Anti-Phospho-ACTN4-Tyr-4 antibody (N-Term) (STJA0005246)

SKU:
STJA0005246-100

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Alpha-Actinin 4-Tyr-4 (N-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN4
Gene ID: 81
Uniprot ID: ACTN4_HUMAN
Immunogen Region: N-Term
Specificity: The antibody detects a 100 kDa* protein corresponding to the molecular mass of Alpha-actinin 4 on SDS-PAGE immunoblots of human A431 cells treated with pervanadate, but is not observed after akaline phosphatase treatment.
Immunogen: Phospho-Alpha-actinin 4 (Tyr-4) synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminus of human Alpha-actinin 4. This sequence is well conserved in rat and mouse Alpha-actinin 4, but is not conserved in other Al
Tissue Specificity Widely expressed.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Protein Name Alpha-Actinin-4
Non-Muscle Alpha-Actinin 4
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-373753
Cellular Localisation Nucleus
Cytoplasm
Cell Junction
Cytoskeleton
Stress Fiber
Perinuclear Region
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Expressed In The Perinuclear Rim And Manchette Structure In Early Elongating Spermatids During Spermiogenesis
Nuclear Translocation Can Be Induced By The Pi3 Kinase Inhibitor Wortmannin Or By Cytochalasin D
Exclusively Localized In The Nucleus In A Limited Number Of Cell Lines (Breast Cancer Cell Line Mcf-7
Oral Floor Cancer Imc-2
And Bladder Cancer Ku-7)
Alternative Antibody Names Anti-Alpha-Actinin-4 antibody
Anti-Non-Muscle Alpha-Actinin 4 antibody
Anti-ACTN4 antibody

Information sourced from Uniprot.org

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