Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of NIH/3T3, using Phospho-KAP1/TRIM28-S824 antibody (STJ11105864) at 1:5000 dilution. NIH/3T3 cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-TRIM28-S824 antibody [S5864RM] (STJ11105864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-TrkA/TrkB-Tyr516 antibody [PT1381R] (STJA0040021)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-Tuberin-Thr1462 antibody [PT1415R] (STJA0040368)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Anti-Phospho-Tyk2-Tyr1054/1055 antibody [PT1377R] (STJA0040020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Anti-Phospho-Tyrosine antibody [PT1078R] (STJA0039984)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-ULK1-S555-S555 antibody [SRM15138] (STJ11109868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-ULK1-Ser556 antibody [PT0907R] (STJA0039780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-ULK1-Ser757 antibody [PT0739R] (STJA0039237)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various lysates using Phospho-VANGL2-S79/S82/S84 Rabbit mAb (STJ11103744) at1:1000 dilution. HeLa and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-VANGL2-S79/S82/S84 antibody [S4MR] (STJ11103744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using Phospho-VANGL2-T78/S79/S82 Rabbit mAb (STJ11103743) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-VANGL2-T78/S79/S82 antibody [S3MR] (STJ11103743)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105906) at 1:4000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-VASP-S157 antibody [S5906RM] (STJ11105906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105907) at 1:3000 dilution. HEL cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-VASP-S157 antibody [S5907RM] (STJ11105907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-VASP-S239 antibody [SRM13699] (STJ11108429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-Phospho-VASP-Ser239 antibody [PT1281R] (STJA0040080)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from A-431 cells using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb (STJ11107732) at 1:3900 dilution incubated overnight at 4C. A-431 cells were treated by EGF (50 ng/ml) at 37C for 30 minutes after serum-starvation overnight. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 1s.

Anti-Phospho-VEGF Receptor 2-Y1175 antibody [SRM5543] (STJ11107732)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-YAP-Ser127 antibody [PT0743R] (STJA0039239)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-YAP-Ser397 antibody [PT1357R] (STJA0040087)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-YWHAZ-Ser58+YWHAH-Ser59+YWHAG-Ser59+YWHAB-Ser60-Ser58+Ser59+Ser59+Ser60 antibody [SRM15148] (STJ11109878)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-Zap-70/Syk-Tyr319/Tyr352 antibody [PT0710R] (STJA0039240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£285.00 - £540.00

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Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit monoclonal antibody (STJ11105888) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-ZAP70-Y493 antibody [S5888RM] (STJ11105888)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospholamban antibody [SRM14672] (STJ11109402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Mouse/Rat

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Western blot analysis of extracts of various cell lines, using PHOX2B Rabbit monoclonal antibody (STJ11102326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PHOX2B antibody (215-314) [S6MR] (STJ11102326)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Formalin-fixed, paraffin-embedded human neuroblastoma stained with anti-PHOX2B antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Anti-PHOX2B antibody [ZR292] (STJ180589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£181.50 - £703.50

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Anti-PHYHD1 antibody [PT0782R] (STJA0039343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC

Reactivity :

Human

£150.00 - £540.00

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Anti-PI3 Kinase Class 3 antibody [PT0683R] (STJA0039216)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemistry analysis of paraffin-embedded rat spleen using PI3 Kinase p110 delta Rabbit monoclonal antibody (STJ11103107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PI3 Kinase p110 delta antibody (200-300) [S7MR] (STJ11103107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-PI3 Kinase p85 alpha antibody [SRM15073] (STJ11109803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using PI3 Kinase p85 alpha/beta Rabbit monoclonal antibody (STJ11104858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PI3 Kinase p85 alpha/beta antibody (400-500) [S4858RM] (STJ11104858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-PI3 Kinase p85 alpha/beta antibody [SRM5120] (STJ11106938)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Confocal imaging of NIH/3T3 cells using PI3KP85 Alpha/P55 Gamma/P85 Beta Rabbit monoclonal antibody (STJ11104970, dilution 1:200) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.

Anti-PI3 Kinase p85 alpha/beta/gamma mAb antibody (400-500) [S4970RM] (STJ11104970)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£143.50 - £618.50

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Anti-PI3-Kinase p110 Alpha antibody [PT0873R] (STJA0039138)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-PI3-Kinase p110 Beta antibody [PT0533R] (STJA0039179)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-PI3-Kinase p110 Gamma antibody [PT1040R] (STJA0039957)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-PI3-Kinase p85 Alpha antibody [PT0082R] (STJA0039750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-PI3K-gamma antibody [SRM15013] (STJ11109743)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit monoclonal antibody (STJ11102146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PIAS1 antibody (3-101) [S6MR] (STJ11102146)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates using PIAS1+PIAS2 Rabbit mAb (STJ11102524) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PIAS1/PIAS2 antibody (552-651) [S4MR] (STJ11102524)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using PIAS2 Rabbit monoclonal antibody (STJ11102291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PIAS2 antibody (50-150) [S1MR] (STJ11102291)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Anti-PIEZO1 antibody [PT1398R] (STJA0040362)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat

£150.00 - £540.00

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Immunoprecipitation analysis of 600 Mu g extracts of Rat brain cells using 3 Mu g PIK3C3/VPS34 antibody (STJ11101401). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (STJ11101401) at a dilution of 1:500.

Anti-PIK3C3 antibody (788-887) [S1MR] (STJ11101401)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various lysates using PI3 Kinase p110 beta Rabbit monoclonal antibody (STJ11103834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3CB antibody (1-100) [S3834RM] (STJ11103834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-PIK3CB antibody (410-600) [S4849RM] (STJ11104849)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of PI3 Kinase p110 gamma in K562 lysates using PI3 Kinase p110 gamma antibody.

Anti-PIK3CG antibody (3-17 aa) [R09-5C8] (STJA0011789)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£264.50

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Western blot analysis of various lysates, using PI3 Kinase p85 alpha rabbit monoclonal antibody (STJ11102215) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R1 antibody (1-100) [S5MR] (STJ11102215)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunocytochemistry analysis of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody, and DAPI (blue)

Anti-PIK3R1 antibody (601-619 aa) [R03-3C7] (STJA0013015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£264.50

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Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 beta antibody (STJ11102171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R2 antibody (100-200) [S1MR] (STJ11102171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PI 3 Kinase p85 beta antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PIK3R2 antibody (140-164 aa) [R06-5H3] (STJA0014501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Rat

£264.50

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Western blot analysis of extracts of various cell lines, using PIK3R3/p55PIK Rabbit monoclonal antibody (STJ11101979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIK3R3 antibody (362-461) [S9MR] (STJ11101979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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