Developmental Biology | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using WNT3A antibody (STJ115562) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-WNT3A antibody (1-352) (STJ115562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Western blot analysis of U2OS, using WNT3A antibody (STJ26114) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-WNT3A antibody (139-155) (STJ26114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot detection of Wnt3a in MCF-7, ZR75-1, MDA-MB-468 and T47D cell lysates using Wnt3a mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size:42KDa.

Anti-WNT3A antibody [6H11-H11-G7] (STJ99281)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis of extracts of various cell lines, using WNT4 antibody (STJ110119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-WNT4 antibody (122-351) (STJ110119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-WNT4 antibody (190-270 aa) (STJ190389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-WNT9B antibody (120-200 aa) (STJ190395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of OVCAR-3 cells using WT1 Rabbit polyclonal antibody (STJ118729) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-WT1 antibody (1-302) (STJ118729)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using WT1 antibody (STJ26120) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WT1 antibody (1-302) (STJ26120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-WT1 antibody (310-390 aa) (STJ192378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-WT1 (ABT-WT1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 55kDaObserved band size: 55kDa

Anti-WT1 antibody (350-449 aa) [ABT-WT1] (STJ196897)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

€217.50 - €315.00

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Western blot analysis of extracts of various cell lines, using WT1 antibody (STJ11103494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-WT1 antibody (60-250) [S4MR] (STJ11103494)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Anti-WT1 antibody [6F-H2] (STJ16100439)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

€865.63 - €1,053.13

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Human ovarian adenocarcinoma stained with anti-WT-1 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Anti-WT1 antibody [6F‐H2] (STJ180267)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

€201.88 - €656.88

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Western blot analysis of various lysates, using TAZ Rabbit monoclonal antibody (STJ11104982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-WWTR1 antibody (1-270) [S4982RM] (STJ11104982)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of lysates from Mouse lung, using TAZ Rabbit pAb (STJ110501) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-WWTR1 antibody (1-400) (STJ110501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using TAZ antibody (STJ118251) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-WWTR1 antibody (300-400) (STJ118251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-WWTR1 antibody (STJ191805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using Y14/RBM8A Rabbit monoclonal antibody (STJ11103049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Y14/RBM8A antibody (1-100) [S9MR] (STJ11103049)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of YAP1 in mouse embryo using YAP1 antibody.

Anti-YAP1 antibody (117-132 aa) [R06-5E9] (STJA0012146)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

€330.63

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Immunofluorescence analysis of HeLa cells using YAP1 Rabbit polyclonal antibody (STJ11104289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-YAP1 antibody (155-504) (STJ11104289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of YAP1 in paraffin-embedded human colon using [KO Validated] YAP1 Rabbit polyclonal antibody (STJ11104506) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-YAP1 antibody (155-504) (STJ11104506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€161.88 - €803.13

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g YAP1 antibody (STJ113749). Western blot was performed from the immunoprecipitate using YAP1 antibody (STJ113749) at a dilution of 1:1000.

Anti-YAP1 antibody (155-504) (STJ113749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Immunofluorescence analysis of PC-12 cells using YAP1 antibody (STJ113750) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-YAP1 antibody (155-504) (STJ113750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts from HeLa cells, using YAP1 rabbit polyclonal antibody (STJ26137) at 1:20000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer:3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YAP1 antibody (155-504) (STJ26137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunofluorescence analysis of NIH/3T3 cells using YAP1 Rabbit polyclonal antibody (STJ29831) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-YAP1 antibody (155-504) (STJ29831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of C6 cells, using YAP1 antibody (STJ11101671) at1:4000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YAP1 antibody (155-504) [S1MR] (STJ11101671)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunofluorescence analysis of U-2 OS cells using YAP1 Rabbit polyclonal antibody (STJ119295) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-YAP1 antibody (276-488) (STJ119295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of Hela cell. 1, YAP Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-YAP1 antibody (281-330 aa) (STJ96289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-YAP1 antibody (300-350) (STJ506329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€534.38

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Anti-YAP1 antibody (300-350) {Biotin} (STJ506330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€660.63

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Anti-YAP1 antibody (300-350) {FITC} (STJ506331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€660.63

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using YAP1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-YAP1 antibody (383-396 aa) [R06-6H7] (STJA0012147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

€330.63

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Immunofluorescence analysis of U2OS cells using YES1 Rabbit polyclonal antibody (STJ29810) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-YES1 antibody (1-250) (STJ29810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis using c-Yes monoclonal antibody against truncated YES1-His recombinant protein (1) and full-length GFP-YES1 (aa1-543) transfected COS7 cell lysate (2).

Anti-YES1 antibody (10-193 aa) [2F3E6] (STJ97980)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot with Anti-YES1 Antibody (STJ503564) on mouse lung. Antibody at 1:250 dilution in buffer.

Anti-YES1 antibody (450-480) (STJ503564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€534.38

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Anti-YES1 antibody (450-480) {Biotin} (STJ503565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Anti-YES1 antibody (450-480) {FITC} (STJ503566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€660.63

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (STJ11101704) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-YY1 antibody (200-300) [S4MR] (STJ11101704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-YY1 antibody (230-310 aa) (STJ192391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (STJ114794) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-YY1 antibody (80-160) (STJ114794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of H9C2 cells using ZCCHC11 Rabbit polyclonal antibody (STJ116288) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ZCCHC11 antibody (1-290) (STJ116288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded mouse brain using ZEB2 Rabbit polyclonal antibody (STJ29919) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ZEB2 antibody (1050-1214) (STJ29919)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using SIP1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ZEB2 antibody (71-120 aa) (STJ95665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using TIS11D Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ZFP36L2 antibody (207-256 aa) (STJ96034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot with Anti-ZFP36L2 Antibody (STJ503576) on mouse large intestine at 1:150 dilution in 1% milk.

Anti-ZFP36L2 antibody (350-400) (STJ503576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-ZFP36L2 antibody (350-400) {Biotin} (STJ503577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-ZFP36L2 antibody (350-400) {FITC} (STJ503578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Western blot analysis of Hela AD293 293T 453 HepG2 lysis using ZFPM2 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ZFPM2 antibody (921-970 aa) (STJ97688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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