• Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Separase (S801) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ESPL1-Ser801 antibody (767-816 aa) (STJ91032)

SKU:
STJ91032

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Separin-Ser801 (767-816 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ESPL1
Gene ID: 9700
Uniprot ID: ESPL1_HUMAN
Immunogen Region: 767-816 aa
Specificity: Phospho-Separase (S801) Polyclonal Antibody detects endogenous levels of Separase protein only when phosphorylated at S801.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SEPARASE around the phosphorylation site of Ser801 at the amino acid range 767-816
Function Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Protein Name Separin
Caspase-Like Protein Espl1
Extra Spindle Poles-Like 1 Protein
Separase
Database Links Reactome: R-HSA-2467813
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Separin antibody
Anti-Caspase-Like Protein Espl1 antibody
Anti-Extra Spindle Poles-Like 1 Protein antibody
Anti-Separase antibody
Anti-ESPL1 antibody
Anti-ESP1 antibody
Anti-KIAA0165 antibody

Information sourced from Uniprot.org

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