Post Translational Modifications

Primary Antibodies

Post Translational Modifications

Site-specific antibodies for acetylation and methylation research

Post-translational modifications, or PTMs, are essential eukaryotic mechanisms used to diversify protein functions and coordinate signalling networks. They are accomplished by covalent addition of protein functional groups, degradation of whole proteins, or proteolytic cleavage of regulatory subunits.

Our range lists over 4,600 individual PTM antibodies, targeting defined acetylation and methylation sites and validated across a range of application formats.

How it works

How PTMs work

A post-translational modification changes a protein after it has been synthesised, either by covalently attaching a functional group to a specific residue, by degrading the whole protein, or by proteolytically cleaving off a regulatory subunit. Antibodies in our range are raised against these specific modified sites, allowing detection of the modified form of a target independently of its unmodified counterpart.

Category benefits

Why use our PTM antibodies?

✓ Residue-level specificity
Many antibodies in our range target a defined modified residue, such as a specific acetylated lysine (for example Acetyl-BRD4-K332) or 5-methylcytosine in genomic DNA, so the modified and unmodified pools of a target can be distinguished.
✓ Choice of clonality and host
Our PTM antibodies are available as both polyclonal and monoclonal formats, raised in rabbit or mouse, so a format can be matched to the sensitivity or specificity an assay needs.
✓ Validated across multiple applications
Individual products are validated for western blot, IHC-P, IF/ICC, IP, ELISA or dot blot, so the same catalogue supports work from lysate-level screening through to imaging.
✓ Extensive catalogue depth
The category lists over 4,600 individual PTM antibodies, giving broad coverage of acetylation and methylation sites across a wide range of target proteins.
Where they're used

Typical applications

✓ Tracking modification state by western blot
WB-validated site-specific antibodies let researchers compare acetylation or methylation levels of a target across treatment conditions or cell lines.
✓ Tissue and cell imaging
Antibodies validated for IHC-P or IF/ICC support visualisation of where a modified protein pool is localised within tissue sections or fixed cells.
✓ Immunoprecipitation of modified protein complexes
IP-validated clones allow a specific PTM-bearing form of a target to be pulled down for downstream analysis of its interaction partners or further modification status.
✓ ELISA and dot-blot screening
ELISA- and dot blot-validated antibodies, including 5-methylcytosine detection in genomic DNA preparations, support quantitative or rapid qualitative screening of modification status across sample sets.

Need help choosing an antibody?

Our technical support team can help with selecting an antibody for your modified target of interest. Each product page includes a downloadable datasheet and SDS, and orders are dispatched on overnight priority shipping with gel ice packs.

Looking for more primary antibodies?

Explore our wider primary antibody range, spanning chimeric, recombinant, conjugated, tag and CD marker antibodies.

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