Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of various lysates, using PSMF1 rabbit polyclonal antibody (STJ27500) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSMF1 antibody (1-271) (STJ27500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-PSMF1 antibody (STJA0008039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 293 cells using PSP Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PSPN antibody (101-150 aa) (STJ96660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PTF1A antibody (172-222 aa) (STJ195806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-PTGDS antibody (115-165) (STJA0006385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of Mouse heart, using PTGDS antibody (STJ27680) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTGDS antibody (23-190) (STJ27680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-PTK2 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PTK2 antibody (690aa C-Term) (STJ140154)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€169.38 - €493.13

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Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit polyclonal antibody (STJ25213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (700-800) (STJ25213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Confocal imaging of HeLa cells using FAK Rabbit monoclonal antibody (STJ112977, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-PTK2 antibody (700-800) [S7MR] (STJ112977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit polyclonal antibody (STJ113175) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (734-834) (STJ113175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using FAK Rabbit polyclonal antibody (STJ114921) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (734-834) (STJ114921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry of rat tissue with Anti-PTK2 primary antibody (STJ500981) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-PTK2 antibody (740-790) (STJ500981)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-PTK2 antibody (740-790) {Biotin} (STJ500982)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-PTK2 antibody (740-790) {FITC} (STJ500983)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunofluorescence analysis of Hela cell. 1, FAK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PTK2 antibody (809-858 aa) (STJ93024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis using FAK monoclonal antibody against A549 (1) and NIH/3T3 (2) cell lysate.

Anti-PTK2 antibody [10H7] (STJ98061)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€180.00 - €435.00

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Western blot analysis using FAK monoclonal antibody against FAK-hIgGFc transfected HEK293 cell lysate.

Anti-PTK2 antibody [10H7A6] (STJ98062)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Anti-PTK2 antibody [10H7E9] (STJ98063)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot detection of FAK in COS7, HUVEC, A549 and Hela cell lysates using FAK mouse mAb (1:1000 diluted).Predicted band size: 125KDa.Observed band size:125KDa.

Anti-PTK2 antibody [3D8-H11-E12] (STJ99191)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis using FAK monoclonal antibody against truncated FAK recombinant protein.

Anti-PTK2 antibody [4A9D6] (STJ98060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Confocal imaging of NIH/3T3 cells using SHP2 Rabbit monoclonal antibody (STJ11101649, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PTPN11 antibody (1-100) [S9MR] (STJ11101649)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g SHP2 antibody (STJ114360). Western blot was performed from the immunoprecipitate using SHP2 antibody (STJ114360) at a dilution of 1:2000.

Anti-PTPN11 antibody (1-460) (STJ114360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1：2000

Anti-PTPN11 antibody (321-370 aa) (STJ95660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1：1000

Anti-PTPN11 antibody (508-557 aa) (STJ95659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of PC-12 cells using SHP2 Rabbit polyclonal antibody (STJ25224) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTPN11 antibody (514-593) (STJ25224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-PTPN11 antibody (546-595 aa) (STJ95658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-PTPN11 antibody (N-Term) [M163] (STJA0005583)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€399.38

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Western blot analysis of various lysates using PTPRA Rabbit polyclonal antibody (STJ114202) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTPRA antibody (175-295) (STJ114202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human stomach using PTPRA Antibody (STJ25230) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PTPRA antibody (20-142) (STJ25230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-PTPRA antibody (650-700) (STJ502720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-PTPRA antibody (650-700) {Biotin} (STJ502721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-PTPRA antibody (650-700) {FITC} (STJ502722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PTPRA antibody (730-810 aa) (STJ95267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of paraffin-embedded human tonsil using Human CD45RA Rabbit polyclonal antibody (STJ11105482) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-PTPRC antibody (10-110) (STJ11105482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human

€140.63 - €655.63

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Endogenous CD45 detected with at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-PTPRC antibody (1260aa C-Term) (STJ140026)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€108.13 - €668.13

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Immunofluorescence analysis of Jurkat cells using CD45 Rabbit polyclonal antibody (STJ25235) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTPRC antibody (194-1306) (STJ25235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD45 Rabbit monoclonal antibody (STJ11101559) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PTPRC antibody (194-390) [S9MR] (STJ11101559)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€161.88 - €718.13

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Immunofluorescence analysis of paraffin-embedded Rat spleen using Human CD45RO Rabbit polyclonal antibody (STJ11105481) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PTPRC antibody (27-120) (STJ11105481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Rat

€140.63 - €655.63

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Jurkat whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD45 (ABT-CD45) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 200kDaObserved band size: 200kDa

Anti-PTPRC antibody (500-600 aa) [ABT-CD45] (STJ196813)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF/FC

Reactivity :

Human

€217.50 - €315.00

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Anti-PTPRC antibody (500-600 aa) [PT1659] (STJ196748)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

€217.50 - €315.00

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Western blot analysis of extracts of Jurkat cells, using CD45 antibody (STJ25232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PTPRC antibody (611-1306) (STJ25232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of Jurkat cells using CD45 Polyclonal Antibody

Anti-PTPRC antibody (981-1030 aa) (STJ92130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of human-liver-cancer tissue. 1, CD45 monoclonal antibody (12A9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PTPRC antibody [12A9] (STJ96948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human

€60.00 - €315.00

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Western blot analysis using CD45 monoclonal antibody against truncated CD45 recombinant protein (1).

Anti-PTPRC antibody [4A8A4C7A2] (STJ97930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

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Immunofluorescence analysis of NIH-3T3 cells using Rac1/2/3 Rabbit polyclonal antibody (STJ110031) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RAC1 antibody (1-192) (STJ110031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from rat brain, using Rac1/2/3 Rabbit polyclonal antibody (STJ11104542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RAC1 antibody (1-192) (STJ11104542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-RAC1+RAC2 antibody (100-150 aa) (STJ13100299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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Western blot analysis of extracts of various cell lines, using Radixin Rabbit monoclonal antibody (STJ11103113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Radixin antibody (350-450) [S3MR] (STJ11103113)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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