Phospho Antibodies | St John's Laboratory

Immunofluorescence analysis of mouse-liver tissue. 1, ERK 1/2 (phospho Thr202) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202 antibody (169-218 aa) (STJ90260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Erk 1/2-Thr202/Tyr204 antibody [PT0643R] (STJA0039299)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunofluorescence analysis of rat-lung tissue. 1, ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Thr202/Y204 antibody (STJ91357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish

$67.62 - $360.18

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Immunofluorescence analysis of rat-spleen tissue. 1, ERK 1/2 (phospho Tyr204) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-ERK 1/2-Tyr204 antibody (170-219 aa) (STJ90261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-tubulin monoclonal antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-ERK-Tyr222/205 antibody (STJ90657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot (WB): The picture was kindly provided by our customer. The primary antibody was diluted at 1:2000. The STJ97738

Anti-Phospho-ERK-Y222/205 antibody (140-220 aa) [4G3] (STJ97738)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot A431 cells ERK2 phosphorylation with calyculin A and phosphatase treatment STJA0041209

Anti-Phospho-ERK1 (T207) /ERK2 (T188)-Thr-188 antibody (STJA0041209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101064)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

$445.05

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

$445.05

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

$445.05

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Anti-Phospho-Erk1 + Erk2-T202/Y204 antibody (STJ13101067)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

$445.05

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Western blot A431 cells ERK1-2 phosphorylation detection with calyculin A and EGF treatment STJA0041190

Anti-Phospho-ERK1, ERK2-Thr-202/Tyr-204 antibody [M206] (STJA0041190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$799.71

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Phospho-ERK1/2 antibody western blot HeLa TPA lysates STJA0033905

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R05-2H9] (STJA0033905)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Hamster/Rat

$184.23 - $388.47

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Phospho-ERK1/2 antibody western blot HeLa C6 cells STJA0033913

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R07-3G4] (STJA0033913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Phospho-ERK1/2 antibody western blot NIH/3T3 lysates STJA0035674

Anti-Phospho-ERK1/2-Thr202/Thr185 antibody [R49-1Y-4] (STJA0035674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb (201245, 1:500 diluted, upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246, 1:500 diluted, lower).

Anti-Phospho-ERK1/2-Thr202/Tyr204 antibody [2E8-2F6-C12] (STJ99286)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

$205.62 - $496.80

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phospho-ERK1/2 antibody western blot A431 lysates EGF STJA0035749

Anti-Phospho-ERK1/2-Thr202/Tyr204+Thr185/Tyr187 antibody [R08-8S3] (STJA0035749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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Phospho-ERK1/2 antibody immunohistochemistry human colon carcinoma STJA0032931

Anti-Phospho-ERK1/2-Tyr222/Tyr205 antibody [10C8-9F6-10C8] (STJA0032931)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Phospho-ERK1/2 antibody immunohistochemistry human colon carcinoma STJA0032903

Anti-Phospho-ERK1/2-Tyr222/Tyr205 antibody [1H4-6D7-9D8] (STJA0032903)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Phospho-ERK1/2 antibody western blot C6 HeLa lysates STJA0034288

Anti-Phospho-ERK1/2/-Thr202/Tyr204+Thr185/Tyr187 antibody [R04-9E9] (STJA0034288)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (STJ11102556) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ERK1/ERK2-T202/T185 antibody [S6MR] (STJ11102556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IRE1-S724 Rabbit pAb (STJ11105788) at 1:800 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-ERN 1-S724 antibody (STJ11105788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various cells using Phospho-Separase (S801) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ESPL1-Ser801 antibody (767-816 aa) (STJ91032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from MCF7 cells, using Phospho-ESR Alpha-S106 Rabbit pAb (STJ22134). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S106 antibody (STJ22134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of lysates from MCF7 cells, using Phospho-ESR Alpha-S118 pAb (STJ22133) at 1:1000 dilution. MCF7 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ESR1-S118 antibody (STJ22133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ESR Alpha-S118 Rabbit mAb (STJ11102593) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-ESR1-S118 antibody [S3MR] (STJ11102593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$198.03 - $853.53

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Western blot analysis of lysates from MCF7 cells using Phospho-ESR Alpha-S167 Rabbit pAb (STJ22135). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S167 antibody (STJ22135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.57 - $853.53

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser102) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser102 antibody (71-120 aa) (STJ91254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-ER Alpha (S104) Polyclonal Antibody

Anti-Phospho-ESR1-Ser104 antibody (71-120 aa) (STJ90262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of RAT-BRAIN cells using Phospho-ER Alpha (S106) Polyclonal Antibody diluted at 1：500

Anti-Phospho-ESR1-Ser106 antibody (71-120 aa) (STJ90263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-ER Alpha (S118) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-ESR1-Ser118 antibody (91-140 aa) (STJ90264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser167) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser167 antibody (136-185 aa) (STJ90265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using Estrogen Receptor-alpha (Phospho-Ser305) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser305 antibody (276-325 aa) (STJ91336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western Blot analysis of 1 HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ESR1-Thr311 antibody (STJA0010024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-ESR1-Tyr-537 antibody (STJA0005415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$504.39

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Anti-Phospho-ESR1-Tyr-537 antibody [M545] (STJA0005416)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$504.39

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Western blot analysis of 293 cells using Phospho-ER Alpha (Y537) Polyclonal Antibody

Anti-Phospho-ESR1-Tyr537 antibody (501-550 aa) (STJ90808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of HepG2 cells using Phospho-ER Beta (S105) Polyclonal Antibody

Anti-Phospho-ESR2-Ser105 antibody (71-120 aa) (STJ90713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ESR2-Ser87 antibody (53-102 aa) (STJ91236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Phospho-ER alpha S118 antibody western blot MCF-7 STJA0036000

Anti-Phospho-Estrogen Receptor alpha-Ser118 antibody [R03-3T5] (STJA0036000)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-Estrogen Receptor Alpha-Tyr-537 antibody (STJA0041221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Estrogen Receptor Alpha-Tyr-537 antibody [M545] (STJA0041199)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ETS1-Ser251 antibody (STJ91277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Ets-1 (T38) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ETS1-Thr38 antibody (11-60 aa) (STJ91038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from C6 cells, using Phospho-EZH2/KMT6-T311 Rabbit pAb (STJ11105805) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-EZH2-T311 antibody (STJ11105805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$174.57 - $853.53

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Anti-Phospho-EZH2-Thr311 antibody (STJ196302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from MDA cells, using Phospho-Ezrin-T567 Rabbit pAb (STJ22136). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-EZR-T567 antibody (STJ22136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of HELA COLO using Phospho-Ezrin (T566) antibody. Antibody was diluted at 1:1000

Anti-Phospho-EZR-Thr566 antibody (534-583 aa) (STJ90266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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