Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of various lysates using Phospho-Paxillin-Y118 Rabbit mAb (STJ11102648) at 1:1000 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PXN-Y118 antibody [S8MR] (STJ11102648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (STJ22364) at 1:1000 dilution. A-431 cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-RAC1-S71 antibody (STJ22364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RARA-Ser77 antibody (46-95 aa) (STJ90963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RARA-Ser96 antibody (STJA0009994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (STJ110827) at 1:400 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-RELA-S276 antibody (STJ110827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S311 Rabbit pAb (STJ22380) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RELA-S311 antibody (STJ22380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S468 Rabbit pAb (STJ22381) at 1:400 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RELA-S468 antibody (STJ22381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S529 Rabbit pAb (STJ11101077) at 1:500 dilution incubated overnight at 4 °C. HeLa cells were treated by TNF-Alpha (100 ng/ml) and Calyculin A (50 nM) at 37 °C for 10 minutes. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.

Anti-Phospho-RELA-S529 antibody (STJ11101077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293 cells, using Phospho-NF-kB p65/RelA-S529 Rabbit pAb (STJ22379). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RELA-S529 antibody (STJ22379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S529 Rabbit monoclonal antibody (STJ11105901) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (100 ng/ml) and Calyculin A (50 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 45 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-Phospho-RELA-S529 antibody [S5901RM] (STJ11105901)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (STJ22377) at 1:10000 dilution. HeLa cells were treated by TNF-Alpha (50 ng/ml) and Calyculin A (50 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RELA-S536 antibody (STJ22377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (STJ28018) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RELA-S536 antibody (STJ28018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-NF-kB p65/RelA-S536 Rabbit monoclonal antibody (STJ11105807) at1:2000 dilution. NIH/3T3 and C6 were treated by TNF-Alpha at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-RELA-S536 antibody [S5807RM] (STJ11105807)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit monoclonal antibody (STJ11105899) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and 200 nM CA at 37 °C for 30 minutes after serum starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-Phospho-RELA-S536 antibody [S5899RM] (STJ11105899)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, NF Kappa B-p65 (phospho Ser276) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-RELA-Ser276 antibody (249-298 aa) (STJ90347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser281) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RELA-Ser281 antibody (247-296 aa) (STJ91189)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p65 (S311) Polyclonal Antibody

Anti-Phospho-RELA-Ser311 antibody (278-327 aa) (STJ91007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p65 (S468) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RELA-Ser468 antibody (435-484 aa) (STJ90348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p65 (S529) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-RELA-Ser529 antibody (496-545 aa) (STJ90350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of human-lung tissue. 1, NF Kappa B-p65 (phospho Ser529) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-RELA-Ser529 antibody (STJ90349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, NF Kappa B-p65 (phospho Ser536) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-RELA-Ser536 antibody (502-551 aa) (STJ90351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-kB p65/RelA-T434 pAb (STJ11100979) at 1:1000 dilution or RelA antibody (STJ1177811). NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5min.

Anti-Phospho-RELA-T434 antibody (STJ11100979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of U-2 OS, using Phospho-NF-kB p65/RelA-T505 Rabbit polyclonal antibody (STJ11105817) at 1:1000 dilution. U-2 OS cells were treated by cisplatin at 37 °C for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-RELA-T505 antibody (STJ11105817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-NF Kappa B-p65 (T254) Polyclonal Antibody

Anti-Phospho-RELA-Thr254 antibody (221-270 aa) (STJ90352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr435) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RELA-Thr435 antibody (402-451 aa) (STJ90353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr505) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RELA-Thr505 antibody (471-520 aa) (STJ91119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using Ret (Phospho-Tyr1015) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RET-Tyr1015 antibody (991-1040 aa) (STJ90478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of Ret (phospho Tyr1062) Polyclonal Antibody, using HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-RET-Tyr1062 antibody (1041-1090 aa) (STJ90679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of K562 using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-RET-Tyr1096 antibody (STJA0010080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-RET-Tyr905 antibody (881-930 aa) (STJ91308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Ret-Y1062 antibody (STJ11107656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from K562 cells, using Phospho-Ret-Y905 Rabbit pAb (STJ29366). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Ret-Y905 antibody (STJ29366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western Blot analysis of mouse kidney, rat kidney. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJ99666 was diluted at 1:10000

Anti-Phospho-RHOA-Ser188 antibody (STJA0009821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, using Phospho-RIPK3-S232 Rabbit pAb (STJ11107688) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-RIPK3-S232 antibody (STJ11107688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western Blot analysis of mouse spleen /Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ROCK2-Tyr722 antibody (STJA0009928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Rpb1 CTD-S2/S5-S2/S5 antibody [SRM15121] (STJ11109851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ChIP/CUT&Tag/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-Rpb1-Ser2 antibody [PT1003R] (STJA0039928)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-Rpb1-Ser5 antibody [PT0923R] (STJA0039821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from HeLa cells using Phospho-S6 Ribosomal Protein (RPS6) -S235 Rabbit pAb (STJ11105772) at 1:800 dilution. HeLa cells were treated by 20% FBS at 37 °C for 90 minutes after serum starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RPS6-S235 antibody (STJ11105772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-RPS6-S235-S235 antibody [SRM15136] (STJ11109866)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S235/236 Rabbit pAb (STJ113489) at 1:1000 dilution. HeLa cells NIH/3T3 cells and PC-12 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6-S235/236 antibody (STJ113489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit pAb (STJ113488) at 1:1000 dilution or RPS6 antibody (STJ27852). HeLa and NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6-S240/244 antibody (STJ113488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293 cells treated with serum 10% 15', using S6 Ribosomal Protein (Phospho-Ser235) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser235 antibody (200-249 aa) (STJ90403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody. The lane on the right is blocked with the S6 Ribosomal Protein (Phospho-Ser235+Ser236) peptide.

Anti-Phospho-RPS6-Ser235/S236 antibody (200-249 aa) (STJ90992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using S6 Ribosomal Protein (Phospho-Ser240) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser240 antibody (200-249 aa) (STJ91053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-RPS6-Ser240/244 antibody [PT0447R] (STJA0039213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using (STJ116387) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6KA1-S363 antibody (STJ116387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (STJ113499) at 1:1000 dilution or RPS6KA1 antibody (STJ240768). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6KA1-S380 antibody (STJ113499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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