Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using NFASC antibody (STJ24750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NFASC antibody (830-1100) (STJ24750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-NFASC antibody (STJA0007413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 1) MCF7 Cell, 2) Mouse Brain Tissue lysate using NF κB P105/P50 mouse Monoclonal monoclonal antibody diluted at 1:1,000.

Anti-NFKB P105/P50 antibody [4D11] (STJ196682)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse

$234.90 - $340.20

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Western blot analysis of MCF7 Cell lysate using NF κB P50 mouse Monoclonal monoclonal antibody diluted at 1:1,000.

Anti-NFKB P50 antibody [5D2] (STJ196683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

$234.90 - $340.20

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Immunofluorescence analysis of Hela cell. 1, NF Kappa B-p105/p50 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-NFKB1 antibody (304-353) (STJ94466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of mouse lung, using NFKB1 antibody (STJ28750) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-NFKB1 antibody (41-365) (STJ28750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of BALB-3T3 cells using NFKB1 Rabbit polyclonal antibody (STJ116954) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFKB1 antibody (41-968) (STJ116954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 150 Mu g extracts of MCF-7 cells using 3 Mu g NFKB1 antibody (STJ113719). Western blot was performed from the immunoprecipitate using NFKB1 antibody (STJ113719) at a dilution of 1:500.

Anti-NFKB1 antibody (740-964) (STJ113719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various cells using NF Kappa B-p105 Polyclonal Antibody

Anti-NFKB1 antibody (896-945) (STJ94463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis using NF Kappa B-p105/p50 monoclonal antibody against K562 (1) , Jurkat (2) , A431 (3) , HeLa (4) , THP-1 (5) and MCF-7 (6) cell lysate.

Anti-NFKB1 antibody [5D10] (STJ98275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Anti-NFKB1 antibody [5D10D11] (STJ98274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot detection of NF-Kappa B1 p105/p50 in K562, C6, 3T3 and Hela cell lysates using NF-Kappa B1 p105/p50 mouse mAb (dilution 1:500).Predicted band size:120, 50kDa.Observed band size:120, 50kDa.

Anti-NFKB1 antibody [5E3-E4-C11] (STJ99314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of various lysates using NGEF Rabbit polyclonal antibody (STJ118934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-NGEF antibody (429-710) (STJ118934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-NGEF antibody (STJA0008709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NKX2-2 using NKX2-2 Rabbit polyclonal antibody (STJ11105621) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NKX2-2 antibody (102-201) (STJ11105621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunohistochemistry of rat brain with Anti-NKX2-2 primary antibody (STJ501927) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-NKX2-2 antibody (200-250) (STJ501927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-NKX2-2 antibody (200-250) {Biotin} (STJ501929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-NKX2-2 antibody (200-250) {FITC} (STJ501930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-NKX2-2 antibody (200-273) (STJ501928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-NKX2-2 antibody (200-273) {Biotin} (STJ501931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-NKX2-2 antibody (200-273) {FITC} (STJ501932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NKX2-2 antibody (STJ190894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using NKX2-5 antibody (STJ27618) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-NKX2-5 antibody (1-135) (STJ27618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis using Nkx-2.5 monoclonal antibody against full-length NKX2.5 (aa1-324) -hIgGFc transfected HEK293 cell lysate (1).

Anti-NKX2-5 antibody [2E1] (STJ98278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Human Ewing NA s sarcoma stained with anti-NKX2. 2 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Anti-NKX2.2 antibody (1-119aa) [ZM14] (STJ180293)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$222.08 - $812.03

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Immunohistochemistry analysis of paraffin-embedded mouse pancreas using NKX6-1 antibody (STJ11103302) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NKX6-1 antibody (1-100) (STJ11103302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of NKX61 Antibody. The lane on the right is blocked with the NKX61 peptide.

Anti-NKX6-1 antibody (180-260) (STJ94508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-NMDAR1 antibody (ECD) (STJ13100195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

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Western blot analysis of various lysates using NODAL Rabbit pAb (STJ111943) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-NODAL antibody (198-347) (STJ111943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Western blot analysis of Hela lysis using NODAL antibody.. Secondary antibody was diluted at 1:20000

Anti-NODAL antibody (294-343 aa) (STJ97680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-NOG antibody (21-70 aa) (STJ98814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-NOG antibody (Internal Region) (STJA0005795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$614.93

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Western blot detection of Noggin in CHO-K1 cell lysates over-expressing Noggin-PDGFR transmembrane domain fused protein using Noggin mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:37KDa.

Anti-NOG antibody [1D2-G8-G1] (STJ99187)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of lysates from A-549 cells, using Non-phospho-YAP1-S127 Rabbit pAb (STJ11103239) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Non-phospho-YAP1-S127 antibody (50-150) (STJ11103239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of Jurkat cells, using Notch1 antibody (STJ11100238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NOTCH1 antibody (1567-1729) (STJ11100238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using Notch1 Rabbit pAb (STJ119092) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-NOTCH1 antibody (1715-1814) (STJ119092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using Notch1 Rabbit polyclonal antibody (STJ11103863) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NOTCH1 antibody (2381-2480) (STJ11103863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of Rat brain cells, using Notch1 antibody (STJ29951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NOTCH1 antibody (2456-2555) (STJ29951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g Notch1 antibody (STJ11101627). Western blot was performed from the immunoprecipitate using Notch1 antibody (STJ11101627) at a dilution of 1:1000.

Anti-NOTCH1 antibody (2456-2555) [S7MR] (STJ11101627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Western blot analysis of extracts of U-251MG cells, using Notch1 antibody (STJ119090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-NOTCH1 antibody (2504-2518) (STJ119090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NPNT antibody (485-535 aa) (STJ193586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates, using NR2F2 antibody (STJ112289) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR2F2 antibody (1-100) (STJ112289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using ARP-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NR2F2 antibody (1-50 aa) (STJ91700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using Steroidogenic factor-1 (SF-1/NR5A1) Rabbit polyclonal antibody (STJ28057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR5A1 antibody (1-120) (STJ28057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from rat lung, using STF-1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NR5A1 antibody (169-218 aa) (STJ95626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of Rat brain, using SF-1 Rabbit polyclonal antibody (STJ11105403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NR5A1 antibody (218-461) (STJ11105403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$151.88 - $708.08

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Human adrenal gland stained with anti-SF-1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of cortical cells.

Anti-NR5A1 antibody (220-461aa) [N1665] (STJ180239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$315.23 - $1,489.73

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Western blot analysis of GTPase HRAS in 3T3, mouse brain lysates using GTPase HRAS antibody.

Anti-NRAS antibody (27-40 aa) [R01-7D0] (STJA0012568)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Hamster

$357.08

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