Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from COS7 cells treated with Calyculin 50nM 30', using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ACLY-Ser455 antibody (420-469 aa) (STJ90846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of IREB1 (Phospho-Ser138) Antibody. The lane on the right is blocked with the IREB1 (Phospho-Ser138) peptide.

Anti-Phospho-ACO1-Ser138 antibody (106-155 aa) (STJ91163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of 293 cells using Phospho-IRP-1 (S711) Polyclonal Antibody

Anti-Phospho-ACO1-Ser711 antibody (681-730 aa) (STJ90914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Anti-Phospho-ACTB-Tyr-53 antibody (STJA0005242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€438.60

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Actin-Alpha/Gamma-Tyr55/53 antibody (21-70 aa) (STJ91137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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WB analysis Actin antibody in mouse C2C12 cells with pervanadate treatment STJA0041066

Anti-Phospho-Actin-Tyr-53 antibody (STJA0041066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€651.00

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Anti-Phospho-ACTN4-Tyr-4 antibody (N-Term) (STJA0005246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€438.60

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Anti-Phospho-ACTR2-T237/T238 antibody (STJA0005267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€438.60

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Western blot analysis of lysates from K562 cells treated with UV 5', using ADAM17 (Phospho-Thr735) Antibody. The lane STJ90495

Anti-Phospho-ADAM17-Thr735 antibody (701-750 aa) (STJ90495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Thr445) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADD1-Thr445 antibody (411-460 aa) (STJ91115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Adducin Alpha/Beta (S726/713) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-Adducin Alpha/Beta-Ser726/713 antibody (688-737 aa) (STJ91014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADRB2-Ser346 antibody (321-370 aa) (STJ91102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor B2 (Phospho-Ser355+Ser356) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ADRB2-Ser355/S356 antibody (331-380 aa) (STJ90872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Anti-Phospho-AGO2-Ser-387 antibody (STJA0005264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€438.60

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Anti-Phospho-AGO2-Tyr-393 antibody (STJA0005265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€438.60

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Western blot analysis of HepG2 cells using Phospho-Ah Receptor (S36) Polyclonal Antibody

Anti-Phospho-Ah Receptor-Ser36 antibody (2-51 aa) (STJ90873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-AHNAK-Ser5782 antibody (STJA0009835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-AIFM1-Ser-116 antibody (STJA0005249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€438.60

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WB analysis Jurkat cells AIFM1 phospho-specific antibody detection STJA0041080

Anti-Phospho-AIFM1-Ser-116 antibody (STJA0041080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

€651.00

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Western blot analysis of lysates from HeLa cells treated with Hu 2nM 24h, using AIRE (Phospho-Ser156) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AIRE-Ser156 antibody (126-175 aa) (STJ90618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-Akt antibody [LP18] (STJ16101675)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

€822.60

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Anti-Phospho-AKT-S473 antibody (STJ13100564)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

€387.00

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Anti-Phospho-AKT-S473 antibody (STJ13100570)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

€387.00

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Immunofluorescence analysis of rat-liver tissue. 1, Akt (phospho Ser473) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Ser473 antibody (431-480 aa) (STJ90166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

€58.80 - €313.20

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Anti-Phospho-Akt-Ser473 antibody [M114] (STJA0041048)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP

Reactivity :

Human/Mouse/Rat

€651.00

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Anti-Phospho-Akt-Ser473 antibody [PT0470R] (STJA0039543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Phospho-AKT Ser473 antibody western blot C6 3T3 HeLa STJA0033931

Anti-Phospho-AKT-Ser473 antibody [R01-4H6] (STJA0033931)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Phospho-AKT Ser473 antibody western blot NIH/3T3 STJA0036228

Anti-Phospho-AKT-Ser473 antibody [R06-7C5] (STJA0036228)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of various lysates using Phospho-Akt-T308 Rabbit mAb (STJ11103774) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-Akt-T308 antibody [S4MR] (STJ11103774)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€172.20 - €742.20

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Western blot WB analysis A431 cells Akt phosphorylation at Thr-34 STJA0041062

Anti-Phospho-Akt-Thr-34 antibody (STJA0041062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Rabbit/Rat

€651.00

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Immunofluorescence analysis of human-lung tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Thr308 antibody (STJ90750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

€58.80 - €313.20

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Anti-Phospho-Akt-Thr308 antibody [PT0722R] (STJA0039210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using AKT1/2/3 (Phospho-Tyr315/316/312) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Akt-Tyr315 antibody (281-330 aa) (STJ91195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded Human brain using Phospho-Akt-Y315 Rabbit pAb (STJ11105776) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-Akt-Y315 antibody (STJ11105776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (STJ11103745) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1 & AKT2 & AKT3-S473 & S474 & S472 antibody [S5MR] (STJ11103745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from 293T cells, using Phospho-AKT1-S129 Rabbit pAb (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-Phospho-AKT1-S212 antibody [SAA0279] (STJA0019075)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

€234.60 - €1,583.40

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Western blot analysis of lysates from Jurkat cells, using Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb (STJ11103779) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S308 & S309 & S305 antibody (STJ11103779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western Blot (WB): Xiao, Jun, et al. "Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt-SREBP-2 signaling STJ97727

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€58.80 - €313.20

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Western blot analysis of lysates from C6 cells using Phospho-AKT1-S473 Rabbit pAb (STJ116369) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-AKT1-S473 antibody (STJ116369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-AKT1-S473 antibody [7W3-W4-S4] (STJA0033682)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ChIP/IP

Reactivity :

Human

€269.40 - €383.40

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Anti-Phospho-AKT1-S473 antibody [P8H9] (STJA0019074)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/AKT8 murine leukemia virus

€234.60 - €1,583.40

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Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Anti-Phospho-AKT1-S473 antibody [SAA0300] (STJA0019165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

€234.60 - €1,583.40

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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