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PLCG2 antibody (701-800) [S5529RM] (STJ11105529)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various cells using Snk Polyclonal Antibody

Anti-PLK2 antibody (291-340 aa) (STJ95720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PLK2 antibody (STJ29146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLK2 antibody (361-439) (STJ29146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Anti-PLK2 antibody (377-451 aa) (STJ98963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PML antibody (STJ11101744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PML antibody (1-100) [S4MR] (STJ11101744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PML Polyclonal Antibody diluted at 1：1000

Anti-PML antibody (11-60 aa) (STJ95166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (STJ25034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PML antibody (300-600) (STJ25034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using PML antibody (STJ11100142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PML antibody (571-611) (STJ11100142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using PMM2 antibody (STJ25035) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PMM2 antibody (1-246) (STJ25035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PMM2 antibody (STJA0007115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human colon adenocarcinoma (Lynch Syndrome) stained with anti-PMS-2 antibody. Note tumor cells are negative whereas normal glands (top) are nuclear positive.

Anti-PMS-2 antibody [ZR317] (STJ180591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£199.50 - £755.50

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Western blot analysis of various lysates using PMS2 Rabbit monoclonal antibody (STJ11102108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PMS2 antibody (1-100) [S8MR] (STJ11102108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates using PMS2 Rabbit polyclonal antibody (STJ29027) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PMS2 antibody (10-21) (STJ29027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry of mouse kidney with Anti-PMS2 primary antibody (STJ502561) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-PMS2 antibody (220-270) (STJ502561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PMS2 antibody (220-270) {Biotin} (STJ502563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PMS2 antibody (220-270) {FITC} (STJ502564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using PMS2 antibody (STJ115635) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PMS2 antibody (390-670) (STJ115635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various cells using PMS2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PMS2 antibody (461-510 aa) (STJ95169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-PMS2antibody. The HRP-conjugated anti- mouseIgG antibody was used to detect the antibody. Predicted band size: 96 kDaObserved band size: 110 kDa

Anti-PMS2 antibody (600-700 aa) [PT2116] (STJ196728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-PMS2 antibody [EPR3947] (STJ16101659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£1,332.50

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Anti-PMS2 antibody [MRQ-28] (STJ16101579)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,436.50

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Western blot analysis of lysates from wild type (WT) and PMS2 knockout (KO) HeLa cells, using [KO Validated] PMS2 Rabbit monoclonal antibody (STJ11104811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PMS2 antibody [S4811RM] (STJ11104811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemistry analysis of PMS2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200, RT, 1h.

Anti-PMS2 antibody [SM0372] (STJA0010372)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-PMS2 antibody [SM5009] (STJA0005009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PNP antibody (1-289) (STJ11100761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-PNP antibody (STJA0008231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using DNA pol Alpha Polyclonal Antibody diluted at 1：1000

Anti-POLA1 antibody (81-130 aa) (STJ92733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-POLA2 antibody (STJ190749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug POLR2A Rabbit polyclonal antibody (STJ25045 1:100). Western blot was performed from the immunoprecipitate using POLR2A Rabbit polyclonal antibody (STJ25045) at a dilition of 1:1000.

Anti-POLR2A antibody (1-1970) (STJ25045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using POLR2A antibody (STJ113726) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-POLR2A antibody (1-260) (STJ113726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using POLR2A Rabbit monoclonal antibody (STJ11103279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-POLR2A antibody (1135-1311) [S9MR] (STJ11103279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various cells using Rpb1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLR2A antibody (1585-1634 aa) (STJ95538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunocytochemistry analysis of RNA Polymerase II Subunit B1 (green) in CEM using RNA Polymerase II Subunit B1 antibody, and DAPI (blue).

Anti-POLR2A antibody (1602-1610 aa) [R06-8G7] (STJA0012190)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£264.50

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Immunohistochemistry analysis of paraffin-embedded mouse liver using POLR2B antibody (STJ27724) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-POLR2B antibody (1-260) (STJ27724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-POLR2B antibody (STJA0009573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 150 Mu g extracts of SW620 cells using 3 Mu g RPB3/POLR2C antibody (STJ25046). Western blot was performed from the immunoprecipitate using RPB3/POLR2C antibody (STJ25046) at a dilution of 1:500.

Anti-POLR2C antibody (1-275) (STJ25046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2C antibody (STJ190762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g POLR2D antibody (STJ25047). Western blot was performed from the immunoprecipitate using POLR2D antibody (STJ25047) at a dilution of 1:1000.

Anti-POLR2D antibody (1-142) (STJ25047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2D antibody (STJ190763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using POLR2E Rabbit polyclonal antibody (STJ25048). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-POLR2E antibody (1-210) (STJ25048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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