Anti-EDN1 antibody (131-180 aa) (STJ96512)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human EDN1 at the amino acid range 131-180
General Information
| Short Description | Rabbit polyclonal anti-Endothelin-1 (131-180 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC-P 100-300WB 1:500-1:2000ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EDN1 |
| Gene ID | 1906 |
| Uniprot ID | EDN1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1 at the amino acid range 131-180 |
| Immunogen Region | 131-180 aa |
| Specificity | ET-1 Polyclonal Antibody detects endogenous levels of ET-1 protein. |
Additional Info
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2. |
| Protein Name | Endothelin-1Preproendothelin-1Ppet1 Cleaved Into - Endothelin-1Et-1 - Big Endothelin-1 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-9856649 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Endothelin-1 antibodyAnti-Preproendothelin-1 antibodyAnti-Ppet1 Cleaved Into - Endothelin-1 antibodyAnti-Et-1 - Big Endothelin-1 antibodyAnti-EDN1 antibody |
Information sourced from Uniprot.org