Anti-Recombinant-Phospho-MLKL-S358 antibody [RM2J17] (STJA0025783)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Phospho-Mixed Lineage Kinase Domain-Like Protein-S358 for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2J17 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IHC: 1:100-1:200, WB: 1:1000-1:2000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | MLKL |
| Gene ID | 197259 |
| Uniprot ID | MLKL_HUMAN |
Additional Info
| Function | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function. |
| Protein Name | Mixed Lineage Kinase Domain-Like ProteinHmlkl |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-5213460Reactome: R-HSA-5675482Reactome: R-HSA-9686347 |
| Cellular Localisation | CytoplasmCell MembraneNucleusLocalizes To The Cytoplasm And Translocates To The Plasma Membrane On Necroptosis InductionLocalizes To The Nucleus In Response To Orthomyxoviruses Infection |
| Alternative Antibody Names | Anti-Mixed Lineage Kinase Domain-Like Protein antibodyAnti-Hmlkl antibodyAnti-MLKL antibody |
Information sourced from Uniprot.org