Anti-NKX2-5 antibody [2E1] (STJ98278)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human Nkx-2.5 expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Homeobox protein Nkx-2.5 for use in WB and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2E1 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Antibody are purified by protein G affinity chromatography. |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in 0.01M PBS pH7.4 containing 0.03% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NKX2-5 |
| Gene ID | 1482 |
| Uniprot ID | NKX25_HUMAN |
| Immunogen | Purified recombinant fragment of human Nkx-2.5 expressed in E. Coli. |
| Specificity | Nkx-2.5 Monoclonal Antibody detects endogenous levels of Nkx-2.5 protein. |
Additional Info
| Function | Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4. May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal. Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression. Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia. |
| Protein Name | Homeobox Protein Nkx-2.5Cardiac-Specific HomeoboxHomeobox Protein CsxHomeobox Protein Nk-2 Homolog E |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-5578768Reactome: R-HSA-9733709 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nkx-2.5 antibodyAnti-Cardiac-Specific Homeobox antibodyAnti-Homeobox Protein Csx antibodyAnti-Homeobox Protein Nk-2 Homolog E antibodyAnti-NKX2-5 antibodyAnti-CSX antibodyAnti-NKX2.5 antibodyAnti-NKX2E antibody |
Information sourced from Uniprot.org